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Method and system for treating type-2 diabetes mellitus by black tartary buckwheat stem and leaf extracts

A technology of type 2 diabetes and black tartary buckwheat stems and leaves, which is applied in the direction of medical formula, plant raw materials, medical preparations containing active ingredients, etc., can solve problems such as high cost, and achieve good promotion and feasibility effects

Inactive Publication Date: 2020-12-08
WENZHOU MEDICAL UNIV
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  • Application Information

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Problems solved by technology

[0008] The present invention overcomes the high-cost problem of β-cell gene knockout mice by constructing a type 2 diabetes mouse model, and simplifies the experiment for verifying the therapeutic effect of the stem and leaf extract of black bitter mustard on obesity-related diseases (such as type 2 diabetes)

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  • Method and system for treating type-2 diabetes mellitus by black tartary buckwheat stem and leaf extracts
  • Method and system for treating type-2 diabetes mellitus by black tartary buckwheat stem and leaf extracts

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Embodiment Construction

[0009] 1. Preparation of type 2 diabetes mouse model, group processing and histological examination

[0010] 40 12-week-old male C57BL / 6 mice (purchased from Shanghai Experimental Animal Center, Chinese Academy of Sciences), 40 SPF male C57BL / 6 mice (adaptive feeding for 1 week, randomly divided into 10 normal control NC groups) And the experimental group 30, the NC group was given conventional feed, the experimental group was given high sugar and high fat (MD12031 (gm% / kacl%) protein 19.2 / 20 fat 4.30 / 10 carbohydrate 67.3 / 70 trace elements 9.20 / 0) diet feeding. After 5 weeks, fast for 12 hours, intraperitoneally inject 1% streptozotocin 40mg / kg to induce diabetes model, fast for 4 hours after 72 hours, and take blood to measure FPG. Blood glucose concentration> 11.8mmol / L, and polydipsia, The symptoms of polyphagia and polyuria suggested that the diabetic mouse model was successfully induced. The stems and leaves of black bitter mustard were divided into model control (DM) gro...

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Abstract

The research is different from other previous research, and an action mechanism of black tartary buckwheat stem and leaf extracts on treatment of type-2 diabetes mellitus is discussed by using a biological method. Skeletal muscle cells are used as experimental subjects, an experimental group for blocking a P13 / Akt signal path is constructed, and then the black tartary buckwheat stem and leaf extracts are given to analyze liver cell related saccharide gene expression, and discuss a cellular mechanism of the black tartary buckwheat stem and leaf extracts on treatment of type-2 diabetes mellitus.At present, the research is not reported at home and abroad. Therefore, a novel treatment method is provided through the black tartary buckwheat stem and leaf extracts. By using the method, a lot ofexpenditures for purchasing db / db mice and double-gene knockout mice can be saved. According to the research, the protein expression level of an insulin receptor substrate P-IRS-1 is measured, and then the influence of the black tartary buckwheat stem and leaf extracts on the insulin sensitivity of a mouse myoblast cell strain (C2C12) is detected, so that a theoretical basis is provided for treating type-2 diabetes mellitus.

Description

technical field [0001] The field of treating obesity-related diseases (such as type 2 diabetes) by the stem and leaf extract of black tartary buckwheat, especially involves verifying the feasibility of treating type 2 diabetes by the stem and leaf extract of black tartary buckwheat. Background technique [0002] Studies have shown that diabetes has become an endocrine and metabolic disease that seriously threatens human health. Type II diabetes accounts for more than 95% of diabetic patients. The risk of malignant tumors such as colorectal cancer, liver cancer, and breast cancer in patients with type II diabetes is significantly higher than that of the general population. Insulin resistance is a common cause of diseases such as type II diabetes and cardiovascular disease in obese and susceptible individuals. [0003] The current treatment of diabetes mainly relies on drugs. Although it can control blood sugar at a relatively stable low level, long-term use will inevitably ...

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Application Information

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IPC IPC(8): A01K67/02A61K36/70A61K127/00A61K135/00
CPCA01K67/02A01K2207/25A01K2227/105A01K2267/0362A61K36/70
Inventor 肖敏
Owner WENZHOU MEDICAL UNIV