40 results about "Phymatotrichum omnivorum" patented technology

The invention relates to Bacillus pumilus NMCC 46, which is preserved in CGMCC with preservation date as November 2, 2009, and preservation number as CGMCC No.3378. The Bacillus pumilus NMCC 46 CGMCC No.3378 in the invention is a biological control bacillus separated from grass root surrounding soil near Namco Lake in Tibet, which can grow at 10 DEG C, is favorable for exerting growth promotion and biological control effects, can restrain soybean root pythium myriotylum and saccharomycetes, can boost growth of Arabidopsis and soybean, has a prevention and control effects on soybean root pythium myriotylum as high as 85%, can increase yield of soybean to 24%, and has good application prospect.

The invention belongs to the technical field of prevention and control of plants diseases and particularly relates to a trichoderma koningiopsis T5-1 bacterial strain and an application thereof in improvement of growth of panax notoginseng and prevention and control of root rot. The bacterial strain is named trichoderma koningiopsis, and is assigned the accession number of CGMCC No. 12779 in China General Microbiological Culture Collection Center. The trichoderma koningiopsis T5-1 bacterial strain has developed mycelia and fluffy and dense aerial mycelia, and is free of generation of pigments. The trichoderma koningiopsis T5-1 bacterial strain can improve growth of panax notoginseng and also can inhibit growth of root rot fungus. The bacterial strain can be hyper-parasitized on the root rot fungus, so that spore production of the root rot fungus is inhibited by means of volatile organic compounds from the bacterial strain in a sealed condition. The bacterial strain lays a good foundation for development and utilization of bio-bacterial fertilizers.

The invention discloses an anti-bacterial protein PBR1 as well as a preparation method and application thereof, and relates to the technical field of molecular biology and biological pesticides. The amino acid sequence of the anti-bacterial protein PBR1 is shown as SEQ ID NO.1. The anti-bacterial protein PBR1 has a superior effect on preventing and curing plant diseases such as cruciferous plasmodiophoromycetes, paddy rice magnaporthe oryzae, curvularia lunata, fusarium oxysporum f. sp. dianthi, fusarium oxysporum vasinfectum, konjac foot rot bacteria, panax notoginseng root rot pathogen, pomegranate wilt pathogen, gaeumannomyces gramini, cylindrosporium pomi brooks, alternaria alternata and/or botrytis cinerea. A pesticide taking the anti-bacterial protein PBR1 as an active ingredient is a novel environment-friendly biological pesticide, is safe to people and livestock, protects the environment, and has a favorable development prospect.

The invention relates to a panax notoginseng seedling protection film, and a preparation method and an application thereof. The film provided by the invention is a water-soluble gel prepared by using a water-soluble film-forming material, panax notoginseng polysaccharide, a root growth promoter, a preservative, and a pesticide. With the material, a protection film can be formed on the surface of a panax notoginseng seedling. With the panax notoginseng seedling protection film provided by the invention, water loss of panax notoginseng seedling during a transplanting process can be reduced, and seedling surface skin damage caused by abrasion during transportation or during transplanting can be reduced. Therefore, fungal infection or insect attack caused by damages after transplanting can be reduced, occurrence rate of panax notoginseng root rot, sclerotinia stem rot, sheath blight, anthracnose, and blight can be reduced, and panax notoginseng survival rate can be improved. The panax notoginseng seedling protection film provided by the invention has good physiochemical performances such as film-forming performance, water resistance, air permeability, water permeability, and the like. Therefore, healthy growth of crops can be protected.

