Compound enzyme for pretreatment of heavy metals detection in food with high fat content

A fat content and heavy metal technology, applied in the preparation of test samples, enzymes, hydrolase, etc., can solve the problems of complex pretreatment of heavy metals, difficult on-site detection, poor environmental protection, etc., and achieves no need for corrosive reagents and short pretreatment time. , mild effect

Pending Publication Date: 2021-01-08
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a kind of compound enzyme and the pretreatment method that are used for the detection pretreatment of heavy metals in foods with high fat content such as peanuts and soybeans. Starch, fat and other components that are easy to combine with heavy metals, and release cadmium and lead ions, solve the long-standing problem of harmful heavy metals in soybeans and peanuts and their products. Detection and other issues

Method used

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  • Compound enzyme for pretreatment of heavy metals detection in food with high fat content
  • Compound enzyme for pretreatment of heavy metals detection in food with high fat content

Examples

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Effect test

Embodiment 1

[0020] Composite enzyme suitable for pretreatment of heavy metal detection in soybean and peanut samples. The specific preparation and application process are as follows:

[0021] 1) Add about 1g soybean or peanut sample, 0.57g compound enzyme (into a 10mL centrifuge tube, wherein the mass percentage of compound enzyme is composed of: 24.6% amylase, 60.4% protease, 7.5% lipase, 7.5% cellulase ;

[0022] 2) Add 10 mL of water into the centrifuge tube as an extraction solvent, and then extract at 55° C. for 6 minutes to obtain a mixture;

[0023] 3) The mixture was centrifuged at 12000 rpm for 3 minutes to separate the aqueous phase and the solid phase, after which the aqueous phase sample solution was analyzed. Before analysis, the sample solution was filtered through a 0.22 μm nylon membrane filter to remove insoluble particles in the sample to ensure the uniformity of the analysis and the accuracy of the analysis results. The spiked recovery data of the actual samples are s...

Embodiment 2

[0028] A compound enzyme used for pretreatment of heavy metal detection in foods with high fat content such as peanuts and soybeans, comprising the following components and contents in parts by weight: 22 parts of amylase, 62 parts of protease, 5 parts of lipase and 10 parts of cellulase .

[0029] The preparation method of the composite enzyme is as follows: uniformly mixing each component to obtain the composite enzyme.

[0030] In application, the complex enzyme is used for the pretreatment of soybean or peanut samples, hydrolyzes the components combined with heavy metals in the samples, and releases cadmium ions and lead ions in them for heavy metal detection.

Embodiment 3

[0032] A compound enzyme used for pretreatment of heavy metal detection in foods with high fat content such as peanuts and soybeans, comprising the following components and contents by weight: 26 parts of amylase, 58 parts of protease, 10 parts of lipase and 5 parts of cellulase .

[0033] The preparation method of the composite enzyme is as follows: uniformly mixing each component to obtain the composite enzyme.

[0034] In application, the complex enzyme is used for the pretreatment of soybean or peanut samples, hydrolyzes the components combined with heavy metals in the samples, and releases cadmium ions and lead ions in them for heavy metal detection.

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Abstract

The invention discloses to a compound enzyme for pretreatment of heavy metals detection in food with high fat content. The invention relates to a compound enzyme and a pretreatment method for detecting heavy metals in foods with high fat content, such as peanuts and soybeans. The compound enzyme comprises the following components in parts by weight: 22-26 parts of amylase, 58-62 parts of protease,5-10 parts of lipase and 5-10 parts of cellulase. The pretreatment method comprises the following steps: uniformly mixing a sample to be detected with the compound enzyme, adding an extraction solvent for extraction, and then centrifuging and filtering to obtain a water phase as the sample solution to be detected . Compared with the prior art, heavy metals in soybean and peanut samples can be extracted within about 6 minutes, the method provided by the invention has short pretreatment time, mild action conditions and high heavy metal recovery rate, and has the advantages of simple operation,no need of corrosive reagents, no need of high temperature and specific digestion instruments, etc; and solves the problems of complex pretreatment, time-consuming, poor environmental protection and difficult for on-site detection, which existing in soybeans, peanuts and their products for a long time.

Description

technical field [0001] The invention belongs to the technical field of food, and relates to a compound enzyme and a pretreatment method for detecting and pretreating heavy metals in foods with high fat content such as peanuts and soybeans. Background technique [0002] With the increasing mining, smelting, processing and commercial manufacturing activities of heavy metals, many heavy metals such as lead and cadmium enter food such as food through the atmosphere, water and soil, and cause serious food pollution. Different from the degradable characteristics of most pollutants, heavy metal pollutants have long half-lives and are difficult to metabolize. Therefore, after entering the human body through the food chain, they generally cause chronic damage to the body through an accumulation effect, which in turn causes deformities, cancer and other toxic effects. [0003] At present, mass spectrometry, Raman spectrometry and atomic absorption spectrometry are mostly used to detec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/26C12N9/50C12N9/20C12N9/42G01N1/28
CPCC12N9/2411C12N9/50C12N9/20C12N9/2437G01N1/28C12Y301/01003
Inventor 徐斐曹慧孟登辉袁敏叶泰于劲松黎燕吴秀秀阴凤琴
Owner UNIV OF SHANGHAI FOR SCI & TECH
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