System and method for constructing a three-dimensional multi-scale vascularized stent

A construction method and vascularization technology, applied in the field of biomanufacturing, can solve the problems that cannot be effectively solved, have no branches, and the size of the vascular network is single, and achieve the effect of easy removal, easy material, and simple preparation process

Active Publication Date: 2019-01-04
SHANGHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although these two methods can produce a vascular network structure similar to blood vessels in human tissue, the vascular network produced in the first method is single in size and has no branches.
In the second method, the three-dimensional structure of the vascular network produced is two-dimensional accumulation or large in size, which is not suitable for the small-scale three-dimensional vascular network of the human body. These methods cannot effectively solve the problem of three-dimensional multi-scale vascularized network manufacturing in tissue scaffolds. The problem

Method used

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  • System and method for constructing a three-dimensional multi-scale vascularized stent
  • System and method for constructing a three-dimensional multi-scale vascularized stent
  • System and method for constructing a three-dimensional multi-scale vascularized stent

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Effect test

Embodiment example 1

[0029] Such as figure 1 As shown, the construction system of the three-dimensional multi-scale vascularized stent includes a 3D printer, a medical syringe, an air compressor and a high-voltage power supply. The three medical syringes are installed on the 3D printer, and the first and second medical syringes are connected with air compressors The machine phase is connected as the first and second nozzles of 3D printing for printing modified gelatin with Pluronic F127. The needle of the third medical syringe is connected to the positive pole of the high-voltage power supply as the third nozzle of 3D printing, and the receiving platform of the 3D printer is connected to the ground wire of the high-voltage power supply for electrohydrodynamic direct writing. Including the following steps:

[0030] a. Print the hemispherical bracket (7) used to receive the sacrificial template on the 3D printer receiving platform (5): configure 15 ± 0.5% hydrogel with deionized water, place it in ...

Embodiment 2

[0035] see Figure 1 to Figure 5 , the construction method of the three-dimensional multi-scale vascularized stent, comprising the following steps:

[0036] a. Print the hemispherical bracket (7) used to receive the sacrificial template on the 3D printer receiving platform (5): configure 15±0.5% gelatin with deionized water, place it in a 60±2°C water bath and stir until the gelatin particles Dissolve evenly and the temperature drops to 30±1°C. At this time, add mTG enzyme (the mass ratio of gelatin to mTG enzyme is 10:1) to cross-link the gelatin solution, heat the prepared gelatin solution to a molten state and keep it in the first In a medical syringe, use the first nozzle of the bioprinter to print out the hemispherical bracket (7) from the molten gelatin (6) on the receiving platform (5) of the 3D printer;

[0037] b. Print the main channel and secondary channel sacrificial template of the vascular network (9): use deionized water to configure 35±1% Pluronic F127, stir a...

Embodiment example 3

[0041] This implementation case is basically the same as the implementation case 2, and the special features are as follows:

[0042] see Figure 1 ~ Figure 3 , taking advantage of the printability of Pluronic F127 and the electrospinnability of PCL and both are easy to remove, the Pluronic F127 and PCL solutions were printed on the printed hydrogel hemispheres by 3D printing and electrohydrodynamic direct-writing printing, respectively. The designed sacrificial model is printed on the printed circuit board, and then the gelatin-wrapped sacrificial template with a configured concentration is printed to obtain a scaffold containing three-dimensional multi-scale vascularized channels. First, the configured hydrogel is heated to a molten state and kept loaded into the first medical syringe, and the molten gelatin is printed out of a hemispherical bracket on the receiving platform of the 3D printer using the first nozzle of the bioprinter, and then a hemispherical scaffold is prin...

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Abstract

The invention discloses a system and method for constructing a three-dimensional multi-scale vascularized stent. The system comprises a 3D printer, a medical syringe, an air compressor and a high-voltage electric power. The method adopts a biological 3D printing technology combined with electrohydrodynamics direct writing to manufacture a stent containing a three-dimensional multi-scale vascularized network channel by a composite method of increasing and decreasing material manufacturing technology. As that three-dimensional shape of the sacrificial template require by the invention is imparted by the shape of the prin gelatin hemisphere, no harmful substances are generated in the whole process and the material is easy to obtain. By means of the printability of gelatin, Plannick F127 and PCL, three-dimensional and multi-scale vascularized stents can be fabricated, which solves the problem that three-dimensional and multi-scale vascularized stent network can not be obtained by biological 3D printing alone. It is of great significance to solve the vascularization problem of human tissue repair in clinical medicine.

Description

technical field [0001] The invention relates to a construction system and method for a three-dimensional multi-scale vascularized stent, which is applied in the technical field of biomanufacturing. Background technique [0002] Over the past few years, research related to tissue engineering, such as the fabrication of nerves, skin, ears, and cartilage, has grown considerably. However, complex organ and in vitro tissue fabrication remains a great challenge in this field. One of the most important issues in this fabrication process is the formation of the three-dimensional vascular network. In the engineered tissue structure, cells must be close enough to oxygen and nutrient supply (100-200 μm) to prevent their necrosis, while the uniformly distributed three-dimensional blood vessel and capillary network also provide channels for the delivery of metabolites. Therefore, the formation of vascularization in engineered tissue scaffolds is important for tissue regeneration and fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/06B33Y10/00
CPCA61F2/06A61F2240/001B33Y10/00
Inventor 胡庆夕沈显虎张海光
Owner SHANGHAI UNIV
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