The invention belongs to the field of disease resistance detection of horticultural crops and relates to a seedling-stage root rot resistance identification method for vigna unguiculata. The method comprises the steps of carrying out germination hastening on vigna unguiculata seeds, carrying out sowing and seedling raising, carrying out special strain inoculating so as to carry out screening, preparing a vigna unguiculata root rot bacterium spore suspension, carrying out pathogenic bacterium infestation, carrying out onset examination and the like. Root systems of young seedlings are soaked and infested by adopting a bacterial solution so as to subject the vigna unguiculata young seedlings to artificial root rot bacterium infection; and the vigna unguiculata seeds are cultured in seedling raising matrixes until each young seedling has two leaves and one heart, and then, the young seedlings are drawn out for infestation, so that the growth vigor and size of the vigna unguiculata young seedlings are easily controlled, the vigna unguiculata young seedlings are easily drawn out, and the consistency and reliability of identification results are guaranteed. Special indicator strains are obtained through screening, the infesting effect is better by adopting appropriate time of infestation and appropriate root rot bacterium spore suspension concentration, the bacterium inoculating is uniform, and the onset is rapid. According to the method, by only injuring roots of different plants of consistent growth vigor through directly and naturally rubbing the roots and then infesting the roots with the indicator strains, the root rot resistance of a large amount of vigna unguiculata breeding materials can be identified simply, conveniently, rapidly and reliably, so that the efficiency of variety resistance identification is increased.

The invention relates to marine bacteria BA-3 (Bacillus amyloliquefaciens-3) and application thereof in orchid disease prevention and treatment, and belongs to the technical field of microorganisms. The preservation number for the marine bacteria BA-3 is CGMCC No.12745. The marine bacteria BA-3 capable of preventing and treating plant diseases, caused by stalk rot pathogens and root rot pathogens, of orchids can grow together with the orchids especially, can survive stably in orchid plants and can prevent and treat stalk rot diseases or root rot diseases of the orchids effectively.

The invention relates to heterocyclic substituted 1, 3, 4-oxadiazole hydrazide compounds and a preparation method and application thereof. The compound has a structure shown as a general formula (I). The compound has a relatively good inhibition effect on pathogenic fungi and bacteria; and the compound has good inhibition effects onfusarium graminearum, potato late blight bacteria, blueberry root rot bacteria, pepper fusarium wilt, sclerotinia sclerotiorum, colletotrichum gloeosporioides, botryosphaeria dothidea, rhizoctonia solani, xanthomonas oryzae pv., ralstonia solannacearum, Xanthomonas citri, kiwi canker bacteria, Xanthomonas oryzae cucumber, Xanthomonas oryza konjak, Botryosphaeria dothidea, tomato canker pathogens, apple canker pathogens and the like.

The invention belongs to the technical field of biological fermentation engineering, and specifically relates to a high-yield paenibacillus polymyxa strain and an application thereof. Paenibacillus polymyxa YSD7 is preserved at China General Microbiological Culture Collection Center on December 5, 2019, and a preservation number is CGMCC 19087. The paenibacillus polymyxa YSD7 can prevent and treaterwinia carotovora, taro soft rot bacteria, phytophthora nicotianae, flower root rot bacteria, bipolaris sorokiniana, cercospora personata, or polygonatum root rot bacteria. The inventors finally obtain the paenibacillus polymyxa strain YSD7 with relatively good growth vigor through the combination application of ultraviolet mutagenesis and diethyl sulfate mutagenesis technology, and qualitativescreening breeding based on the existing strain. Preliminary experimental results show that the highest fermentation activity of the YSD7 strain can reach 9.5 billion/mL, the YSD7 strain shows relatively good growth effects, and a good technical foundation for the preparation of paenibacillus polymyxa microbial preparation products can be laid.

