A kind of composition and its preparation method and application
A composition and mixture technology, applied in the direction of botany equipment and methods, applications, chemicals for biological control, etc., can solve the problems of mammalian poisoning, chromosomal aberration, human and animal harm, etc., and achieve easy industrial expansion production, easy Acquisition, low-cost effect
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Embodiment 1
[0041] Example 1 Preparation of antibacterial substances for preventing and treating silkworm morbidity
[0042] Step (1): After the wheat bran is dried at 70-80°C, it is crushed into wheat bran powder, and passed through a 30-mesh sieve for later use;
[0043] Step (2): Extract the wheat bran powder in step (1) with 95% ethanol for 24 hours each time, with occasional shaking during the period, and repeat the extraction twice;
[0044] Step (3): Separate the ethanol extract from the wheat bran by filtration, combine the filtrates, and concentrate under reduced pressure to obtain a viscous ethanol extract, wherein the concentration under reduced pressure is at -0.08MPa and placed in a 60°C water bath Concentration process;
[0045] Step (4): Extract the ethanol extract with petroleum ether, each extraction is 15 minutes, repeat twice, and combine the petroleum ether extracted each time;
[0046] Step (5): Concentrating the petroleum ether phase under reduced pressure to obtai...
Embodiment 2
[0052] Embodiment 2 Preparation of antibacterial substances for preventing and treating silkworm morbidity
[0053] Step (1): Dry the wheat bran at 70-80°C, beat it into wheat bran powder, pass through a 40-mesh sieve for later use;
[0054] Step (2): Extract the wheat bran powder in step (1) with 95% ethanol for 24 hours each time, and repeat the extraction 3 times with occasional shaking during the period;
[0055] Step (3): Separate the ethanol extract from the wheat bran by filtration, combine the filtrates, and concentrate under reduced pressure to obtain a viscous ethanol extract, wherein the concentration under reduced pressure is at -0.08MPa and placed in a 55°C water bath Concentration process;
[0056] Step (4): extract the ethanol extract with petroleum ether, each extraction lasts 20 minutes, repeat 3 times, and combine the petroleum ether extracted each time;
[0057] Step (5): Concentrating the petroleum ether phase under reduced pressure to obtain a pasty whea...
Embodiment 3
[0063] Example 3 Preparation of antibacterial substances for preventing and treating silkworm morbidity
[0064] Step (1): Dry the wheat bran at 70-80°C, beat it into wheat bran powder, pass through a 40-mesh sieve for later use;
[0065] Step (2): Extract the wheat bran powder in step (1) with 95% ethanol for 24 hours each time, and repeat the extraction 3 times with occasional shaking during the period;
[0066] Step (3): Separate the ethanol extract from the wheat bran by filtration, combine the filtrates, and concentrate under reduced pressure to obtain a viscous ethanol extract, wherein the concentration under reduced pressure is at -0.08MPa and placed in a 50°C water bath Concentration process;
[0067] Step (4): extracting the ethanol extract with petroleum ether, each extraction was 30 minutes, repeated 3 times, and combining the petroleum ether extracted each time;
[0068] Step (5): Concentrating the petroleum ether phase under reduced pressure to obtain a pasty wh...
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