Biochemical oxygen demand microfluidic detection equipment and biochemical oxygen demand microfluidic detection method based on bacterial microcapsules

Abstract

The invention discloses biochemical oxygen demand microfluidic detection equipment and a biochemical oxygen demand microfluidic detection method based on bacterial microcapsules. The detection equipment comprises a multi-channel micro-fluidic chip, a porous optical fiber probe, a fluorescence detector and a light source, the multi-channel micro-fluidic chip comprises a sample injection channel, astorage channel communicated with the sample injection channel and a detection channel communicated with the storage channel through a connecting channel, and bacteria microcapsules are stored in thestorage channel; one end of the porous optical fiber probe is inserted into the detection channel, the other end of the porous optical fiber probe is connected with the fluorescence detector; and thelight source is positioned above the multi-channel micro-fluidic chip. Bacterial microcapsules are stored in a channel of the multichannel micro-fluidic chip, a sample can be directly injected into the channel of the multichannel micro-fluidic chip for direct detection, and rapid, sensitive, accurate and reliable detection of biochemical oxygen demand is realized; the problems of short service life, harsh application conditions, poor repeatability and the like of a microbial film in a traditional sensor are solved; the method has the advantages of simplicity, portability, stable detection process and wide application range.

Description

technical field

[0001] The invention relates to the technical field of environmental water sample detection, in particular to a microfluidic detection device and method for biochemical oxygen demand based on bacterial microcapsules. Background technique

[0002] Biochemical oxygen demand (biochemical oxygen demand) is a comprehensive index indicating the content of aerobic pollutants such as organic matter in water. It shows that organic matter in water is oxidized and decomposed due to the biochemical action of microorganisms, making it inorganic or gasified. The total amount of dissolved oxygen. It takes about 20 days in total for all kinds of organic matter in sewage to be completely oxidized and decomposed. In order to shorten the detection time, the general biochemical oxygen demand is represented by the oxygen consumption of the tested water sample within 5 days at 20°C, which is called 5-day biochemical oxygen demand, referred to as BOD 5 . BOD 5 The higher the va...

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