Special fat powder for marine fishes and preparation method and application of special fat powder
A seawater fish and fat powder technology, which is applied in the direction of edible oil/fat, application, food science, etc., can solve the problems of not significantly reducing the quality of seawater fish fatty liver culture, so as to improve the quality of culture, promote growth and development, and improve the preparation process simple effect
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preparation example 1
[0052] This preparation example provides a kind of microbial grease I, and its preparation method is as follows:
[0053] (1) Thraustochytrium was inoculated in the fermentation medium with an inoculation amount of 10%, and fermented at 35° C. with an initial pH value of 6.0 for 72 hours to obtain a fermented liquid, which was filtered to obtain a concentrated fermented liquid; wherein the fermented medium The formula consists of glucose 50-60g / L, corn syrup 1-5g / L, potassium dihydrogen phosphate 0.1-0.5g / L, sodium chloride 10-15g / L, and the balance is water;
[0054] (2) Swell the concentrated fermented liquid obtained in step (1) at 50°C for 18 hours, and then dry at 80°C to obtain dry bacteria powder;
[0055] (3) The dry bacterial powder obtained in step (2) is crushed and broken, extracted with n-hexane for 8 hours, and 8 L is used for every kg of broken dry bacterial powder, and then vacuum precipitation to obtain the microbial oil.
preparation example 2
[0057] This preparation example provides a kind of microbial grease II, and its preparation method is as follows:
[0058] (1) Thraustochytrium was inoculated in the fermentation medium with an inoculation amount of 10%, and fermented for 100 hours at 30°C with an initial pH value of 5.0 to obtain a fermented liquid, which was filtered to obtain a concentrated fermented liquid; wherein the fermented medium The formula consists of glucose 50-60g / L, corn syrup 1-5g / L, potassium dihydrogen phosphate 0.1-0.5g / L, sodium chloride 10-15g / L, and the balance is water;
[0059] (2) Swell the concentrated fermented liquid obtained in step (1) at 40°C for 24 hours, and then dry at 75°C to obtain dry bacteria powder;
[0060] (3) The dried bacteria powder obtained in step (2) was crushed to break the wall, extracted with methanol for 6 h, and 10 L was used per kg of the broken wall dried bacteria powder, and then vacuum precipitation was carried out to obtain the microbial oil.
preparation example 3
[0062] This preparation example provides a kind of microbial grease III, and its preparation method is as follows:
[0063] (1) Thraustochytrium was inoculated in the fermentation medium with an inoculation amount of 10%, and fermented at 40° C. with an initial pH value of 7.4 for 80 hours to obtain a fermented liquid, which was filtered to obtain a concentrated fermented liquid; wherein the fermented medium The formula consists of glucose 50-60g / L, corn syrup 1-5g / L, potassium dihydrogen phosphate 0.1-0.5g / L, sodium chloride 10-15g / L, and the balance is water;
[0064] (2) Swell the concentrated fermented liquid obtained in step (1) at 60°C for 12 hours, and then dry at 85°C to obtain dry bacteria powder;
[0065] (3) The dried bacteria powder obtained in step (2) was crushed to break the wall, extracted with petroleum ether for 12 hours, and 5 L was used for every kg of the broken wall dried bacteria powder, and then vacuum precipitated to obtain the microbial oil.
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