Eucalyptus citriodora essential oil/nisin nano-emulsion and preparation method thereof

A nisin and nanoemulsion technology is applied in the field of lemon eucalyptus essential oil/nisin nanoemulsion and preparation, which can solve the problems of low preparation efficiency and insufficient stability of the nanoemulsion, and achieves widening the bacteriostatic range and good Application prospects and market value, the effect of overcoming application shortcomings

Pending Publication Date: 2020-12-15
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, there are very few patent applications about the nanoemulsion of bacteriostatic type encapsulating plant essential oils. At present, only the Chinese patent CN108719457A application discloses a nanoemulsion with bacteriostatic activity thymol and its preparation method and application. Although nano-emulsions can be prepared, the preparation efficiency is low, and the stability of the prepared nano-emulsions is not strong enough

Method used

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  • Eucalyptus citriodora essential oil/nisin nano-emulsion and preparation method thereof
  • Eucalyptus citriodora essential oil/nisin nano-emulsion and preparation method thereof
  • Eucalyptus citriodora essential oil/nisin nano-emulsion and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Particle diameter, PDI (polydispersity index) and Zeta potential measurement of embodiment 1 lemon eucalyptus essential oil / nisin nanoemulsion

[0026] 1.1 Experimental materials:

[0027] Tween 80: AR; Sinopharm Chemical Reagent Co., Ltd.

[0028] Lemon eucalyptus essential oil: France Florihana Co., Ltd.

[0029] Soy lecithin: AR; Sinopharm Chemical Reagent Co., Ltd.

[0030] Nisin: AR; Aladdin Chemical Reagent Co., Ltd.

[0031] Corn oil: Yihai Kerry Arowana Grain, Oil and Foodstuffs Co., Ltd.

[0032] 1.2 Experimental method:

[0033] (1) Preparation of lemon eucalyptus essential oil / nisin nanoemulsion

[0034] ① Weigh 45 mg of nisin and add it to 9 mL of distilled water, stir magnetically at room temperature for 30 min to prepare a 5 mg / mL nisin solution.

[0035] ② Add 180 mg Tween 80 and 90 mg soybean lecithin to the above solution respectively, and stir magnetically at room temperature for 30 minutes to form a water phase.

[0036] ③Pipe 600μL corn oil an...

Embodiment 2

[0042] The viscosity determination of embodiment 2 lemon eucalyptus essential oil / nisin nanoemulsion

[0043] 2.1 Experimental materials:

[0044] Lemon Eucalyptus Essential Oil / Nisin Nanoemulsion

[0045] 2.2 Experimental method:

[0046] Get the lemon eucalyptus essential oil / nisin nanoemulsion 1.3mL prepared in Example 1 and place it on the fixture of the rotational rheometer, select a tapered plate with a diameter of 40mm, and measure the shear rate at room temperature at 10-100l / s Viscosity change of the nanoemulsion in the range.

[0047] 2.3 Experimental results:

[0048] Figure 4 The results reflect that the viscosity of the prepared nanoemulsion is generally low, and the maximum viscosity is less than 0.0025 Pa·s, and with the increase of the shear rate, the viscosity of the nanoemulsion gradually decreases, indicating that the fluidity of the nanoemulsion is better.

Embodiment 3

[0049]Example 3 Determination of synergistic antibacterial performance of lemon eucalyptus essential oil / nisin nanoemulsion

[0050] 3.1 Experimental materials:

[0051] Lemon Eucalyptus Essential Oil / Nisin Nanoemulsion

[0052] Sodium chloride: AR; Sinopharm Chemical Reagent Co., Ltd.

[0053] Beef Extract: BR; Sinopharm Chemical Reagent Co., Ltd.

[0054] Fish meal peptone: BR; Sinopharm Chemical Reagent Co., Ltd.

[0055] Agar powder: BR; Sinopharm Chemical Reagent Co., Ltd.

[0056] Anhydrous disodium hydrogen phosphate: AR; Sinopharm Chemical Reagent Co., Ltd.

[0057] Potassium dihydrogen phosphate: AR; Sinopharm Chemical Reagent Co., Ltd.

[0058] Strains: E.coli O157:H7; Staphylococcus aureus

[0059] 3.2 Experimental method:

[0060] Take an appropriate amount of lemon eucalyptus essential oil / nisin nanoemulsion prepared in Example 1 and place it in a clean bench for ultraviolet sterilization treatment for 1 hour.

[0061] Inoculate E.coli O157:H7 and Staphylo...

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Abstract

The invention belongs to the field of food antibacterial and fresh-keeping materials, and particularly relates to a eucalyptus citriodora essential oil/nisin nano-emulsion and a preparation method thereof. The preparation method comprises the following steps: dissolving a certain mass of nisin in deionized water, adding a composite emulsifier composed of Tween 80 and soybean lecithin to form an aqueous phase, magnetically stirring, dropwise adding eucalyptus citriodora essential oil-loaded corn oil as an oil phase, and performing stirring homogenization and ultrasonication to form an oil-in-water type nano-emulsion. The nano-emulsion prepared by the invention can effectively improve the water solubility and biocompatibility of the eucalyptus citriodora essential oil, and can achieve the purposes of efficient utilization and long-acting antibacterial effect by virtue of the synergistic effect with the nisin, thereby expanding the application of the eucalyptus citriodora essential oil and the nisin in the field of food antibacterial and fresh-keeping materials.

Description

technical field [0001] The invention belongs to the field of food antibacterial and fresh-keeping materials, and in particular relates to a lemon eucalyptus essential oil / nisin nanoemulsion and a preparation method thereof. Background technique [0002] Lemon eucalyptus essential oil is a kind of plant essential oil extracted from the leaves of lemon eucalyptus tree. It emits a pungent aromatic smell, has strong antibacterial ability, and has broad-spectrum antibacterial properties. It is mainly used in flavors and fragrances, medicine, pest control and other fields , is a natural non-toxic additive. But lemon eucalyptus essential oil is volatile, sensitive to light, heat, oxygen, etc., and has a pungent flavor when used, so the utilization rate is low. There are very few domestic patent applications for improving the stability and utilization of lemon eucalyptus essential oil. At present, only the Chinese patent CN105030677A discloses a highly stable lemon eucalyptus esse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3544A23L3/3472A23L29/00A23L29/10A23L5/30
CPCA23L3/3544A23L3/3472A23L29/03A23L29/10A23L29/04A23L5/32A23V2002/00A23V2200/10A23V2250/18A23V2250/1842A23V2300/48
Inventor 林琳陈文庆崔海英
Owner JIANGSU UNIV
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