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Vitamin B2 extraction method

An extraction method, vitamin technology, applied in the direction of organic chemistry, etc., can solve the problems of long oxidation reaction time, poor safety of hydrogen peroxide, affecting yield and quality, etc. The effect of refining the extraction progress

Active Publication Date: 2020-12-25
NINGXIA KINGVIT PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] 1. During the alkaline dissolution process of Bacillus subtilis fermentation broth, under alkaline conditions, VB 2 Easy to decompose, such as long residence time, VB 2 Severely damaged, affecting yield and quality
[0011] 2. Oxidants such as permanganate, sodium nitrate, hydrogen peroxide, and air are used to reduce VB 2 Oxidation, there will be many problems, such as many oxidation and decomposition products of potassium permanganate, producing MnO 2 、K 2 MnO 4 , and potassium permanganate itself is a colored substance, which is easy to affect the quality of the product; sodium nitrate and air oxidation are poor, and the reduced VB 2 The oxidation reaction takes a long time; the safety of hydrogen peroxide is poor, and it will decompose rapidly after contacting with many inorganic compounds or impurities, resulting in explosion, and it is easy to corrode metal containers, damage equipment, and affect product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Take Bacillus subtilis fermentation liquid 20m respectively 3 And 5% (w / w) ammonia water, transported by pump, metered by flow meter, and mixed at the same time into a dosing pipeline mixer to detect and adjust pH8.5, into a disc centrifuge, and adjust the disc centrifuge The centrifuge speed is 2000rpm, the light liquid of the first-level disc centrifuge enters the light liquid collection tank, and the first-level slag liquid returns to the dilution tank; the first-level slag liquid is diluted with water to obtain a solid content of 8% (w / w) dilution, and 5% (w / w) ammonia water is transported by pump, measured by flow meter, mixed by secondary dosing pipeline mixer, adjusted to pH 11.0, fed into secondary disc centrifuge, and adjusted by secondary disc centrifuge Rotate at 5500rpm, collect primary and secondary centrifugal light liquid, and mix to obtain VB 2 Centrifuge light liquid. The filtration yield was 96.6%.

[0031] Put the above VB 2 Centrifuge the light l...

Embodiment 2

[0034] Take Bacillus subtilis fermentation liquid 20m respectively 3 and 6% (w / w) ammonia water, transported by pump, metered by flow meter, and mixed in a dosing pipeline mixer at the same time, detect and adjust pH9.0, enter a disc centrifuge, adjust the disc centrifuge The speed of the centrifuge is 2500rpm, the light liquid of the first-level disc centrifuge enters the light liquid collection tank, and the first-level slag liquid returns to the dilution tank; the first-level slag liquid is diluted with water to obtain a solid content of 10% (w / w) dilution, and 6% (w / w) ammonia water is transported by pump, measured by flow meter, mixed by secondary dosing pipeline mixer, adjusted to pH 12.0, fed into secondary disc centrifuge, and adjusted by secondary disc centrifuge Rotate at 6000rpm, collect primary and secondary centrifuged light liquid, and mix to obtain VB 2 Centrifuge light liquid. The filtration yield is 97.5%.

[0035] Put the above VB 2 Centrifuge the light l...

Embodiment 3

[0038] Take Bacillus subtilis fermentation liquid 20m respectively 3 And 7% (w / w) ammonia water, transported by pump, metered by flow meter, and mixed at the same time into a dosing pipeline mixer to detect and adjust pH9.5, into a disc centrifuge, and adjust the disc centrifuge The centrifuge speed is 3000rpm, the light liquid of the first-level disc centrifuge enters the light liquid collection tank, and the first-level slag liquid returns to the dilution tank; the first-level slag liquid is diluted with water to obtain a solid content of 12% (w / w) dilution, and 7% (w / w) ammonia water is transported by pump, measured by flow meter, mixed by secondary dosing pipeline mixer, adjusted to pH 11.8, fed into secondary disc centrifuge, and adjusted by secondary disc centrifuge Rotate at 6500rpm, collect primary and secondary centrifuged light liquid, and mix to obtain VB 2 Centrifuge light liquid. The filtration yield was 98.2%.

[0039] Put the above VB 2 Centrifuge the light ...

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Abstract

The invention relates to a vitamin B2 extraction method, which adopts ammonia water two-stage alkalization dissolution and disc centrifuge centrifugal separation to extract vitamin B2 from bacillus subtilis fermentation broth, shortens the alkalization time, reduces the VB2 decomposition rate under alkaline conditions, and improves the alkalization filtration yield and quality. Meanwhile, low-concentration hypochlorous acid is adopted as an oxidizing agent, the oxidation effect on reduced VB2 is high, hypochlorous acid also has a bleaching effect at the same time, and the quality of VB2 is improved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation extraction, in particular to a vitamin B 2 Extraction Method. Background technique [0002] Vitamin B 2 (VB 2 ), also known as riboflavin, chemical name: 7,8-dimethyl-10-(1 ， -D-ribosyl)-isoalloxazine. VB 2 It is a part of the prosthetic group of yellow enzymes in humans and animals (yellow enzymes play the role of hydrogen transfer in biological redox), is one of the indispensable vitamins in biological life activities, and has a close relationship with the metabolism of carbohydrates, fats and amino acids Relationships play an important role in the growth and development of organisms. VB cannot be synthesized in humans and animals 2 , must be supplied by food. When lack of VB 2 When it is high, it will affect the biological oxidation of the body and cause metabolic disorders. The lesions are mostly manifested as inflammation of the mouth, eyes and external genitalia, su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D475/14
CPCC07D475/14
Inventor 裴立忠户鹏东张江飞高宏伟郭静沈毅丁永财
Owner NINGXIA KINGVIT PHARMA