Preparation method of silk fibroin nanofibers and preparation method of silver-loaded antibacterial dressing based on silk fibroin nanofibers

Abstract

The invention discloses a preparation method of silk fibroin nanofibers and a preparation method of a silver-loaded antibacterial dressing based on the silk fibroin nanofibers. The preparation methodof the silk fibroin nanofibers comprises the following steps: 1) putting silkworm cocoons into a sodium carbonate solution, and performing degumming to obtain silk fibroin; 2) dissolving the silk fibroin in a sodium hydroxide/urea aqueous solution to obtain a dissolved solution; 3) putting a dialysis bag into a sodium bicarbonate/EDTA solution, performing boiling treatment, taking out the dialysisbag, putting the dialysis bag into an EDTA solution, and performing boiling treatment; and 4) pouring the solution obtained in the step 2) into the treated dialysis bag, performing dialysis, performing ultrasonic treatment, and performing freeze centrifugation to obtain the silk fibroin nanofiber aqueous solution. The invention also discloses the preparation method of the silver-loaded antibacterial dressing, and the method comprises the following steps: 1) preparing the silk fibroin nanofiber aqueous solution by using the above method; 2) dissolving silver nitrate in the obtained silk fibroin nanofiber aqueous solution, wherein the mass-to-volume ratio of the silver nitrate to the silk fibroin nanofiber aqueous solution is 0.02%-0.08%, and performing irradiation from the cup bottom underultraviolet conditions; and 3) after the ultraviolet irradiation is completed, performing pre-cooling, performing freezing, and finally performing vacuum freeze drying to obtain the silver-loaded antibacterial dressing.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a preparation method of silk nanofibers and silver-loaded antibacterial dressings based on silk nanofibers. Background technique [0002] Silk fibroin (SF for short) is a natural, biocompatible, biodegradable and low-cost polymer fibrin extracted from silk. The SF in silkworm cocoons is composed of glycine, alanine, serine, tyrosine and valine with 5263 amino acid residues, while the other 15 amino acids only account for 4.7%. Due to their tunable mechanical properties and favorable physical structure, silk proteins are attractive for many biomedical applications, including drug delivery, tissue engineering, implantable devices, biosensors, and wound dressings. [0003] As the largest organ in the human body, the skin not only protects our normal metabolism, but also plays an important role in protecting us from UV radiation, chemicals, foreign organisms and oth...

AI Technical Summary

Problems solved by technology

However, this method has the following disadvantages: dissolution will over-shear silk fibroin and cut off most of the original hydrogen bonds, making the regenerated silk fibroin weaker than the original silk fibroin

This method only destroys a small amount of hydrogen bonds, so it can greatly protect the fiber structure and performance of natural silk fibroin, but its disadvantages are that it cannot maintain an aqueous solution in the formic acid / calcium chloride system, and adding water will cause protein precipitation; In the formic acid / lithium bromide system, the obtained aqueous solution must be stored at 4°C to avoid premature gelation; and HFIP is a toxic reagent

To sum up, traditional extraction methods have different limitations in different aspects, so a new type of extraction method is needed

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