Plant lactobacillus solid inoculant, preparation method thereof and application of plant lactobacillus solid inoculant

A technology of plant lactic acid bacteria and solid microbial agents, applied in biochemical equipment and methods, methods based on microorganisms, applications, etc., can solve the problems of low bacterial count, low metabolite lactic acid content, poor fermentation effect, etc.

Active Publication Date: 2021-01-05
COFCO BIOTECHNOLOGY CO LTD +1
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the solid fermentation of plant lactic acid bacteria, the strains of plant lactic acid bacteria are usually inoculated into the solid medium for fermentation and culture, but the cur...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plant lactobacillus solid inoculant, preparation method thereof and application of plant lactobacillus solid inoculant
  • Plant lactobacillus solid inoculant, preparation method thereof and application of plant lactobacillus solid inoculant
  • Plant lactobacillus solid inoculant, preparation method thereof and application of plant lactobacillus solid inoculant

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] First aspect, the present invention provides a kind of preparation method of plant lactic acid bacteria solid microbial agent, the method comprises:

[0021] (1) inoculating the strains of plant lactic acid bacteria into the first solid fermentation medium to carry out the first fermentation culture to obtain the first fermentation material;

[0022] (2) inoculating the first fermentation material into a second solid fermentation medium to carry out a second fermentation culture to obtain a second fermentation material;

[0023] Wherein, the first solid fermentation medium contains starchy raw material crushed product and starch residue;

[0024] The second solid fermentation medium contains DDGS, DDS, starchy raw material skin and calcium source.

[0025] According to the present invention, although the strains of plant lactic acid bacteria are inoculated into the above specific solid fermentation medium for secondary culture, the lactic acid content and the cell dens...

preparation example 1

[0067] This preparation example is used to illustrate the preparation of the bacterial classification of plant lactic acid bacteria

[0068] According to the medium and conditions in Table 1, the activation and expansion of plant lactic acid bacteria were carried out, and the fermentation liquid was obtained as the inoculum with the expansion tank.

[0069] Table 1

[0070]

preparation example 2

[0072] This preparation is used to illustrate the activation of Saccharomyces cerevisiae inoculum

[0073] Activate the yeast agent with 7 times of 2% by weight aqueous glucose solution at 30-35°C for 15-30 minutes to obtain activated yeast

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of microbial fermentation feed, in particular to a plant lactobacillus solid inoculant, a preparation method thereof and application of the plant lactobacillus solidinoculant. The method comprises the following steps: (1) inoculating a strain of plant lactobacillus into a first solid fermentation medium for first fermentation culture to obtain a first fermentation material; and (2) inoculating the first fermentation material into a second solid fermentation medium for second fermentation culture to obtain a second fermentation material; wherein the first solid fermentation medium contains a starchy raw material crushed product and starch dregs; and the second solid fermentation medium contains DDGS, DDS, starchy raw material bran and a calcium source. According to the technical scheme, two-time fermentation is performed on the plant lactobacillus in a specific fermentation medium, so that the metabolic capability of the strain is remarkably improved,and the lactic acid content and number of thalli in a finally obtained fermentation product are greatly increased, namely the fermentation effect is improved.

Description

technical field [0001] The invention relates to the field of microbial fermented feed, in particular to a solid bacterial agent of plant lactic acid bacteria and a preparation method and application thereof. Background technique [0002] Microbial fermented feed has natural fermented flavor and good food-attracting effect. Plant lactic acid bacteria, such as plant Lactobacillus, are the main probiotics in the intestinal tract of humans and animals. They have many health care functions, such as maintaining the balance of intestinal flora, regulating immunity, and promoting nutrient absorption; they have certain effects on pathogenic bacteria. Inhibitory effect, improving intestinal microenvironment; alleviating lactose intolerance. In addition, plant lactic acid bacteria are a type of microorganisms that normally exist in the digestive tract, and have strong tolerance and colonization ability in the intestinal mucosa. [0003] With the popularization of the application rang...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A23K10/18C12R1/25
CPCC12N1/20A23K10/18Y02P60/87
Inventor 佟毅张琳刘利利卢宗梅王迪领杨鑫金渭武李义
Owner COFCO BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap