The invention discloses a preparation method of silk
fibroin nanofibers and a preparation method of a silver-loaded antibacterial dressing based on the silk
fibroin nanofibers. The preparation methodof the silk
fibroin nanofibers comprises the following steps: 1) putting silkworm cocoons into a
sodium carbonate solution, and performing degumming to obtain silk fibroin; 2) dissolving the silk fibroin in a
sodium hydroxide /
urea aqueous solution to obtain a dissolved solution; 3) putting a
dialysis bag into a
sodium bicarbonate / EDTA solution, performing boiling treatment, taking out the dialysisbag, putting the
dialysis bag into an EDTA solution, and performing boiling treatment; and 4) pouring the solution obtained in the step 2) into the treated
dialysis bag, performing dialysis, performing ultrasonic treatment, and performing freeze
centrifugation to obtain the silk fibroin
nanofiber aqueous solution. The invention also discloses the preparation method of the silver-loaded antibacterial dressing, and the method comprises the following steps: 1) preparing the silk fibroin
nanofiber aqueous solution by using the above method; 2) dissolving
silver nitrate in the obtained silk fibroin
nanofiber aqueous solution, wherein the
mass-to-volume ratio of the
silver nitrate to the silk fibroin nanofiber aqueous solution is 0.02%-0.08%, and performing
irradiation from the cup bottom underultraviolet conditions; and 3) after the
ultraviolet irradiation is completed, performing pre-cooling, performing freezing, and finally performing vacuum
freeze drying to obtain the silver-loaded antibacterial dressing.