Plant-derived polypeptide with anti-aging effect, face cream containing plant-derived polypeptide and preparation method of face cream
A plant-derived, anti-skin aging technology, applied in the field of plant-derived polypeptides, can solve problems such as the preparation and application of sage seed polypeptides that have not been found
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Embodiment 1
[0025] Embodiment 1: Preparation of sage seed polypeptide
[0026] Grind the dried sage seeds, pass through a 60-mesh sieve, add dichloromethane under reflux for degreasing according to the ratio of solid to liquid 1:10 (g / mL), and extract twice for 2 hours each time; after evaporating the solvent, Mix the powder and distilled water according to the ratio of material to liquid 1:25 (g / mL), adjust the pH value to 8.0, stir and extract in a water bath at 50°C for 1 hour; collect the extract by filtration, centrifuge at 9000r / min for 15 minutes, and collect the supernatant And adjust the pH value to the isoelectric point of 4.7, centrifuge again at 9000r / min for 10min, collect the precipitate, and obtain the total protein of sage seeds.
[0027] Weigh an appropriate amount of sage seed total protein and dissolve it in PBS buffer at a material-to-liquid ratio of 1:25 (g / mL), adjust the pH to 8.5, and add alkaline protease in a water bath at 55°C (the amount is based on mouse The ...
Embodiment 2
[0029] Example 2: Anti-aging effect of sage seed polypeptide
[0030] 1. Test material
[0031] Human skin fibroblasts (HFBs) were purchased from Shenzhen Haodi Huatuo Biotechnology Co., Ltd. within three generations.
[0032] The test polypeptide is the sage seed polypeptide, prepared according to the method in Example 1.
[0033] DMEM medium was purchased from Thermo Fisher Scientific, and fetal bovine serum was purchased from Gibco.
[0034] RT-PCR primers were designed and synthesized by Shanghai Sangon.
[0035] 2. Test method
[0036] 2.1 Cell culture
[0037] After recovering HFBs cells, culture them in DMEM containing 10% fetal bovine serum and 1% double antibody at 37°C, 5% CO 2 Cultivated under the same conditions, and subcultured for 2-3 days.
[0038] 2.2 Preparation of aging skin cell model
[0039] In this field, HFBs within 10 passages have strong proliferative activity and are regarded as young cells, cells of 10 to 20 passages are regarded as middle-age...
Embodiment 3
[0064] Example 3: Anti-aging skin cream containing sage seed polypeptide
[0065] The formula is as follows, and the face cream containing the clary sage seed polypeptide (prepared by the method in Example 1) is prepared according to the conventional process.
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