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A cr(ⅵ)-reducing strain c6 and its culture conditions and application

A strain, Bacillus technology, applied to Cr-reducing strain C6 and its culture conditions and application fields, can solve problems such as poor environmental adaptability, large inoculum amount, and long time required for reduction

Active Publication Date: 2022-03-01
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Most of the existing microorganisms that can reduce Cr(Ⅵ) are characterized by low Cr(Ⅵ) tolerance, large inoculation amount, high cultivation cost, low reduction amount, long reduction time, poor environmental adaptability and harmful to human body. problem, the present invention provides a Cr(VI) reducing strain C6 and its culture conditions and application

Method used

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  • A cr(ⅵ)-reducing strain c6 and its culture conditions and application
  • A cr(ⅵ)-reducing strain c6 and its culture conditions and application
  • A cr(ⅵ)-reducing strain c6 and its culture conditions and application

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Embodiment 1

[0029] 1. Materials and methods

[0030] 1.1 Materials

[0031] 1.1.1 Source of bacteria

[0032] In September 2018, at the Chromium Salt Factory in Luancheng District, Shijiazhuang, a sampling shovel was used to collect 1.0 cm of surface soil from the heavily polluted surface, remove stones and other debris in the soil, and put about 200 g into a sterile sampling bag. Store at -20°C for later use.

[0033] 1.1.2 Reagents and instruments

[0034] Na 2 CrO 4 , tryptone, yeast powder, agar, NaCl and glycerol.

[0035] Electronic balance, Changshu Shuangjie Testing Instrument Factory;

[0036] Analytical balance, Mettler-Toledo (Shanghai) Co., Ltd.;

[0037] Electronic pH meter, Ohaus (Shanghai) Co., Ltd.;

[0038] Magnetic stirrer, Ronghua Instrument Company, Jintan City, Jiangsu Province;

[0039] Pipette, Dalong Medical Equipment Co., Ltd.;

[0040] Refrigerator, Haier Company;

[0041] Ultra-low temperature freezer storage box, Zhongke Meiling;

[0042] Single-per...

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Abstract

The invention relates to the technical field of microbial application, and specifically discloses a Cr(VI)-reducing bacterial strain C6, its culture conditions and application. The strain preservation number of the Cr(VI)-reducing strain C6 is CGMCC No.20390, which can be used in the treatment of Cr(VI)-contaminated wastewater and Cr(VI)-contaminated soil, as well as in the preparation of Cr(VI)-contaminated wastewater treatment agents or Cr(Ⅵ) polluted soil treatment agent. The Cr(VI)-reducing strain C6 provided by the present invention has both high-concentration Cr(VI) resistance and the ability to quickly and efficiently reduce Cr(VI), and the Cr(VI)-reducing strain C6 has strong environmental adaptability and good environmental safety , No pathogenicity to human body, low culture cost, small inoculum amount in Cr(Ⅵ) reduction, no secondary pollution, and high industrial promotion and application value.

Description

technical field [0001] The invention relates to the technical field of microorganism application, in particular to a Cr(VI)-reducing bacterial strain C6 and its culture conditions and application. Background technique [0002] Chromium (Cr) has been widely used in industries such as leather, electroplating, pigments, mineral processing, chemicals and wood preservation since it was discovered by French scientist Vauquelin from chromium lead ore in 1797, and has made great contributions to industrial development. At the same time, the mining, smelting, processing and commercial manufacturing activities of chromium and chromium salts as raw materials are increasing day by day, resulting in a large amount of chromium-containing waste residue and wastewater. Chromium exists in nine valence states under natural conditions, but the most common chromium compounds in the atmosphere, soil and water are in the form of hexavalent chromium Cr(VI) or trivalent chromium Cr(III). Cr(Ⅲ) is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/07C02F101/22
CPCC12N1/20B09C1/10C02F3/34C02F2101/22C12R2001/07C12N1/205
Inventor 谷雅文周磊景任玉文于鲲鹏周晓辉
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY