SNP marker closely linked with continuous multi-leaf-position leaf width major QTL under corn tassels and application of SNP marker

Abstract

The invention relates to the technical field of genetic engineering and molecular biology, in particular to an SNP marker closely linked with continuous multi-leaf-position leaf width main effect QTLunder corn tassels and application of the SNP marker. The SNP marker closely linked with the continuous multi-leaf-position leaf width main effect QTL under the corn tassels is chr2-208336, and the site mutation is A / G; and the physical position refers to a reference genome B73 RefGen-v4 version of a maize selfing line B73. Auxiliary selection is carried out through the SNP marker, the width of the continuous four-leaf width character under the corn tassels in the mature period can be predicted only by detecting SNP bases providing specific sites of primer PCR amplification products, the identification method is simple, and the selection efficiency is high. Single corn plants with continuous four leaves of wide leaves and narrow leaves under tassels in the mature period can be identified before corn sowing or in the early growth period, can be used for narrow-leaf dense-tolerant corn breeding and wide-leaf silage corn breeding respectively, are clear in selection target, are not influenced by the environment A feasible way is provided for molecular assisted breeding of narrow-leaf dense-resistant and wide-leaf silage corn.

Description

technical field [0001] The invention relates to the technical fields of genetic engineering and molecular biology, and the specific field is a SNP marker closely linked with the main effect QTL of the continuous multi-leaf position leaf width under the tassel of maize and its application. Background technique [0002] Corn is a major food and feed and industrial raw material crop. In 2014, it became the crop with the largest planting area in China, and it occupies a very important position in China's agricultural production and national economic development. [0003] The practice of breeding proves that the increase in the yield per unit area of ​​corn is due to the increase in the adaptability of corn to tolerance to dense planting (Duvick, D., 2005 Genetic progress in yield of United States maize (Zea mays L.). Maydica 50:193.). But under high-density planting conditions, blades will be mutually shaded, especially the wider blades at the top, which seriously affects venti...

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Problems solved by technology

Some studies on the leaf width traits of different leaf positions in maize have also shown that the leaf width of different leaf positions is regulated by one or several common QTLs, and there are also different QTLs. The genetic basis is very complicated.

Tian(Tian,F.,P.J.Bradbury,P.J.Brown,H.Hung,Q.Sun et al.,2011Genome-wide association study of leafarchitecture in the maize nested association mapping population.Nat Genet 43:159-162.) and Yang( Yang, N., Y.Lu, X.Yang, J.Huang, Y.Zhou et al., 2014 Genome wide association studies using a new nonparametric model reveal the genetic architecture of 17agronomic traits in an enlarged maize association panel.PLoSGenet10:e1004573.) etc. A number of SNP loci that were significantly associated with leaf width were identified by association analysis, and they were distributed on multiple chromosomes in maize. Study on Positioning of Leaf Width in Multi-leaf Position

In addition, limited by the type of mapping population, the type of markers used, the accuracy of the genetic linkage map, and environmental control, the QTL analysis of maize leaf width, especially the continuous multi-leaf position under the tassel, has no consistent results. Application of QTL Markers for Leaf Width at Multi-leaf Position Under Panicle

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