SNP marker closely linked with continuous multi-leaf-position leaf width major QTL under corn tassels and application of SNP marker

A maize, compact technology, applied in the fields of genetic engineering and molecular biology, can solve the problem of the application of QTL markers that have not yet controlled the multi-leaf position under the tassel, the genetic basis is complex, and the continuous multi-leaf position under the tassel has not been carried out. Widely positioned research and other issues, to achieve the effect of simple identification method, high selection efficiency, and clear selection goals

Active Publication Date: 2020-12-04
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Some studies on the leaf width traits of different leaf positions in maize have also shown that the leaf width of different leaf positions is regulated by one or several common QTLs, and there are also different QTLs. The genetic basis is very complicated.
Tian(Tian,F.,P.J.Bradbury,P.J.Brown,H.Hung,Q.Sun et al.,2011Genome-wide association study of leafarchitecture in the maize nested association mapping population.Nat Genet 43:159-162.) and Yang( Yang, N., Y.Lu, X.Yang, J.Huang, Y.Zhou et al., 2014 Genome wide association studies using a new nonparametric model reveal the genetic architecture of 17agronomic traits in an enlarged ma

Method used

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  • SNP marker closely linked with continuous multi-leaf-position leaf width major QTL under corn tassels and application of SNP marker
  • SNP marker closely linked with continuous multi-leaf-position leaf width major QTL under corn tassels and application of SNP marker
  • SNP marker closely linked with continuous multi-leaf-position leaf width major QTL under corn tassels and application of SNP marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Mapping analysis of the main effect QTL of leaf width in continuous multi-leaf position under maize tassel

[0029] 1. RIL genetic segregation and mapping population construction

[0030] Through germplasm resource screening research, it is clear that L027 has narrow leaves and excellent comprehensive traits, and can be used as a parent for excavation and utilization of narrow leaves and other excellent traits. After the combination of L027 and Su 95-1 was crossed, 7 consecutive generations of selfing plus generation were carried out in Nanjing, Jiangsu and Sanya, Hainan by single-grain transmission method, and the RIL genetic segregation and positioning population was successfully constructed, which included 379 families in total.

[0031] 2. Population phenotype analysis

[0032] Families including population parents (L027 and Su 95-1) were sown in Sanya, Hainan in winter in 2016, 2017, 2018 and 2019 (2 replicates). At the widest position of the second, th...

Embodiment 2

[0041] Example 2 Application of SNP Markers Closely Linked to Major QTL Markers for Continuous Multi-Leaf Position and Leaf Width Under the Tassel of Maize

[0042] The SNP marker closely linked to the main effect QTL of continuous multi-leaf width under the maize tassel is chr2_208336, and the genomic DNA of the material to be identified is used as a template to provide primers for PCR amplification; the nucleotide sequence of the primers is as shown in SEQ ID NO: 1 -2 shown. The sequence of the amplified product is shown in SEQ ID NO: 3-4.

[0043] The specific steps for using the above-mentioned SNP markers to assist in judging the continuous multi-leaf width under the tassel of maize are as follows:

[0044] (1) Using the CTAB method to extract the genomic DNA of the material to be identified

[0045] Under liquid nitrogen or vacuum-dried corn leaves, quickly grind into powder and transfer about 0.5-1g to a 2ml centrifuge tube, add 800μL 65°C preheated CTAB buffer and mi...

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Abstract

The invention relates to the technical field of genetic engineering and molecular biology, in particular to an SNP marker closely linked with continuous multi-leaf-position leaf width main effect QTLunder corn tassels and application of the SNP marker. The SNP marker closely linked with the continuous multi-leaf-position leaf width main effect QTL under the corn tassels is chr2-208336, and the site mutation is A/G; and the physical position refers to a reference genome B73 RefGen-v4 version of a maize selfing line B73. Auxiliary selection is carried out through the SNP marker, the width of the continuous four-leaf width character under the corn tassels in the mature period can be predicted only by detecting SNP bases providing specific sites of primer PCR amplification products, the identification method is simple, and the selection efficiency is high. Single corn plants with continuous four leaves of wide leaves and narrow leaves under tassels in the mature period can be identified before corn sowing or in the early growth period, can be used for narrow-leaf dense-tolerant corn breeding and wide-leaf silage corn breeding respectively, are clear in selection target, are not influenced by the environment A feasible way is provided for molecular assisted breeding of narrow-leaf dense-resistant and wide-leaf silage corn.

Description

technical field [0001] The invention relates to the technical fields of genetic engineering and molecular biology, and the specific field is a SNP marker closely linked with the main effect QTL of the continuous multi-leaf position leaf width under the tassel of maize and its application. Background technique [0002] Corn is a major food and feed and industrial raw material crop. In 2014, it became the crop with the largest planting area in China, and it occupies a very important position in China's agricultural production and national economic development. [0003] The practice of breeding proves that the increase in the yield per unit area of ​​corn is due to the increase in the adaptability of corn to tolerance to dense planting (Duvick, D., 2005 Genetic progress in yield of United States maize (Zea mays L.). Maydica 50:193.). But under high-density planting conditions, blades will be mutually shaded, especially the wider blades at the top, which seriously affects venti...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 刘瑞响崔亚坤张美景陈艳萍袁建华王森郑飞赵文明孔令杰孟庆长
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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