Use of HDAC2 in preparation of marker for evaluating progress of hepatic inflammation of acute hepatic failure

A technology of acute liver failure and markers, applied in the field of bioengineering, to achieve highly reliable results

Inactive Publication Date: 2021-01-08
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although HDAC3 has a nuclear input signal and a nuclear output signal, it is only found in the nucleus so far.

Method used

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  • Use of HDAC2 in preparation of marker for evaluating progress of hepatic inflammation of acute hepatic failure
  • Use of HDAC2 in preparation of marker for evaluating progress of hepatic inflammation of acute hepatic failure
  • Use of HDAC2 in preparation of marker for evaluating progress of hepatic inflammation of acute hepatic failure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Serum HDAC2 activity, serum CRP level, serum neutrophil percentage (Neu%), serum IL-1β level, serum IL-18 Level. All research subjects took 5ml of fasting cubital venous blood and placed it in a vacuum blood collection tube, and placed the blood sample in an automatic biochemical analyzer to detect the content of CRP and Neu%. The tested samples were centrifuged at 3000r / min for 3min to separate the serum. ELISA kits were used to detect serum IL-1β, IL-18, and HDAC2 levels. In the process of adding samples, blank wells, standard wells, and sample wells to be tested were respectively set. Add 100 μl of sample diluent to the blank well, and add 100 μl of standard or test sample to the remaining wells. Cover the microtiter plate and incubate at 37°C for 90 minutes. Discard the liquid, shake it dry, add 100 μl of Detection Ab working solution (prepared within 15 minutes before use) to each well, add a membrane to the microtiter plate, and incubate at 37°C for 1 hour. D...

Embodiment 2

[0026]The expression of HDAC2 in liver tissue was detected by immunohistochemical method. The cut tissues (<5mm3) were washed with normal saline, and immediately fixed in 4% paraformaldehyde (PFA) for 24 hours. After the material is fixed, it is washed with water for several hours to remove the fixation solution and crystal precipitation in the tissue. The tissues were dehydrated in 70%, 80%, and 90% ethanol solutions at various levels in turn, for 30 minutes each, and then put in 95%, 100% ethanol solutions for 2 times, each time for 20 minutes. Wet the material with a mixture of pure alcohol and xylene for 1-2 hours, and then replace it with a pure transparent agent (xylene, benzene, chloroform, n-butanol). Then put it into a mixture of xylene and paraffin, and then soak it in melted paraffin. After dipping in wax, put it into the embedding box and use paraffin for embedding. The wax block embedding the small intestine sample was cut into 3 mm slices. Endogenous peroxida...

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Abstract

The invention provides a marker for evaluating progress of a hepatic inflammation of an acute hepatic failure sufferer. The marker comprises histone deacetylase 2. The invention further provides use of the histone deacetylase 2 in preparation of the marker for evaluating the progress of the hepatic inflammation of the acute hepatic failure sufferer. The inflammation degree of hepatic tissue of theacute hepatic failure sufferer is evaluated through assaying amplitude of activity of the histone deacetylase 2, the reliability is high, and thus, a novel theoretical basis is provided for clinically evaluating the progress of the hepatic inflammation of the acute hepatic failure sufferer.

Description

technical field [0001] The patent of the invention belongs to the field of bioengineering and relates to histone deacetylase 2, specifically the use of histone deacetylase 2 in the preparation of markers for evaluating the progress of liver inflammation in patients with acute liver failure. Background technique [0002] Acute liver failure (ALF) is a clinically critical and severe disease in which multiple factors cause severe dysfunction of liver synthesis, detoxification and metabolism. Its pathology is characterized by massive necrosis of liver cells, accompanied by severe degeneration of surviving liver cells. ALF affects all organ systems, is resource intensive, and has a 30% mortality rate. At present, the medical treatment for ALF is limited to etiological treatment and symptomatic treatment, but there is still no effective treatment for its pathogenesis. At present, there is a lack of effective indicators for the early diagnosis of ALF. When ALF is diagnosed, the l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/118
Inventor 龚作炯王瑶王鲁文李汛焦方舟陈倩石春霞
Owner WUHAN UNIV
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