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Bionic modified valve material as well as preparation method and application thereof

A biological valve and valve technology, applied in the field of biomedical materials, can solve the problems of valve thrombosis, calcified mechanical properties, and shortened valve life, and achieve the effects of enhanced drug release, wide sources, and mass production

Active Publication Date: 2020-12-18
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the other hand, clinical data also showed that GLU-treated BHVs could inhibit the adhesion and proliferation of endothelial cells due to the high toxicity of GLU, and GLU-treated BHVs showed disadvantages such as immunogenic response, calcification, etc.
Therefore, GLU-treated BHVs lead to problems such as valve thrombosis, difficult endothelialization, severe calcification, and shortened valve life (usually only 10-15 years) due to altered mechanical properties and impaired function.

Method used

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  • Bionic modified valve material as well as preparation method and application thereof
  • Bionic modified valve material as well as preparation method and application thereof
  • Bionic modified valve material as well as preparation method and application thereof

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Embodiment 1

[0044] A preferred embodiment of the present invention provides a method for preparing a bionic modified valve material, specifically a method for preparing red blood cell membrane-wrapped nano drug-loaded particles for bionic modification of porcine pericardial valves, which is obtained by the following steps:

[0045] 1) Preparation of drug-loaded PLGA nanoparticles (RAPA&AC@PLGA)

[0046] PLGA (10 mg, MW.50000, 50:50), RAPA (1 mg), AC (1 mg) were dissolved in 1 mL of dimethylsulfoxide (DMSO). Then the mixed solution was added dropwise to 3 mL of water and stirred gently. After 2h, use a dialysis bag (MWCO=3500da) to dialyze for 48h to prepare drug-loaded nanoparticles, such as figure 1 .

[0047] 2) Preparation of erythrocyte membrane vesicles

[0048] Whole blood was centrifuged at 4°C (2000rpm, 10min) to remove serum. Red blood cells were washed with phosphate buffered saline (PBS, 3 times) pH=7.4. Red blood cells were then resuspended in 0.25×PBS (pH=7.4) containing...

Embodiment 2

[0054] A preferred embodiment of the present invention provides a method for preparing a biomimetic modified valve material, specifically a method for preparing platelet membrane-wrapped nano drug-loaded particles for bionic modification of porcine pericardial valves, which is obtained by the following steps:

[0055] 1) Preparation of drug-loaded nanoparticles

[0056] PLGA (10 mg, MW.50000, 50:50), a hydrophobic anti-inflammatory drug (1 mg) was dissolved in 1 mL of dimethyl sulfoxide (DMSO). Then the mixed solution was added dropwise to 3 mL of water and stirred gently. After 2 hours, use a dialysis bag (MWCO=3500da) to dialyze for 48 hours to prepare drug-loaded nanoparticles.

[0057] 2) Preparation of platelet membrane

[0058] First, whole blood was centrifuged at 1600 rpm for 20 minutes to obtain platelets. Platelets were washed with phosphate buffered saline (PBS, 3 times) pH=7.4. Platelets were then resuspended in 0.25×PBS (pH=7.4) containing EDTAK2 at 4°C. Afte...

Embodiment 3

[0064] A preferred embodiment of the present invention provides a method for preparing a biomimetic modified valve material, specifically a method for preparing macrophage membrane-wrapped nano drug-loaded particles for bionic modification of porcine pericardial valves, which is obtained by the following steps:

[0065] 1) Preparation of drug-loaded nanoparticles

[0066] PLGA (10 mg, MW.50000, 50:50), hydrophobic anticoagulant drug (1 mg) was dissolved in 1 mL dimethyl sulfoxide (DMSO). Then the mixed solution was added dropwise to 3 mL of water and stirred gently. After 2 hours, use a dialysis bag (MWCO=3500da) to dialyze for 48 hours to prepare drug-loaded nanoparticles.

[0067] 2) Preparation of macrophage membrane

[0068] Macrophages are first cultured and collected. Then, macrophages were resuspended in 0.25×PBS (pH=7.4) at 4°C. After 30 min, subcellular organelles such as macrophage nuclei were removed by gradient density centrifugation, and macrophage membranes w...

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Abstract

The invention discloses a bionic modified valve material as well as a preparation method and application thereof. The preparation method comprises the following steps: A, synthesizing drug-loaded nanoparticles for loading drugs; B, preparing cell membrane vesicles; C, mixing the drug-loaded nanoparticles with cell membrane vesicles, and performing extruding for multiple times to prepare drug-loaded nanoparticles wrapped with cell membranes; and D, crosslinking the drug-loaded nanoparticles wrapped with cell membranes to the surface of a bioprosthetic valve to obtain the bionic modified valve material. The method disclosed by the invention has the advantages of simplicity in operation, mild reaction conditions and the like, and the bionic modified valve material prepared of the drug-loadednanoparticles wrapped with cell membranes, prepared by the method disclosed by the invention can be used for well solving the problems of easiness in thrombus and calcification, endothelialization difficulty and the like of an existing glutaraldehyde cross-linked valve. Besides, a strategy of combining a cell membrane bionic drug delivery system with the valve provides a new generation of treatment mode for safe and efficient treatment of valvular heart diseases.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a bionic modified valve material and its preparation method and application. Background technique [0002] Valvular heart disease (VHD) currently affects more than 100 million people worldwide and primarily consists of valve stenosis or regurgitation. Degenerative valvular disease and rheumatic heart disease have become a growing problem among older populations in developing countries as the population ages. In recent years, the medical treatment for heart valve dysfunction is still being explored, and the lack of understanding of the pathophysiology and progression of VHD greatly limits the development of its medical treatment. In the case of severe valve dysfunction, replacing the damaged valve with an artificial valve (mainly including mechanical and biological valves) is the most effective solution. By 2050, more than 850,000 patients are expected t...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/54
CPCA61L27/3604A61L27/3687A61L27/54A61L2300/412A61L2300/42A61L2300/602A61L2300/622A61L2400/12A61L2430/20
Inventor 王云兵胡成罗日方
Owner SICHUAN UNIV
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