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Method for quantitatively detecting content of fat-soluble vitamins in peripheral blood sample

A quantitative detection and vitamin technology, applied in the field of detection, can solve the problems of difficulty in developing a whole blood fat-soluble vitamin detection method, inability to achieve simultaneous detection of fat-soluble vitamins, inability to synchronize detection of fat-soluble vitamins, etc. Short time, reducing the effect of matrix effects

Pending Publication Date: 2021-07-06
杭州凯莱谱精准医疗检测技术有限公司
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Problems solved by technology

Compared with serum samples, in the detection of fat-soluble vitamins, components such as red blood cells in whole blood samples will cause serious matrix effects in the detection of fat-soluble vitamins, which has caused great difficulties in the development of detection methods for fat-soluble vitamins in whole blood
In the existing technologies for the detection of fat-soluble vitamins in peripheral blood that have been disclosed so far, each scheme can only detect one or two of the fat-soluble vitamins, and cannot simultaneously detect two or more fat-soluble vitamins
In the aforementioned prior art of detecting fat-soluble vitamins in peripheral blood and whole blood by using liquid chromatography tandem mass spectrometry, the sample pretreatment steps used are complex, including protein precipitation, liquid-liquid extraction, drying of the extract, and reconstitution of the analyte and other steps, the operation is complicated, time-consuming and labor-intensive, and it is difficult to realize high-throughput automatic operation, and it is impossible to realize the simultaneous detection of more than two fat-soluble vitamins

Method used

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  • Method for quantitatively detecting content of fat-soluble vitamins in peripheral blood sample
  • Method for quantitatively detecting content of fat-soluble vitamins in peripheral blood sample
  • Method for quantitatively detecting content of fat-soluble vitamins in peripheral blood sample

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Embodiment 1

[0043](1) Configuration of the standard curve: Dissolve the accurately weighed standard substances of vitamin A, vitamin D2, vitamin D3 and vitamin E in ethanol to obtain the corresponding standard stock solution, and then dilute the above stock solution with PBS to the following table 2 Standard curve showing concentration.

[0044] Table 2: Standard Curve Concentrations

[0045] Analyte Linear range (ng / mL) SD1 SD2 SD3 SD4 SD5 SD6 Vitamin D3 5～250 5 10 25 50 100 250 Vitamin D2 2～100 2 4 10 20 40 100 Vitamin A 40～2000 40 80 200 400 1000 2000 Vitamin E 500～25000 500 1000 2500 5000 10000 25000

[0046] (2) Configuration of protein sedimentation agent: dissolve accurately weighed vitamin A-d6, vitamin D3-d6 and vitamin E-d6 with ethanol to obtain the corresponding isotope internal standard stock solution, and then mix the above isotope internal standard stock solution with ethanol , diluted to a certain...

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Abstract

The invention relates to a method for quantitatively detecting a content of an antiviral drug in a plasma sample. The method comprises the following steps: (1) collecting trace peripheral blood; (2) mixing the peripheral blood whole blood sample with the protein precipitation solution containing an interior label, carrying out vortex and centrifugation, and diluting the supernatant to obtain a to-be-detected solution; and (3) detecting and analyzing the to-be-detected solution by adopting a high performance liquid chromatography-tandem mass spectrometry system. The invention provides the method for simultaneously detecting the vitamin A, the vitamin D2, the vitamin D3 and the vitamin E in the peripheral blood sample, the pretreatment is simple, the detection cost is low, and the detection efficiency is high.

Description

technical field [0001] The invention relates to the technical field of detection, and mainly relates to a method for quantitatively detecting the content of fat-soluble vitamins in peripheral blood samples. Background technique [0002] Fat-soluble vitamins refer to a class of vitamins that are easily soluble in organic solvents but not in water, mainly including vitamin A, vitamin D and vitamin E. These vitamins can be dissolved in fat, are not easy to be excreted, can be stored in the body, and play a very important and indispensable role in the metabolism and function of the human body. Vitamins A, D, and E participate in the synthesis, decomposition, and transformation of proteins, enzymes, hormones, and vitamins in the form of enzymes and prosthetic group activators, and affect physiological activities such as human growth and development, metabolism, and immune regulation. Therefore, it is very important to keep the level of fat-soluble vitamins in the body in an appr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/045
Inventor 刘鹏云刘妍妍马金飞刘华芬
Owner 杭州凯莱谱精准医疗检测技术有限公司
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