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Dental pulp mesenchymal stem cell cryopreservation liquid and cryopreservation method

A technique of stem cells and cryopreservation method, which is applied in the field of dental pulp mesenchymal stem cell cryopreservation solution, can solve the problems of unable to guarantee the vitality of stem cells, affecting the clinical utilization of stem cells, and damage of dental pulp stem cells, so as to avoid adverse effects and avoid apoptosis. death and avoid structural damage

Active Publication Date: 2021-01-05
北京泰盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current cryopreservation solutions will inevitably cause some damage to dental pulp stem cells during the cryopreservation process of cells, cannot guarantee the vitality of stem cells, and the cell proliferation rate is low, which affects the clinical utilization of stem cells

Method used

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  • Dental pulp mesenchymal stem cell cryopreservation liquid and cryopreservation method

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Experimental program
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Embodiment 1

[0026] A dental pulp mesenchymal stem cell cryopreservation solution, consisting of component A and component B, component A is a serum-free medium DMEM / F12 medium, component B is composed of the following raw materials: titanate nanotubes, 5 - disodium adenosine phosphate, vitamin E, 1,8-cineole, glycerol, the concentration of each raw material in the cryopreservation solution is: titanate nanotubes 6.5 μg / mL, disodium adenosine 5-phosphate 7.8 μg / mL mL, vitamin E1.5μg / mL, 1,8-cineole 10.2mg / mL, glycerin 25mg / mL;

[0027] Among them, the diameter of the titanate nanotube is 4-10nm, the length is 100-500nm, and the specific surface area is 200-300m 2 / g.

[0028] A cryopreservation method for dental pulp mesenchymal stem cells, comprising the following steps:

[0029] (1) The P3 generation dental pulp mesenchymal stem cells were resuspended in the serum-free medium of component A, and the cell density after resuspension was 2×10 6 pieces / mL, pipette evenly, and cool down to...

Embodiment 2

[0032] A dental pulp mesenchymal stem cell cryopreservation solution, consisting of component A and component B, component A is a serum-free medium DMEM / F12 medium, component B is composed of the following raw materials: titanate nanotubes, 5 - disodium adenosine phosphate, vitamin E, 1,8-cineole, glycerol, the concentration of each raw material in the freezing solution is: titanate nanotubes 8.5 μg / mL, disodium adenosine 5-phosphate 8.0 μg / mL mL, vitamin E3.8μg / mL, 1,8-cineole 12.9mg / mL, glycerin 28mg / mL;

[0033] Among them, the diameter of the titanate nanotube is 4-10nm, the length is 100-500nm, and the specific surface area is 200-300m 2 / g.

[0034] A cryopreservation method for dental pulp mesenchymal stem cells, comprising the following steps:

[0035] (1) The P4 dental pulp mesenchymal stem cells were resuspended in the serum-free medium of component A, and the cell density after resuspension was 3×10 6 pcs / mL, blow and beat evenly, cool down to 6°C;

[0036] (2) ...

Embodiment 3

[0038] A dental pulp mesenchymal stem cell cryopreservation solution, consisting of component A and component B, component A is a serum-free medium DMEM / F12 medium, component B is composed of the following raw materials: titanate nanotubes, 5 - disodium adenosine phosphate, vitamin E, 1,8-cineole, glycerol, the concentration of each raw material in the cryopreservation solution is: titanate nanotubes 9.2 μg / mL, disodium adenosine 5-phosphate 8.5 μg / mL mL, vitamin E4.5μg / mL, 1,8-cineole 14.5mg / mL, glycerin 30mg / mL;

[0039] Among them, the diameter of the titanate nanotube is 4-10nm, the length is 100-500nm, and the specific surface area is 200-300m 2 / g.

[0040] A cryopreservation method for dental pulp mesenchymal stem cells, comprising the following steps:

[0041] (1) The P5 dental pulp mesenchymal stem cells were resuspended in the serum-free medium of component A, and the cell density after resuspension was 4×10 6 pcs / mL, blow evenly, cool down to 4-6°C;

[0042] (2)...

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Abstract

The invention discloses a dental pulp mesenchymal stem cell cryopreservation liquid which comprises a component A and a component B. The component A is a serum-free culture medium, and the component Bis composed of the following raw materials: titanic acid nanotubes, disodium adenosine 5-phosphate, vitamin E, 1, 8-cineole and glycerin. No animal serum is added into the cryopreservation liquid, sothat exogenous pollution possibly caused by the animal serum is avoided. 1, 8-cineole and glycerol in the component B have a synergistic effect, so that moisture in cells is prevented from crystallizing when the cells are close to a freezing point, and structural damage in the cells is avoided. Due to the added titanic acid nanotube, disodium adenosine 5-phosphate and vitamin E, on one hand, thecells keep certain activity in the cryopreservation process in liquid nitrogen, and apoptosis of the cells is avoided; on the other hand, after cryopreservation is finished, cells can be quickly recovered, and the activity can be recovered. The invention also provides a dental pulp mesenchymal stem cell cryopreservation method, and the dental pulp mesenchymal stem cell cryopreservation liquid provided by the invention is adopted in the method, no complex programmed cooling is needed, and operation is simple.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a cryopreservation solution and a cryopreservation method for dental pulp mesenchymal stem cells. Background technique [0002] Dental pulp mesenchymal stem cells (DPSCs) are a kind of mesenchymal stem cells with high proliferative ability and multi-lineage differentiation potential. The medical field has made great progress and has broad application prospects. At the same time, DPSCs can be induced to differentiate into bone tissue, cartilage tissue, adipose tissue, nerve tissue, muscle tissue, cornea and other tissue cells under appropriate conditions, and can be induced into induced pluripotent stem cells with characteristics similar to embryonic stem cells. It can be used as seed cells in the clinical transformation research of various diseases in the future. At present, many countries have established dental pulp stem cell banks for clinical treatment or scientific research needs...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 杨莹
Owner 北京泰盛生物科技有限公司
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