The invention discloses a breeding method for rapidly screening resistant varieties of wheat root rot by using root rot fungus crude toxin. The method comprises the following steps of (1) selecting to-be-improved wheat variety/strain and wheat root rot resistance source and hybridizing to obtain F1; (2) backcrossing the F1 to obtain BCF1; (3) sowing male and female parents and the BCF1 at different stages; (4) treating the male and female parents by using root rot fungus crude toxin of gradient concentrations and selecting resistance screening pressure of the root rot; (5) treating the BCF1 by using the resistance screening pressure of the root rot to obtain BCF2; and (6) taking BCF2 for anther culture to obtain an anther culture family and screening according to root rot resistance and agronomic characters to obtain a new wheat strain with root rot resistance. Hybrid species are treated by selecting the root rot fungus crude toxin with a proper concentration at the hypersensitive stage of the root rot fungus crude toxin and the first division stage of zygote, thereby specifically screening the resistance wheat family for the root rot and greatly improving the work efficiency and the accuracy of resistant breeding of the root rot.

The invention provides Burkholderiaspp.B36. The strain has relatively strong inhibitory activity on main root rot bacteria of panax notoginseng, namely rust rot bacteria, has relatively obvious degradation on main saponin autotoxins (R1, Rg1, Re, Rb1 and Rd) of panax notoginseng, and especially has significant degradation activity on saponins Rb1 and Rd. The Burkholderiaspp.B36 has a broad application prospect.

The invention relates to application of 4'-demethyl epipodophyllotoxin as an agricultural bactericide. The compound has a relatively good inhibition effect on pathogenic bacteria and fungi; the invention aims at rice bacterial leaf blight, tobacco bacterial wilt, cucumber bacterial leaf blight, konjac bacterial leaf blight, citrus canker pathogen, grape canker pathogen, tomato canker pathogen, kiwi canker pathogen, apple canker pathogen, cucumber botrytis cinerea, pepper fusarium wilt pathogen, sclerotinia sclerotiorum, wheat gibberellic disease, potato late blight pathogen, and blueberry root rot bacteria, and the compound has a good inhibition effect.

The invention relates to 1, 3, 4-oxadiazole compounds containing a morpholine group as well as a preparation method and application thereof. The compound has a structure as shown in a general formula (I) which is described in the specification. On the basis of a derivative containing a 1, 3, 4-oxadiazole group, a morpholine group capable of improving the biological activity of a target compound is introduced into the system. The compound has a good inhibition effect on pathogenic fungi and bacteria, such as Xanthomonas oryzae pv. Oryzae, Xanthomonas axonopodis pv. citri, fusarium oxysporum, and blueberry root rot.

The invention relates to a seedling-stage identification method for zucchini root rot resistance, and belongs to the technical field of crop disease resistance identification. The method comprises thefollowing steps of: firstly, preparing a root rot fungus spore suspension by taking zucchini root rot fungus, wherein the concentration is 10<6>/mL; after zucchini seedling cotyledons are flattened,injecting the zucchini root rot fungus spore suspension at the root for inoculation; and after 20 days, investigating the disease stages of each plant, calculating the disease index, and evaluating the disease resistance of zucchini rootstock germplasm resources against the root rot fungus. The invention establishes a seedling-stage identification method for zucchini rootstock root rot resistance.The identification method is seedling-stage identification, is simple to operate, fast and efficient, has accurate and reliable identification result, can obviously distinguish the difference of theresistance of different zucchini rootstock materials to root rot, is suitable for identifying and screening root rot disease resistance of mass materials, and improves the screening efficiency of germplasm resource materials.

The invention belongs to the technical field of root rot, and discloses a prevention and treatment method for root rot of idesia polycarpa maxim seedlings transplanted in a field. Through deep furrowsand high ridges, the field humidity is reduced, and the field environment generated by the root rot is prevented; lime sulphur is used for soaking roots before idesia polycarpa maxim is transplanted,funguses are permeated and eroded, and root rot funguses are eliminated before then idesia polycarpa maxim seedlings are transplanted in the field; bacillus subtilis protects roots to protect the idesia polycarpa maxim from being damaged by pathogenic bacteria and kill the root rot funguses, so that the whole growth period of the idesia polycarpa maxim is no longer subject to the damage of funguses. The bacillus subtilis grows and propagates through nutrition competition, thereby preventing the growth of plant pathogenic bacteria through a mode of occupying living space, a layer of high-density protective film can be formed on the plant surface, so that the pathogenic bacteria cannot obtain the living space, and crops are protected from being damaged by the pathogenic bacteria. The bacillus subtilis can secretes antifungal substances, inhibit the germination of pathogen spores and the growth of hyphae, thereby achieving the purpose of prevention and treatment; the prevention and treatment rate of the root rot reaches 98%.

