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A method for enzymatically preparing diglycerides

A technology for the preparation of diacylglycerol and enzymatic method, which is applied in the field of oil processing, can solve the problems of increasing risks, achieve the effects of reducing energy consumption, good economic benefits and industrial application prospects, and saving production and maintenance costs

Active Publication Date: 2022-03-29
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, when DAG is prepared by enzymatic hydrolysis, glycerolysis and esterification, the reaction product contains more by-products such as MAG and fatty acids, and the temperature required for molecular distillation and purification is higher, which increases the risk factor of lipids. Risks such as glycidyl esters, chloropropanol esters, and trans fatty acids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Weigh 100g of a mixture of soybean oil and absolute ethanol and place it in a 500mL round bottom flask (the molar ratio of soybean oil to absolute ethanol is 1:60), stir to mix evenly, heat to 25°C and connect to a reflux device. Add 3g of Novozym 435 therein again, and after 120min of reaction, recover the immobilized lipase, and remove absolute ethanol on a rotary evaporator. Using the above reaction product as the substrate, add absolute ethanol (the molar ratio of monoglyceride to absolute ethanol is 1:1.5), stir to mix evenly, heat to 30°C and connect the reflux device; then add 400U / g immobilized MGLP II, after 0.5h of reaction, recover the immobilized lipase, and remove absolute ethanol on a rotary evaporator. Finally, molecular distillation (evaporating surface temperature is 120°C) was used to separate and purify DAG. The content of DAG in the product was determined to be 58.63%, and lipids such as fatty acid ethyl ester, glycidyl ester, chloropropanol ester a...

Embodiment 2

[0032] Weigh 100g of the mixture of soybean oil and absolute ethanol and place it in a 500mL round-bottomed flask (the molar ratio of soybean oil to absolute ethanol is 1:40), stir to mix evenly, heat to 30°C and connect the reflux device. Then add 6g of Lipozyme 435 therein, and after 60 minutes of reaction, recover the immobilized lipase, and remove absolute ethanol on a rotary evaporator. Using the above reaction product as the substrate, add absolute ethanol (the molar ratio of monoglyceride to absolute ethanol is 1:1), stir to mix evenly, heat to 40°C and connect the reflux device; then add 100U / g immobilized MGLP II, after 2 hours of reaction, recover the immobilized lipase, and remove absolute ethanol on a rotary evaporator. Finally, molecular distillation (evaporating surface temperature is 110°C) was used to separate and purify DAG. The content of DAG in the product was determined to be 51.24%, and lipids such as fatty acid ethyl ester, glycidyl ester, chloropropanol...

Embodiment 3

[0034] Weigh 100g of the mixture of seaweed oil and absolute ethanol and place it in a 500mL round bottom flask (the molar ratio of soybean oil to absolute ethanol is 1:100), stir to mix evenly, heat to 40°C and connect the reflux device. Then add 10g of Novozym 435 therein, and after 15 minutes of reaction, recover the immobilized lipase, and remove absolute ethanol on a rotary evaporator. Using the above reaction product as the substrate, add absolute ethanol (the molar ratio of monoglyceride to absolute ethanol is 1:3), stir to mix evenly, heat to 50°C and connect the reflux device; then add 200U / g immobilized MGLP II, after 1 hour of reaction, recover the immobilized lipase, and remove absolute ethanol on a rotary evaporator. Finally, molecular distillation (evaporating surface temperature is 120°C) was used to separate and purify DAG. The content of DAG in the product was determined to be 54.39%, and lipids such as fatty acid ethyl ester, glycidyl ester, chloropropanol e...

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PUM

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Abstract

The invention discloses a method for preparing diglyceride by enzymatic method, which belongs to the technical field of oil processing. The first step uses immobilized lipase to catalyze the alcoholysis of oil, and then uses the immobilized monoglyceride lipase to catalyze the alcoholysis of the by-product monoglyceride of the first step reaction. After the two-step alcoholysis reaction, the monoglyceride is completely removed, and the reaction by-product is only fatty acid ethyl ester. Compared with the by-products being monoglycerides and fatty acids, when the by-products are fatty acid ethyl esters for molecular distillation and purification, the required purification temperature is lower, which not only reduces energy consumption, but also avoids the generation of lipid risk factors during the purification process. Improve product quality; at the same time reduce equipment requirements, save production and maintenance costs, and have good economic benefits and industrial application prospects.

Description

technical field [0001] The invention belongs to the technical field of oil processing, and in particular relates to a method for preparing diglyceride by enzymatic method. Background technique [0002] Diacylglycerol (DAG) is a natural component in oil, and its content is low (<10%) in most animal and vegetable oils. DAG is a substance generally recognized as safe (GRAS, Generally Recognized as Safe), safe to eat, and its taste, color, and flavor are similar to triacylglycerol (TAG). However, compared with TAG, it has a different metabolic pathway and will not Re-esterify in the body to generate TAG, but provide energy for the body's metabolism through β-oxidation, so DAG can effectively prevent the accumulation of fat in the body, and has the effect of losing weight. At present, many studies on the physiological function of DAG have shown that DAG also has the functions of reducing postprandial blood lipid, preventing cardiovascular and cerebrovascular diseases, and imp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/6458
CPCC12P7/6454
Inventor 李道明钟小荣周端刘宁
Owner SHAANXI UNIV OF SCI & TECH
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