Genes regulating plant flowering time and biomass and their utilization
A technology of flowering time and biomass, applied in the field of plant biology, can solve problems such as limited utilization prospects
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Embodiment 1
[0133] Example 1. A semi-dominant late-flowering mutant edf1 was obtained through EMS mutagenesis screening
[0134] The common inbred line W22 of maize was selected as the mutagenesis material, the mutant library was established by pollen EMS mutagenesis, and the late flowering mutant edf1 was obtained by phenotypic screening. Crossing EW0019 from the W22 background with W64A, in the F2 segregation population, the inventors found that the flowering time of its mutant was about two weeks later than that of the wild type; the flowering time of the heterozygous plants was between the wild type and the pure Synzygous mutants showed a certain degree of late flowering phenotype. This shows that edf1 is a semi-dominant late-flowering mutant ( figure 1 A, C).
[0135] edf1 under long daylight conditions in spring (March 15 to July 15) and autumn (July 25 to October 25) in Shanghai and winter (November 1 to March 1) in Sanya, Hainan Under short-day conditions, all showed late flowe...
Embodiment 2
[0137] Example 2, edf1 causes late flowering phenotype by affecting the expression of maize florigen ZCN8
[0138] By tracking the development process of the mutant, the inventors found that, consistent with the late flowering phenotype of the mutant, the transition period of the apical meristem and the peak expression of florigen ZCN8 were two weeks later than that of the wild type about( figure 2 A). This test result suggested that the late flowering phenotype of the edf1 mutant was due to the affected normal expression of ZCN8.
[0139] The present inventors also detected the expression pattern of ZCN8 within a day when the fifth leaf of edf1 and WT after germination was fully unfolded under long-day sunshine conditions in Shanghai, and the results were as follows: figure 2 B, Wild type fluctuates but relatively high expression occurs throughout the day, whereas edf1 is expressed at very low levels. It shows that when the fifth leaf is fully unfolded after germination,...
Embodiment 3
[0141] Cloning of embodiment 3, edf1 mutant gene
[0142] Through map-based cloning combined with whole-genome sequencing, the inventors located the candidate gene numbered GRMZM2G052544 ( image 3 A).
[0143] Genomic DNA sequence analysis of GRMZM2G052544 found that the 8th base of the second intron was mutated from guanine G to adenine A, which together with the latter base formed a new 5' splicing site of AGGT , this change causes it to interfere with the selection of the original splice site. The first 9 bases in the second intron are retained in the newly formed transcript ( image 3 B, C). Using RT-PCR detection, it was found that this form of transcripts accounted for the majority, while the original transcripts were still expressed, but the detected amount was significantly less than that of the mutated form ( image 3 D, E).
[0144] The inventor searched MaizeGDB (https: / / www.maizegdb.org), a corn bioinformatics website, and found the mutation site in the inbre...
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