The invention relates to a thiazole hydrazide compound as well as a preparation method and an application thereof. The compound has a structure as shown in a general formula (I). The compound has a relatively good inhibition effect on pathogenic fungi and bacteria; and has a good inhibition effect onfusarium graminearum, potato late blight bacteria, blueberry root rot bacteria, pepper fusarium oxysporum, sclerotinia sclerotiorum, colletotrichum oryzae, botryosphaeria dothidea, rhizoctonia solani, xanthomonas oryzae pv. Oryzae, ralstonia solanacearum, citrus canker germs, kiwi fruit canker germs, cucumber xanthomonas oryzae pv. Cucumerinum, Xanthomonas oryzae of konjac, Botryosphaeria dothidea, tomato canker pathogen, apple canker pathogen and the like.

Relating to the technical field of pesticide compounding, the invention discloses a bactericidal composition and application thereof. The effective component of the bactericidal composition contains phenazine-1-carboxylic acid A and a carbamate bactericide B, wherein the carbamate bactericide B is one or more of propamocarb and its hydrochloride or diethofencarb and its hydrochloride, and the weight ratio of A to B is 1:100-100:1. Compounding of the effective component A and B has an obvious synergistic effect, and can effectively reduce the dosage of each single agent. The bactericidal composition has good effect on expansion of the bacteriocidal spectrum and delaying of plant resistance to drugs, also can prolong the lasting period, and has obvious promotion value. The composition can be added with other pesticide adjuvants to be processed into any dosage form. The bactericidal composition can effectively control sheath blight, rice blast, Ustilaginoidea virens, rust disease, root rot, sclerotinia sclerotiorum, smut, and blight, etc.

The invention relates to the technical field of molecular detection, and discloses a primer group and a kit containing the primer group. The primer group comprises an upstream primer F1 and a downstream primer R1, and the sequences of the upstream primer F1 and the downstream primer R1 are as follows: the upstream primer F1 is 5'-CGTGTCGCAGAAGAGGG-3'; the downstream primer R1 is 5'-AATAGGAAGCCGCTGAGC-3', and the invention also discloses an application of the primer group or the kit in rapid identification of pepper root rot bacteria. The application comprises the following steps: S1, extracting sample DNA; S2, taking the sample DNA as a template, and carrying out PCR amplification reaction by utilizing the primer group to obtain a PCR amplification product; and S3, analyzing a PCR amplification product. The primer group provided by the invention can specifically identify fusarium solani, and achieves the characteristics of high amplification efficiency, strong specificity and high accuracy; meanwhile, the rapid identification method established in combination with the primer group is quite simple, and fusarium solani can be identified without morphological identification and DNA sequencing.

The invention relates to a primer, a probe, a kit and detection method for detecting cotton root rot bacteria. the sequence of an upstream primer myaF is 5 '-GAGGGCATGCCTGTTCGA-3', the sequence of a downstream primer myaR is 5 '-CCAAGCCAACCCTTTTCAC-3', and the sequence of a probe myaT is 5 '-CGTCAGCATAACAACCTCAAGCACC-3'. A real-time fluorescence PCR detection method is adopted to detect the cotton root rot bacteria, compared with a traditional detection method, the method has the advantages of being short in detection period, high in specificity and sensitivity, free of PCR post-treatment and the like, and the requirements for rapid and accurate port detection are met. In addition, the primer and the probe have the advantages of being high in amplification efficiency, good in specificity, high in sensitivity and the like.