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Biocontrol bacillus velezensis ZHX-12 and application thereof

A ZHX-12, Bacillus technology, applied in the application, bacteria, biocide and other directions to achieve the effect of promoting growth and yield

Active Publication Date: 2021-02-12
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, scientists at home and abroad have successively isolated a large number of Bacillus Velez from plant rhizosphere soil, culture ponds, deep sea, and Daqu, and found that many Bacillus Velez strains are effective in promoting plant growth, antagonizing pathogenic bacteria and liquor brewing. etc., have very important theoretical research and practical application value, but so far there is no research on the application of Bacillus velesii in the prevention and control of peanut diseases.

Method used

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  • Biocontrol bacillus velezensis ZHX-12 and application thereof
  • Biocontrol bacillus velezensis ZHX-12 and application thereof
  • Biocontrol bacillus velezensis ZHX-12 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The isolation and identification of embodiment 1 Veles bacillus ZHX-12

[0033] 1. Screening and isolation of strain ZHX-12:

[0034] The samples were taken from the soil at a depth of 6 cm from the surface around the rhizosphere of diseased and healthy plants in peanut continuous cropping fields, and were stored at 4°C.

[0035] Steps include:

[0036] (1) Weigh 10g of the above soil sample and add it to 90mL sterile water, shake at 28°C and 140rpm for 15min, let it stand for 5min, take the supernatant, and use the 10-fold serial dilution method to successively dilute to 10 -2 、10 -3 、10 -4 and 10 -5 100 μL of each concentration was drawn and spread on LB solid medium, each concentration was repeated 3 times, and cultured in the dark at 28°C.

[0037] (2) When bacterial colonies appear on the culture medium, spray the spore suspension of Aspergillus niger. After cultivating for 2 days, select the colonies with obvious antibacterial zones around them, pick single c...

Embodiment 2

[0043] The inhibitory effect of embodiment 2 Bacillus velei ZHX-12 on peanut crown rot fungus, net spot fungus and ring spot fungus hyphae growth

[0044] (1) Inhibitory effect of strain ZHX-12 bacterial suspension on the mycelium growth of three kinds of peanut pathogens

[0045] 1. Peanut crown rot bacteria: Pick a single colony of strain ZHX-12 with a toothpick and put it into a conical flask filled with 20mL of LB medium, shake and culture at 28°C for 14h, and prepare a concentration of OD 420 =0.3 bacterial suspension. Take 10 μL of bacterial suspension and inoculate it in the center of PDA solid medium with a diameter of 9 cm. After the bacterial solution is fully absorbed, incubate at 28°C for 24 hours. Spray OD evenly into PDA medium 420 =0.3 of the spore suspension of peanut crown rot fungus, with no spray spore suspension as contrast. Cultivate at a constant temperature of 25°C for about 8 days, count and take pictures of the size of the inhibition zone (see ima...

Embodiment 3

[0053] The bacteriostasis of embodiment 3 Beles bacillus ZHX-12 to peanut root rot fungus, peanut white silkworm and peanut sheath blight

[0054] 1. Peanut root rot bacteria (Fusarium solani and Fusarium oxysporum): the concentration of the prepared strain ZHX-12 is OD 420 =0.3 bacterial suspension, draw 10 μL, and inoculate in the center of PDA solid medium with a diameter of 9 cm. After the bacterial solution is fully absorbed, incubate at a constant temperature of 28°C for 24 hours. Spray OD evenly into PDA medium 420 = 0.3 of the spore suspensions of Fusarium solani and Fusarium oxysporum, with the spore suspension not sprayed as a control, cultured at a constant temperature of 25°C for about 3 days, the size of the inhibition zone was counted and photographed, see Image 6 A-D, where, Image 6 A and 6C are the control groups of Fusarium solani and Fusarium oxysporum respectively, Image 6 B and 6D are the antibacterial effects of strain ZHX-12 on Fusarium solani and F...

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Abstract

The invention provides a biocontrol bacillus velezensis ZHX-12, the preservation number of the biocontrol bacillus velezensis ZHX-12 is CGMCC No.20375, and the 16S rDNA sequence of the biocontrol bacillus velezensis ZHX-12 is shown as SEQ ID NO: 1; the gyrB gene sequence of the gene is as shown in SEQ ID NO: 2. The biocontrol bacillus velezensis ZHX-12 is applied to prevention and treatment of peanut crown rot germs, peanut net blotch germs, peanut ring spot germs, peanut root rot germs, peanut southern blight germs and peanut sheath blight germs, and can promote peanut growth and inhibit peanut aspergillus flavus and the toxin content of the peanut aspergillus flavus. The application method of the bacillus velezensis ZHX-12 belongs to the field of biological prevention and control, is safe, quick and effective, is beneficial to green and healthy production of peanuts, provides technical support for comprehensive prevention and control of peanut diseases, and can promote peanut growthand inhibit aspergillus flavus and toxin content of aspergillus flavus.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the field of strains and their applications, in particular to the isolation and identification of Bacillus Velez ZHX-12 which has broad-spectrum resistance to various plant pathogenic bacteria and its role in promoting the growth of peanuts and preventing and controlling peanuts. Application on pathogenic fungi. Background technique [0002] Peanut is one of the important sources of food, feed and edible oil in the world. Peanut is easy to be infected with diseases, especially soil-borne diseases caused by fungi, including peanut crown rot and white silkworm. Peanut crown rot is caused by Aspergillus niger harming the kernels, cotyledons and stem bases, and mainly infecting the stem bases. The disease can survive in infected peanut seeds and spread through the seeds, and the seed kernels that are wet during storage are susceptible to pathogen infection, and the seed carrier rate is ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00A23L5/20C12R1/07
CPCA01N63/22A23L5/28C12R2001/07C12N1/205
Inventor 张霞迟玉成许曼琳郭志青于静何康李莹宋新颖
Owner SHANDONG PEANUT RES INST
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