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Ultrathin I/II type collagen composite scaffold and preparation method thereof

A composite scaffold and collagen scaffold technology, applied in the field of biomedicine, can solve the problems of destroying the balance of type I and type III collagen, destroying the strength and integrity of fascia tissue, etc., and achieve the effect of preventing or treating arthritis and low antigenicity

Inactive Publication Date: 2021-01-12
CHANGZHOU NO 2 PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Smoking causes an increase in type III collagen, which disrupts the balance of type I and type III collagen, thereby destroying the strength and integrity of fascial tissue, which in turn leads to the occurrence of hernias
Therefore, type I / III double-layer collagen scaffolds are not the most suitable double-layer collagen scaffolds for the human body

Method used

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  • Ultrathin I/II type collagen composite scaffold and preparation method thereof
  • Ultrathin I/II type collagen composite scaffold and preparation method thereof
  • Ultrathin I/II type collagen composite scaffold and preparation method thereof

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Embodiment Construction

[0042]A preparation method of ultra-thin type I / II collagen composite scaffold, which is characterized in that it comprises the following steps:

[0043](1) Extract type I collagen;

[0044](2) Extract type II collagen;

[0045](3) Manufacturing molds;

[0046](4) Preparation of type I / II collagen composite scaffold.

[0047]The preparation method of type I collagen is as follows:

[0048](1) Take fresh pig Achilles tendon, wash it with ultrapure water several times, and continue washing several times after trimming impurities;

[0049](2) Cut the pig Achilles tendon tissue into small pieces, soak it in 75% alcohol for 10 minutes, wash it with ultrapure water several times, place it in 0.05mol / L acetic acid solution, take it out and stir it with a magnetic stirrer at 4℃ 24 hours;

[0050](3) Stir the pieces of pork Achilles tendon treated in step (2) with a pulverizer to a latex-like liquid and collect it in a 1 liter beaker;

[0051](4) Add 1g / L pepsin to the beaker and mix it with a magnetic stirrer at 4℃ f...

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Abstract

The invention belongs to the technical field of biomedicine, and particularly relates to an ultrathin I / II type collagen composite scaffold for cartilage repair in tissue engineering and a preparationmethod of the ultrathin I / II type collagen composite scaffold. Compared with type III collagen, the type II collagen adopted by the invention is more consistent with the composition of articular cartilage of a human body, accounts for 90-95% of extracellular matrix components of the articular cartilage, and plays a role in preventing or treating arthritis. II-type collagen is a macromolecular protein, filamentous collagen fibers of the II-type collagen are interwoven with elastin and proteoglycan protein to form a net-shaped structure, and certain mechanical strength is generated, which is particularly important for improving the strength of the scaffold. The I / II type collagen composite scaffold has extremely low antigenicity, can be completely degraded in a human body, and meets the requirements of excellent tissue engineering scaffold materials.

Description

Technical field[0001]The invention belongs to the field of biomedicine technology, and specifically relates to an ultra-thin type I / II collagen composite scaffold for cartilage repair in tissue engineering and a preparation method thereof.Background technique[0002]Scientists from Vefigen, Germany, first chose the type I / III double-layer collagen scaffold as the sealing material for autologous soft cell transplantation, which brought rapid development to the development of autologous chondrocyte transplantation technology and biological scaffold. Type III collagen is the main collagen in human skin, fascia, and tendons, and the ratio of type I collagen is 4 to 1. Type III collagen is immature, unstable, and has low elastic tension. Smoking will increase the type III collagen, destroy the balance between type I and type III collagen, thereby destroying the strength and integrity of the fascia tissue, and leading to the occurrence of hernia. Therefore, the type I / III double-layer colla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/24A61L27/50A61L27/58
CPCA61L27/24A61L27/50A61L27/58A61L2300/252A61L2300/41A61L2430/06
Inventor 韩龙
Owner CHANGZHOU NO 2 PEOPLES HOSPITAL
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