Method for quantitatively detecting content of fat-soluble vitamins in peripheral blood sample

Abstract

The invention relates to a method for quantitatively detecting a content of an antiviral drug in a plasma sample. The method comprises the following steps: (1) collecting trace peripheral blood; (2) mixing the peripheral blood whole blood sample with the protein precipitation solution containing an interior label, carrying out vortex and centrifugation, and diluting the supernatant to obtain a to-be-detected solution; and (3) detecting and analyzing the to-be-detected solution by adopting a high performance liquid chromatography-tandem mass spectrometry system. The invention provides the method for simultaneously detecting the vitamin A, the vitamin D2, the vitamin D3 and the vitamin E in the peripheral blood sample, the pretreatment is simple, the detection cost is low, and the detection efficiency is high.

Description

technical field [0001] The invention relates to the technical field of detection, and mainly relates to a method for quantitatively detecting the content of fat-soluble vitamins in peripheral blood samples. Background technique [0002] Fat-soluble vitamins refer to a class of vitamins that are easily soluble in organic solvents but not in water, mainly including vitamin A, vitamin D and vitamin E. These vitamins can be dissolved in fat, are not easy to be excreted, can be stored in the body, and play a very important and indispensable role in the metabolism and function of the human body. Vitamins A, D, and E participate in the synthesis, decomposition, and transformation of proteins, enzymes, hormones, and vitamins in the form of enzymes and prosthetic group activators, and affect physiological activities such as human growth and development, metabolism, and immune regulation. Therefore, it is very important to keep the level of fat-soluble vitamins in the body in an appr...

AI Technical Summary

Problems solved by technology

Compared with serum samples, in the detection of fat-soluble vitamins, components such as red blood cells in whole blood samples will cause serious matrix effects in the detection of fat-soluble vitamins, which has caused great difficulties in the development of detection methods for fat-soluble vitamins in whole blood

In the existing technologies for the detection of fat-soluble vitamins in peripheral blood that have been disclosed so far, each scheme can only detect one or two of the fat-soluble vitamins, and cannot simultaneously detect two or more fat-soluble vitamins

In the aforementioned prior art of detecting fat-soluble vitamins in peripheral blood and whole blood by using liquid chromatography tandem mass spectrometry, the sample pretreatment steps used are complex, including protein precipitation, liquid-liquid extraction, drying of the extract, and reconstitution of the analyte and other steps, the operation is complicated, time-consuming and labor-intensive, and it is difficult to realize high-throughput automatic operation, and it is impossible to realize the simultaneous detection of more than two fat-soluble vitamins

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