A kind of multi-antigen epitope protein of chicken infectious bursal disease virus and its application

Abstract

The invention provides a chicken infectious bursal disease virus (IBDV) multi-antigen epitope protein and its application. The amino acid sequence of the protein is shown in SEQ ID NO.1. This application uses chicken immunoglobulin IgG The constant region of the heavy chain (γCH) is used as the carrier protein of the multi-epitope fusion protein, and the B-cell epitope and T-cell epitope genes on the representative strains of IBDV VP2 and VP3 are tandemly synthesized and connected to the γCH-encoding gene. Construction of recombinant plasmid pET‑VP 2+3 ‑γCH, and transformed into Escherichia coli BL21 for expression. Recombinant multi-antigen epitope fusion protein pVP 2+3 After purification and identification of ‑γCH, the immune challenge protection test of SPF chickens initially showed a good immune protection effect. This study successfully constructed and expressed recombinant multi-antigen epitope protein pVP 2+3 ‑γCH lays the foundation for the development of IBDV multi-epitope vaccines.

Description

technical field [0001] The invention relates to a chicken infectious bursal disease virus multi-antigen epitope protein and application thereof, belonging to the field of biotechnology. Background technique [0002] At present, the clinically used livestock and poultry virus disease vaccines are mainly attenuated vaccines and inactivated vaccines. In the clinical application of the attenuated vaccine, there may be safety problems such as strain reversion and gene recombination between the vaccine strain and the wild-type strain. The inactivated vaccine tends to induce humoral immunity, and its ability to induce cellular immunity is relatively weak. An ideal vaccine should not only be safe, but also induce the body's specific humoral immunity and cellular immunity at the same time. By inducing humoral immunity to produce high-affinity neutralizing antibodies, preventing the virus from binding to target cell receptors. Inducing cellular immunity produces specific cytotoxic ...

AI Technical Summary

Problems solved by technology

However, it is worth noting that although conventional vaccines can suppress the occurrence of the disease to a certain extent, there are now some reports of immunization failures, and the outbreak of the disease occurred in chicken farms vaccinated with IBDV vaccines due to immunization failures.

There are many reasons for the failure of immunization, and the difference between the virus itself and the vaccine strain directly leads to the inability of the antibodies produced by immunization to resist the attack of the virus, which is the main reason for the failure of immunization

IBDV is prone to mutation, and many reports of immunization failures indicate that research on new vaccines is urgent

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