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Lung stem cell with antibacterial ability and application thereof

A stem cell and lung technology, applied in the field of stem cell transplantation, can solve the problems of inability to inhibit and weaken the treatment effect of stem cells in patients with bacterial infection, so as to avoid side effects.

Pending Publication Date: 2021-01-05
TONGJI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, stem cells cannot directly inhibit bacterial infections, which weakens the therapeutic effect of stem cells on patients with bacterial infections
[0004] At present, humans use antibiotics and synthetic antibacterial drugs to treat bacterial infectious diseases, but the drug resistance and drug resistance caused by the irrational use of antibiotics and synthetic antibacterial drugs in clinical practice has become a fact that cannot be ignored

Method used

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  • Lung stem cell with antibacterial ability and application thereof
  • Lung stem cell with antibacterial ability and application thereof
  • Lung stem cell with antibacterial ability and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The human lung stem cells prepared in this example contain a recombinant vector expressing the antimicrobial peptide LL-37. The expression framework of the antimicrobial peptide LL-37 is: the promoter, the gene encoding the signal peptide, and the antimicrobial peptide LL-37 in the 5' to 3' direction. 37 encoding genes. The preparation method is as follows:

[0045] Isolation and Characterization of Human Lung Stem Cells:

[0046] The isolated active bronchial brushing tissue was taken for later use.

[0047] Take the tissue digestion solution and stop solution for later use; 99v% of the tissue digestion solution is DMEM / F12, and the rest are 1-20ng / mL DNase, 0.1-4mg / mL protease and 10-200ng / mL trypsin; stop solution 90v% is DMEM, 10v% is FBS.

[0048] The above-mentioned brushed tissue is digested with the tissue digestion solution, and the digested tissue is terminated with the stop solution, and then the cells are collected.

[0049] Take the medium for use, in w...

Embodiment 2

[0058] The human lung stem cells prepared in this example contain a recombinant vector expressing the antimicrobial peptide hCAP18. The expression framework of the antimicrobial peptide hCAP18 is: the promoter in the 5' to 3' direction, followed by the gene encoding the antimicrobial peptide hCAP18. The preparation method is as follows:

[0059] Isolation and Characterization of Human Lung Stem Cells:

[0060] The isolated active bronchial brushing tissue was taken for later use.

[0061] Take the tissue digestion solution and stop solution for later use; 99v% of the tissue digestion solution is DMEM / F12, the rest is 1-20ng / mL DNase, 0.1-4mg / mL protease XIV and 10-200ng / mL trypsin; stop 90v% of the liquid is DMEM, and 10v% is FBS.

[0062] The above-mentioned brushed tissue is digested with the tissue digestion solution, and the digested tissue is terminated with the stop solution, and then the cells are collected.

[0063] Take the medium for later use, including 225mL DME...

experiment example 1

[0072] The mouse lung stem cells prepared in this example contain a recombinant vector expressing the antimicrobial peptide hCAP18. The expression framework of the antimicrobial peptide hCAP18 is: the promoter and the gene encoding the antimicrobial peptide hCAP18 in the 5' to 3' direction.

[0073] Isolation and Characterization of Mouse Lung Stem Cells:

[0074] The lungs of adult C57 mice were taken out with sterile forceps and soaked in F12 medium containing 0.5-1.5v% Pen / Strep, 3-10v% FBS. Discard the lung main bronchi and separate the lung lobes into small pieces using a sterile scalpel.

[0075] Take the tissue digestion solution and stop solution for later use; 99v% of the tissue digestion solution is DMEM / F12, and the rest are 1-20ng / mL DNase, 0.1-4mg / mL protease and 10-200ng / mL trypsin; stop solution 90v% is DMEM, 10v% is FBS.

[0076]Use tissue digestion solution to digest the above small pieces of lung lobe overnight. After the digestion is terminated, the cells ...

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Abstract

The invention discloses a lung stem cell with antibacterial ability and application thereof, and belongs to the technical field of stem cell transplantation. The invention provides the lung stem cellwith antibacterial ability. The lung stem cell contains a recombinant vector for expressing antibacterial peptide hCAP18 / LL37. The lung stem cell has injury repair and antibacterial functions, can repair injured tissues on one hand, and can prevent and relieve microbial infection in the body of a patient on the other hand. The lung stem cell provided by the invention can be remained in vivo for along time, so that the antibacterial effect can be maintained for a long time. The lung stem cell with antibacterial ability provided by the invention can be specifically distributed in the lung, andachieves the antibacterial effect in the lung.

Description

technical field [0001] The invention relates to the technical field of stem cell transplantation, in particular to a lung stem cell with antibacterial ability and its application. Background technique [0002] There are a large number of normal flora on the surface of the human body and the cavity that communicates with the outside world. They are beneficial and harmless to the human body when the immune function of the human body is normal; When it decreases, the original normal resident bacteria or exogenous bacteria may invade the human body, causing local or systemic inflammatory reactions in the human body, and subsequently causing destructive damage to some tissues and cells in the body. For patients with tissue damage lesions, microbial infections such as bacteria and fungi often coexist with tissue damage. On the one hand, it is easy to cause various complications, and on the other hand, it will continue to aggravate tissue damage and cause substantial damage to soli...

Claims

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Application Information

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IPC IPC(8): C12N5/10C12N15/867C12N15/12C12N5/071A61K35/42A61P11/00A61P31/00
CPCC07K14/4723C12N5/0689C12N15/86A61K35/42A61P11/00A61P31/00C12N2510/00C12N2500/32C12N2501/33C12N2501/11C12N2500/40C12N2501/39C12N2740/15043
Inventor 左为张婷马煜周悦青
Owner TONGJI UNIV
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