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Application of 4'-demethyl epipodophyllotoxin as agricultural bactericide

A technology for demethylating epipodophyllotoxins and toxins, which is applied in the field of pesticide applications and can solve problems such as reducing the targeting of compounds

Inactive Publication Date: 2021-11-05
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2012, Kim et al. further analyzed the structure-activity relationship of todopol, and the results showed that when the A ring was introduced into the indole structure, its antibacterial activity could be significantly enhanced, but the targeting of the compound to FtsZ was reduced.

Method used

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  • Application of 4'-demethyl epipodophyllotoxin as agricultural bactericide
  • Application of 4'-demethyl epipodophyllotoxin as agricultural bactericide
  • Application of 4'-demethyl epipodophyllotoxin as agricultural bactericide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The inhibitory rate of 4'-desmethylepipodophyllotoxin on plant pathogenic bacteria was tested by turbidimetric method, and the test objects were bacterial blight of rice (Xoo), bacterial wilt of tobacco and canker sore of citrus (Xac). DMSO was dissolved in the medium as a blank control. Put the rice bacterial blight bacteria (the rice bacterial blight pathogen in the M210 solid medium) into the NB medium, shake and cultivate it in a constant temperature shaker at 28°C and 180rpm until the logarithmic growth phase is ready for use; put the tobacco bacterial wilt bacteria (in the M210 solid medium) M210 solid medium) was placed in NB medium, shaken and cultivated in a constant temperature shaker at 28°C and 180rpm until the logarithmic growth phase was ready for use; put X. citri (on M210 solid medium) into NB medium, Shake the culture in a constant temperature shaker at 28°C and 180 rpm until the logarithmic growth phase is ready for use. Add 5 mL of poisonous NB liqui...

Embodiment 2

[0036] Before the rice plants are used, the dead and old leaves are first subtracted, and the sample number is marked on the side of the pot with an oily marker before treatment, and the rice is neatly placed on the test bench; the spraying process is carried out with a watering can with a good atomization effect. It is advisable to require that the leaves of the plants can shed water droplets; try to ensure that two pots of healthy plants are tested for each drug; the cutting position is 0.5-1.0 cm away from the top of the rice, and 15-20 leaves are inoculated in each pot; the newly activated rice on the plate Add appropriate amount of Xanthomonas to NA medium, and cultivate overnight at 28°C and 180rpm until the turbidity of the medium is OD 595 =0.6 can be used for future use.

[0037] 1) Therapeutic activity:

[0038] First, at about 0.5-1.0cm of the leaves of healthy plants, use the scissors dipped in bacterial liquid to cut off the upper leaves at a slight angle, and di...

Embodiment 3

[0054] Before using the rice plants, cut off the dead and old leaves, mark the sample number on the side of the pot with an oil-based marker before treatment, and place the test materials neatly on the test bench; use a watering can with a good atomization effect for the spraying process , it is advisable to require that the leaves of the plants can shed water droplets; try to ensure that two pots of healthy plants are tested for each drug;

[0055] First, ensure that each liquid medicine corresponds to two pots of healthy plants, and spray about 25mL evenly on the front and back of the leaves of the two pots of rice until the water droplets evenly cover the surface of the leaves of the plants. The control group and the blank group were operated in the same way. After spraying, put the sprayed plants in an outdoor light-proof and ventilated place for about 1 hour. After the water droplets on the surface of the leaves are completely dry, put them in a light incubator with a tem...

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Abstract

The invention relates to application of 4'-demethyl epipodophyllotoxin as an agricultural bactericide. The compound has a relatively good inhibition effect on pathogenic bacteria and fungi; the invention aims at rice bacterial leaf blight, tobacco bacterial wilt, cucumber bacterial leaf blight, konjac bacterial leaf blight, citrus canker pathogen, grape canker pathogen, tomato canker pathogen, kiwi canker pathogen, apple canker pathogen, cucumber botrytis cinerea, pepper fusarium wilt pathogen, sclerotinia sclerotiorum, wheat gibberellic disease, potato late blight pathogen, and blueberry root rot bacteria, and the compound has a good inhibition effect.

Description

technical field [0001] The present invention relates to the technical field of pesticide application, relates to the use of 4'-desmethyl epipodophyllotoxin, specifically relates to the use of 4'-desmethyl epipodophyllotoxin as an agricultural fungicide. Background technique [0002] In recent years, plant bacteria and fungi have seriously affected the yield and quality of crops worldwide, causing huge economic losses to farmers. For example, rice bacterial blight (Xanthomonas oryzaepv.Oryzae) is a rod-shaped Gram-negative bacterium that can cause the leaves of rice to wither and turn white, and it brings at least 10-50% of rice-growing countries every year. Production reduction, serious or even no harvest. Additionally, Xanthomonas axonopodis pv. Citri causes citrus rot, affecting citrus yields globally. In the process of agricultural production, due to the long-term use of traditional medicaments, plant pathogenic bacteria have developed certain resistance to them. There...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01P3/00A01P1/00A01G13/00
CPCA01N43/90A01G13/00
Inventor 王培义周翔李虎叶豪杰邵武斌柳立伟
Owner GUIZHOU UNIV
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