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Trichoderma koningiopsis T5-1 bacterial strain and application thereof in improvement of growth of panax notoginseng and prevention and control of root rot

A technology of Trichoderma pseudokonii and Trichoderma pseudokonii, applied in the field of plant disease prevention and control, can solve the problems of harsh ecological environment, long growth cycle, and narrow ecological width, so as to promote the growth of Panax notoginseng and inhibit root rot , the effect of mycelium development

Active Publication Date: 2017-04-19
CHINA AGRI UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Panax notoginseng has strict requirements on the ecological environment, narrow ecological range, and long growth cycle. With the increase of planting years, pathogenic bacteria in the soil continue to accumulate, and the incidence of diseases continues to increase, causing annual losses of 10%-40%. Seriously, it reaches more than 70%, which seriously affects the output and quality of Panax notoginseng, and becomes the main obstacle restricting the healthy development of the Panax notoginseng industry
The resulting large-scale single-area planting and the abuse of chemical pesticides have made the problem of Panax notoginseng pesticide residues more prominent

Method used

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  • Trichoderma koningiopsis T5-1 bacterial strain and application thereof in improvement of growth of panax notoginseng and prevention and control of root rot
  • Trichoderma koningiopsis T5-1 bacterial strain and application thereof in improvement of growth of panax notoginseng and prevention and control of root rot
  • Trichoderma koningiopsis T5-1 bacterial strain and application thereof in improvement of growth of panax notoginseng and prevention and control of root rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: Isolation and identification of Trichoderma pseudoconii T5-1 bacterial strain

[0038] 1. Isolation and purification of strains

[0039] Three-year-old Panax notoginseng tissue sample was obtained from Wenshan Prefecture, Yunnan Province, and the soil on the surface of the Panax notoginseng tissue was washed with tap water; the tissue block at the junction of the diseased and healthy tissues of the Panax notoginseng tissue was cut, and the size was about 4mm 2 Disinfect the cut tissue block in 1% sodium hypochlorite solution for 3 minutes, rinse it with sterile water for 3 times, and dry it fully on the sterilized filter paper sheet; place the tissue block on PDA added with streptomycin sulfate (100ppm) On the plate, culture it upside down in a constant temperature incubator at 28°C for 1-3 days, and observe it regularly; after the suspected colonies grow in the culture dish, immediately pick them up and transfer them to a fresh PDA plate for numbering and...

Embodiment 2

[0046] Example 2: The growth-promoting effect of Trichoderma pseudoconii T5-1 strain conidia suspension on Panax notoginseng

[0047] Inoculate Trichoderma pseudoconii T5-1 strain on PDA medium and culture in the dark at 28°C for 6 days to obtain colonies of Trichoderma pseudoconii T5-1 strain; add 20mL sterile water to the petri dish, scrape and wash the meristems on the surface The spores were filtered with 4 layers of sterilized gauze to obtain the conidia suspension of Trichoderma pseudoconii T5-1 with a concentration of 1×10 7 conidia / mL.

[0048] The annual Panax notoginseng plants and the biennial Panax notoginseng plants in the field were provided by the Panax notoginseng Science and Technology Park in Miao Township, Yunnan Province. The experimental design of randomized blocks was adopted, and the plot area / number of Panax notoginseng plants was set as follows: annual Panax notoginseng 0.5m×0.5m / about 100 plants; biennial Panax notoginseng 1.5m×0.5m / about 30 plants; ...

Embodiment 3

[0056] Example 3: Inhibitory effect of the filtrate of Trichoderma pseudoconii T5-1 strain on Phytophthora spp.

[0057] Inoculate Trichoderma pseudoconii T5-1 strain on PDA medium and culture in the dark at 28°C for 2 days to obtain colonies of Trichoderma pseudoconii T5-1 strain; take 20 pieces of 5mm mycelium and inoculate to 300mL PDB culture In a 500mL Erlenmeyer flask with 25°C and 180rpm / min, shake culture for 30 days, then filter with a vacuum pump to obtain the filtrate of Trichoderma pseudoconii T5-1 strain; Mix thoroughly according to the volume ratio of 1:4, pour the plate, about 20mL per plate; use a 5mm hole puncher, take the mycelium block of the edge of the Phytophthora perishi colony that has been activated for 4 days, place it in the center of a 9cm plate, and culture it for 10 days. The colony diameter of Phytophthora viridans was measured by the method, and the inhibition rate was calculated. The result is as figure 2 and image 3 , the filtrate of Tric...

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Abstract

The invention belongs to the technical field of prevention and control of plants diseases and particularly relates to a trichoderma koningiopsis T5-1 bacterial strain and an application thereof in improvement of growth of panax notoginseng and prevention and control of root rot. The bacterial strain is named trichoderma koningiopsis, and is assigned the accession number of CGMCC No. 12779 in China General Microbiological Culture Collection Center. The trichoderma koningiopsis T5-1 bacterial strain has developed mycelia and fluffy and dense aerial mycelia, and is free of generation of pigments. The trichoderma koningiopsis T5-1 bacterial strain can improve growth of panax notoginseng and also can inhibit growth of root rot fungus. The bacterial strain can be hyper-parasitized on the root rot fungus, so that spore production of the root rot fungus is inhibited by means of volatile organic compounds from the bacterial strain in a sealed condition. The bacterial strain lays a good foundation for development and utilization of bio-bacterial fertilizers.

Description

technical field [0001] The invention belongs to the technical field of plant disease prevention and control, and in particular relates to Trichoderma pseudoconii T5-1 strain and its application in promoting the growth of Panax notoginseng and preventing and controlling root rot. Background technique [0002] The most rapid and effective way to prevent and control plant diseases is to use chemical fungicides. However, the long-term and extensive use of chemical fungicides will cause environmental pollution, ecosystem damage, and the emergence of drug resistance and drug resistance of pathogenic bacteria. In addition, the problem of pesticide residues also seriously restricts the safe production of crops and threatens human health. At present, chemical pesticides are still dominant, but because bio-control bacteria have the advantages of being safe to use and not producing drug resistance, as a green and environmentally friendly means of prevention and control, especially addi...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P21/00C12R1/885
CPCA01N63/30C12N1/145C12R2001/885
Inventor 李健强李迎宾罗来鑫蒋娜宋聪聪曹永松刘屹湘朱书生杨敏姜治国
Owner CHINA AGRI UNIV
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