Biochemical oxygen demand microfluidic detection equipment and biochemical oxygen demand microfluidic detection method based on bacterial microcapsules

A biochemical oxygen demand and detection equipment technology, applied in the direction of testing water, measuring devices, material inspection products, etc., can solve the problems of harsh application conditions, poor repeatability, and short microbial film life, and achieve short life, stable performance, Wide range of effects

Active Publication Date: 2021-01-12
TSINGHUA UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

[0006] In view of this, the object of the present invention is to provide a biochemical oxygen demand microfluidic detection device based on bacterial microcapsules, which embeds bacterial microcapsules in a multi-channel microfluidic chip, and can directly inject water samples Direct detection in the multi-channel microfluidic chip realizes rapid, sensitive, accurate and reliable detection of biochemical oxygen demand; overcomes the problems of short microbial film life, harsh application conditions and poor repeatability in traditional sensors

Method used

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  • Biochemical oxygen demand microfluidic detection equipment and biochemical oxygen demand microfluidic detection method based on bacterial microcapsules

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Experimental program
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Effect test

Embodiment 1

[0034] The preparation of embodiment 1 bacillus subtilis microcapsules

[0035] The Bacillus subtilis microcapsules are prepared by a flow focusing method of a microfluidic chip. Specifically, it includes: first culture Bacillus subtilis at 30°C and 170r / min for 24h, then centrifuge at 4°C and 3000r / min for 15 minutes to collect the bacteria, wash twice with phosphate buffer to prepare a 20mg / mL bacterial suspension. Then sodium alginate (concentration can be 4-8%, specifically 4%), polyvinyl alcohol (concentration can be 8-15%, specifically 10%) solution is mixed with 9: 1 ratio, the mixed solution obtained and Equal volumes of bacterial suspensions were mixed, and the bacteria-containing mixed solution obtained again was mixed with an equal volume of 100mM Ca-EDTA as a dispersed phase, wherein sodium alginate and polyvinyl alcohol were used as wall materials, mechanically Higher strength is more conducive to the stability of bacterial microspheres. Next, fluorinated oil FC...

Embodiment 2

[0036] The design of the multi-channel microfluidic chip of embodiment 2 Bacillus subtilis microcapsules

[0037] First, design the specific structure of the multi-channel microfluidic chip; among them, the bottom plate of the chip is made of glass, and each channel is made of polydimethoxysiloxane, polyacrylic acid, etc. Specific structural design: the multi-channel microfluidic chip is set as three main channels, including: storage channel 1 on the left, detection channel 2 in the middle, and storage channel 1 on the right, wherein two storage channels 1 The length is 40mm and the width is 25mm; the detection channel 3 is 40mm long and 50mm wide, and the specific dimensions of the detection channel 2 and the storage channel 1 are sufficient to accommodate enough liquid and optical fiber probes; the height of the storage channel 1 and the detection channel 2 is 800 μm . The storage channel 1 and the detection channel 2 are connected through a thin connection channel 3, the h...

Embodiment 3

[0039] Embodiment 3 The design of the porous fiber optic probe 6 and the installation of the detection equipment

[0040] First, the commercially available porous optical fiber was washed and soaked with 0.1M NaOH for 2 hours, and then the modification sol was inhaled under the condition of 0.08 MPa, that is, after standing at room temperature for 2 hours, the modification was completed, washed with water, and dried for later use. Wherein, the preparation method of the modified sol is: mix 1mg[Ru(dpp) 3 ]Cl 2 Added to 7.5 mL mixed solution (22.7% ethyl orthosilicate, 22.7% Octyl-triEOS, 50% ethanol, 8% HCl).

[0041] Then, take out the multi-channel microfluidic chip and activate it for 16 hours, insert one end of the modified porous fiber optic probe 4 into the detection channel 2, and connect the unmodified end to the fluorescence detector 7 to read the stored value. Wherein, the end of the detection channel, that is, the insertion end of the porous optical fiber probe, is...

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Abstract

The invention discloses biochemical oxygen demand microfluidic detection equipment and a biochemical oxygen demand microfluidic detection method based on bacterial microcapsules. The detection equipment comprises a multi-channel micro-fluidic chip, a porous optical fiber probe, a fluorescence detector and a light source, the multi-channel micro-fluidic chip comprises a sample injection channel, astorage channel communicated with the sample injection channel and a detection channel communicated with the storage channel through a connecting channel, and bacteria microcapsules are stored in thestorage channel; one end of the porous optical fiber probe is inserted into the detection channel, the other end of the porous optical fiber probe is connected with the fluorescence detector; and thelight source is positioned above the multi-channel micro-fluidic chip. Bacterial microcapsules are stored in a channel of the multichannel micro-fluidic chip, a sample can be directly injected into the channel of the multichannel micro-fluidic chip for direct detection, and rapid, sensitive, accurate and reliable detection of biochemical oxygen demand is realized; the problems of short service life, harsh application conditions, poor repeatability and the like of a microbial film in a traditional sensor are solved; the method has the advantages of simplicity, portability, stable detection process and wide application range.

Description

technical field [0001] The invention relates to the technical field of environmental water sample detection, in particular to a microfluidic detection device and method for biochemical oxygen demand based on bacterial microcapsules. Background technique [0002] Biochemical oxygen demand (biochemical oxygen demand) is a comprehensive index indicating the content of aerobic pollutants such as organic matter in water. It shows that organic matter in water is oxidized and decomposed due to the biochemical action of microorganisms, making it inorganic or gasified. The total amount of dissolved oxygen. It takes about 20 days in total for all kinds of organic matter in sewage to be completely oxidized and decomposed. In order to shorten the detection time, the general biochemical oxygen demand is represented by the oxygen consumption of the tested water sample within 5 days at 20°C, which is called 5-day biochemical oxygen demand, referred to as BOD 5 . BOD 5 The higher the va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18G01N21/64
CPCG01N21/6428G01N33/1806G01N33/1866G01N2021/6432
Inventor 林金明张婉玲
Owner TSINGHUA UNIV
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