Plant-derived polypeptide with anti-aging effect, face cream containing plant-derived polypeptide and preparation method of face cream

A plant-derived, anti-skin aging technology, applied in the field of plant-derived polypeptides, can solve problems such as the preparation and application of sage seed polypeptides that have not been found

Pending Publication Date: 2020-12-15
张超科
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there have been many reports on the use of sage extract as a cosmetic additi...

Method used

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  • Plant-derived polypeptide with anti-aging effect, face cream containing plant-derived polypeptide and preparation method of face cream
  • Plant-derived polypeptide with anti-aging effect, face cream containing plant-derived polypeptide and preparation method of face cream
  • Plant-derived polypeptide with anti-aging effect, face cream containing plant-derived polypeptide and preparation method of face cream

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Preparation of sage seed polypeptide

[0026] Grind the dried sage seeds, pass through a 60-mesh sieve, add dichloromethane under reflux for degreasing according to the ratio of solid to liquid 1:10 (g / mL), and extract twice for 2 hours each time; after evaporating the solvent, Mix the powder and distilled water according to the ratio of material to liquid 1:25 (g / mL), adjust the pH value to 8.0, stir and extract in a water bath at 50°C for 1 hour; collect the extract by filtration, centrifuge at 9000r / min for 15 minutes, and collect the supernatant And adjust the pH value to the isoelectric point of 4.7, centrifuge again at 9000r / min for 10min, collect the precipitate, and obtain the total protein of sage seeds.

[0027] Weigh an appropriate amount of sage seed total protein and dissolve it in PBS buffer at a material-to-liquid ratio of 1:25 (g / mL), adjust the pH to 8.5, and add alkaline protease in a water bath at 55°C (the amount is based on mouse The ...

Embodiment 2

[0029] Example 2: Anti-aging effect of sage seed polypeptide

[0030] 1. Test material

[0031] Human skin fibroblasts (HFBs) were purchased from Shenzhen Haodi Huatuo Biotechnology Co., Ltd. within three generations.

[0032] The test polypeptide is the sage seed polypeptide, prepared according to the method in Example 1.

[0033] DMEM medium was purchased from Thermo Fisher Scientific, and fetal bovine serum was purchased from Gibco.

[0034] RT-PCR primers were designed and synthesized by Shanghai Sangon.

[0035] 2. Test method

[0036] 2.1 Cell culture

[0037] After recovering HFBs cells, culture them in DMEM containing 10% fetal bovine serum and 1% double antibody at 37°C, 5% CO 2 Cultivated under the same conditions, and subcultured for 2-3 days.

[0038] 2.2 Preparation of aging skin cell model

[0039] In this field, HFBs within 10 passages have strong proliferative activity and are regarded as young cells, cells of 10 to 20 passages are regarded as middle-age...

Embodiment 3

[0064] Example 3: Anti-aging skin cream containing sage seed polypeptide

[0065] The formula is as follows, and the face cream containing the clary sage seed polypeptide (prepared by the method in Example 1) is prepared according to the conventional process.

[0066]

[0067]

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PUM

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Abstract

The invention discloses a plant-derived polypeptide with an anti-aging effect, face cream containing the plant-derived polypeptide and a preparation method of the face cream. The plant-derived polypeptide is a sage seed polypeptide. Skin aging is a part of body aging, is shown as skin relaxation and thinning, and is one of the most intuitive external expressions of body aging due to wrinkle formation and gradual increase and deepening. Researches show that skin aging is related to the proliferation ability of skin fibroblasts and the decrease of the content of dermal collagen. Therefore, the promotion of the proliferation of human skin fibroblasts and the synthesis of collagen is an important aspect for keeping skin young and resisting aging. The test results show that the sage seed polypeptide can promote the proliferation of human skin fibroblasts and the synthesis of collagen by human skin fibroblasts, has an anti-skin aging effect, and can be used for preparing anti-skin aging cosmetics.

Description

technical field [0001] The invention belongs to the field of cosmetics and relates to the discovery and application of anti-aging components, in particular to a plant-derived polypeptide with anti-aging effects, a face cream containing the same and a preparation method. Background technique [0002] Skin aging is a part of body aging, manifested as skin relaxation and thinning; the formation of wrinkles, which gradually increase and deepen, is one of the most intuitive external manifestations of body aging. Studies have shown that skin aging is related to the proliferation ability of skin fibroblasts and the decrease of dermal collagen content (Hu Xiaohui et al., Study on UVB-induced aging of human skin fibroblasts and its mechanism, Journal of Shanghai Jiaotong University, 2010). Therefore, promoting the proliferation of human skin fibroblasts and the synthesis of collagen is an important aspect to keep the skin young and resist aging. [0003] Polypeptide is a part of pep...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14C07K1/34A61K8/64A61K8/9789A61Q19/08
CPCC12P21/06C07K1/145C07K1/34A61K8/645A61K8/9789A61Q19/08A61K2800/805A61K2800/84
Inventor 不公告发明人
Owner 张超科
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