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Biocontrol bacillus velezensis ZHX-7 and application thereof

A technology of ZHX-7 and Bacillus, applied in the fields of application, bacteria, biocide, etc., can solve serious diseases, threaten the health of the ecological environment and food safety, affect the healthy development of the peanut industry, etc., and achieve the effect of promoting growth and yield

Active Publication Date: 2021-01-08
SHANDONG PEANUT RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, in my country, due to repeated cropping and the lack of disease-resistant varieties, pathogenic bacteria have accumulated year after year, resulting in more serious diseases. Limited by the current peanut industry structure and farming system, chemical control is still the main method for the prevention and control of peanut underground diseases and insect pests. However, excessive reliance on chemical pesticides has resulted in soil, agricultural products, air, water quality, etc. being polluted to varying degrees, seriously affecting the healthy development of the peanut industry. Effective measures run counter to

Method used

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  • Biocontrol bacillus velezensis ZHX-7 and application thereof
  • Biocontrol bacillus velezensis ZHX-7 and application thereof
  • Biocontrol bacillus velezensis ZHX-7 and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Isolation and Identification of Bacillus Velez ZHX-7

[0030] 1. Screening and isolation of strain ZHX-7:

[0031] The samples used were the soil at a depth of 6 cm from the surface around the rhizosphere of diseased and healthy plants in peanut continuous cropping fields, and were stored at 4°C.

[0032] The steps include: (1) Weigh 10 g of the above soil sample and add it to 90 mL of sterile water, shake at 28 ° C and 140 rpm for 15 min, let it stand for 5 min, take the supernatant, and use the 10-fold serial dilution method to successively dilute to 10 -2 、10 -3 、10 -4 and 10 -5 100 μL of each concentration was applied to LB solid medium, each concentration was repeated 3 times, and cultured in the dark at 28°C. (2) When bacterial colonies appear on the culture medium, spray the peanut crown rot fungus (Aspergillus niger) spore suspension. After culturing for 2 days, select the colony with obvious inhibition zone around it, pick a single colony with a transfer r...

Embodiment 2

[0038] Inhibitory Effect of Strain ZHX-7 on Mycelia Growth of Peanut Pathogen

[0039] 1. Inhibitory effect of the strain ZHX-7 bacterial suspension on the mycelial growth of three kinds of peanut pathogens

[0040] (1) Peanut crown rot bacteria: Pick a single colony of strain ZHX-7 with a toothpick and put it into a conical flask filled with 20mL of LB medium, culture it with shaking at 28°C for 14h, and prepare a concentration of OD 420 =0.3 bacterial suspension. Take 10 μL of the bacterial suspension and inoculate it in the center of a PDA solid medium with a diameter of 9 cm. After the bacterial solution is fully absorbed, incubate at a constant temperature of 28°C for 24 hours. Spray OD evenly into PDA medium 420 =0.3 of the spore suspension of peanut crown rot fungus, with no spray spore suspension as contrast. Cultivate at a constant temperature of 25°C for about 4 days, count and take pictures of the size of the inhibition zone (see image 3 A, B). image 3 A is the...

Embodiment 3

[0053] Pot experiment on the prevention and treatment of peanut crown rot with bacterial fermentation liquid of strain ZHX-7

[0054] Bacterial solution of strain ZHX-7 (OD 420 =0.3) After soaking for 4 hours, a single seed was sown in a 320 mL disposable plastic cup, and 2 mL of fermentation broth was inoculated at the same time. Soak the LB medium and inoculate the same amount of LB medium as the blank control, and use 50% carbendazim 800 times water as the treatment control. At the same time, the peanut crown rot fungus was cultivated on the PDA plate. After 6 days, the spore suspension of the peanut crown rot fungus was prepared with sterilized water, and 5 mL was added to 150 mL of PDB, and cultured with shaking at 25 ° C for 24 h, used to inoculate peanuts. Each peanut plant was inoculated with 2 mL of the spore suspension of Peanut crown rot fungus, and the same amount of PDB was used as a control. 30 pots of control and treatment were repeated 3 times, and the mortal...

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Abstract

The invention belongs to the field of strains and application thereof, and particularly relates to a biocontrol bacillus velezensis ZHX7 with the preservation number of CGMCC No. 20374. The bacillus velezensis ZHX7 has an obvious inhibiting effect on peanut pathogenic bacteria and can be used as a potential biocontrol strain, the strain ZHX7 is applied to prevention and treatment of peanut diseases, the resistance of peanuts to crown rot, southern blight, net blotch bacteria, alternaria leaf spot bacteria, root rot bacteria and peanut banded sclerotial blight bacteria is improved, and meanwhile, the growth and the yield of the peanuts can be remarkably promoted. Tests prove that the strain ZHX7 can also inhibit aspergillus flavus of the peanuts and the toxin content of aspergillus flavus.The application method of the strain ZHX7 belongs to the field of biological prevention and control, is safe, rapid and effective, is beneficial to green and healthy production of the peanuts, and provides technical support for comprehensive prevention and control of peanut diseases.

Description

technical field [0001] The invention belongs to the field of biological technology, in particular to the field of strains and their applications, in particular to the isolation and identification of Bacillus velaisi which has broad-spectrum resistance to various plant pathogenic bacteria and its role in promoting the growth of peanuts and preventing and controlling peanut pathogenic fungi on the application. Background technique [0002] Peanut is an important oil crop and economic crop in my country, and it is one of the crops commonly planted in China. However, with the increase of peanut disease year by year, it has become the main obstacle for the further development of peanut in our country. Due to the wide variety of soil-borne pathogenic bacteria of peanuts and the different types of pathogenic bacteria in different regions, the disease has always been a difficult point in peanut production, especially in recent years, peanut crown rot, peanut white silkworm and pean...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00A23L5/20C12R1/07
CPCA01N63/22A23L5/28C12R2001/07C12N1/205
Inventor 张霞郭志青迟玉成许曼琳于静李莹宋新颖何康
Owner SHANDONG PEANUT RES INST
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