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A method for alleviating the autotoxic injury of Panax notoginseng by using oxalic acid

A technology of notoginseng and oxalic acid, which is applied in the direction of botany equipment and methods, application, animal husbandry, etc., can solve the problems of continuous cropping obstacles restricting the production of notoginseng, no public literature reports, etc., to increase the probability of root rot damage, Effect of reducing the use of pesticides and improving quality

Active Publication Date: 2020-06-16
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Panax notoginseng is one of the most valuable Chinese herbal medicines in my country, but continuous cropping obstacles seriously restrict the production of panax notoginseng
After searching, there is no public literature report identical with the present invention

Method used

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  • A method for alleviating the autotoxic injury of Panax notoginseng by using oxalic acid
  • A method for alleviating the autotoxic injury of Panax notoginseng by using oxalic acid
  • A method for alleviating the autotoxic injury of Panax notoginseng by using oxalic acid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Embodiment 1: add oxalic acid with exogenous source and process Radix Notoginseng root system and alleviate autotoxic saponin Rg 1 damage to the root system

[0008] After the annual Panax notoginseng seedlings grow in the experimental shade for 7 months, select healthy Panax notoginseng plants and bring them back to the laboratory, rinse the soil on the roots with tap water, rinse them with sterile water three times, and put them into a 200mL tissue culture bottle Perform liquid culture. In order to exclude the influence of 1‰ methanol on the root system of Panax notoginseng, 1‰ methanol was added to all treatments. A total of 5 treatments were set up in the test, that is, the concentration of 1.0mg / L Rg 1 Treat 0h, 3h, 12h, 24h and 48h respectively. Four repetitions were set up for each treatment, and eight Panax notoginseng seedlings were placed in each repetition. The test adopts the reverse sequence method for treatment. All treatments are sampled after hydropo...

Embodiment 2

[0016] Embodiment 2: Utilize oxalic acid root irrigation to alleviate the autotoxic injury of autotoxic saponin crude extract to Panax notoginseng

[0017] A randomized block experimental design was used. Sow Panax notoginseng into the new soil that has not been planted with Panax notoginseng, and the new soil with the concentration of 1.0mg / L and 5.0mg / L crude extract of autotoxic saponins. Within 7 days after the seeds of Panax notoginseng were sown, the roots were irrigated once with oxalic acid solutions with concentrations of 0, 0.1, 1.0, 5.0 and 10.0 mg / L prepared in deionized water containing 0.01% Tween 80. After the germination rate of Panax notoginseng seeds reaches 10%, the oxalic acid solution of the same concentration is continuously watered for 3 times, with an interval of 7 days between each time. The root irrigation volume of each Panax notoginseng is 5mL. Three repetitions were set up for each treatment, and 1000 Panax notoginseng seeds were sown in each rep...

Embodiment 3

[0021] Embodiment 3: oxalic acid root irrigation treatment relieves autotoxic saponin monomer Rg 1 The autotoxic effect on Panax notoginseng

[0022] A randomized block experimental design was used. Sow Panax notoginseng into new soil that has not been planted with Panax notoginseng, and add the autotoxic saponin monomer Rg at a concentration of 1.0mg / L (or 5.0mg / L) 1 in the new soil. Within 7 days after the seeds of Panax notoginseng were sown, the roots were irrigated once with oxalic acid solutions with concentrations of 0, 0.1, 1.0, 5.0 and 10.0 mg / L prepared in deionized water containing 0.01% Tween 80. After the germination rate of Panax notoginseng seeds reaches 10%, the oxalic acid solution of the same concentration is continuously watered for 3 times, with an interval of 7 days between each time. The root irrigation volume of each Panax notoginseng is 5ml. Three repetitions were set up for each treatment, and 1000 Panax notoginseng seeds were sown in each replicat...

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Abstract

The invention discloses a method for alleviating panax notoginseng self-poisoning injuries by using oxalic acid. The method particularly comprises the following steps: preparing an oxalic acid solution of which the concentration is 0.1 to 5.0mg / L by using deionized water containing 0.01% of Tween 80 in percent by volume, carrying out root-irrigation once after panax notoginseng is sowed or transplanted for 7 days; then continuously irrigating for 3 times by using the oxalic acid solution with the same concentration when the germination rate of panax notoginseng seeds reaches 10%, wherein irrigation is carried out at an interval of 7 days, and the root-irrigation amount for each panax notoginseng is 5mL. The method for alleviating the panax notoginseng self-poisoning injuries by using the oxalic acid disclosed by the invention has the beneficial effects of solving the problem of decreased oxygen free radical scavenging abilities for the reduced oxalic acid taken as a panax notoginseng rhizosphere antioxidant substance caused by saponin autotoxins, improving the emergence rate and growth potentials of the panax notoginseng seeds and seedlings, enhancing the panax notoginseng resistance, lowering the probability in increasing of root rot damage for cell damage caused by the autotoxins in the growth process of the panax notoginseng and reducing the use amount of pesticides, thereby being an ecological, safe and effective measure for alleviating continuous cropping obstacles. The method disclosed by the invention is of great significance to improve the quality of the panax notoginseng and lower pesticide residues.

Description

technical field [0001] The invention relates to a method for using oxalic acid to alleviate autotoxic damage of notoginseng, belonging to the technical field of plant protection. Background technique [0002] Panax notoginseng is one of the bulk valuable Chinese herbal medicines in my country, but continuous cropping obstacles seriously restrict the production of panax notoginseng. Existing studies have shown that the soil that has been planted with Panax notoginseng cannot be continuously cropped after 10 to 20 years, and the obstacle of continuous cropping has become the main factor restricting the healthy development of the Panax notoginseng industry. Over the years, many scholars have carried out in-depth research on the causes of continuous cropping obstacles of Panax notoginseng, and believe that continuous cropping obstacles are mainly related to the infection of root rot fungi, the accumulation of autotoxic substances, the deterioration of soil physical and chemical ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N25/32
CPCA01N25/32
Inventor 杨敏朱书生钏有聪许彦国刘屹湘黄惠川何霞红朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY
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