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Corn polypeptide powder and method for extracting corn polypeptide by ultrasonic-assisted enzymatic method

A corn polypeptide and extraction method technology, applied in fermentation and other directions, can solve the problems of low utilization rate of protease, shorten extraction time, and long enzymolysis time, and achieve the advantages of shortening enzymolysis time, improving enzyme utilization rate and increasing added value Effect

Active Publication Date: 2021-01-19
GUOTOU BIO TECH INVESTMENT CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problems of low product yield, long enzymolysis time and low utilization rate of protease in the traditional protease method existing in the prior art, and provide a method for extracting corn polypeptide by ultrasonic-assisted enzymatic method and the method The extracted corn polypeptide powder, which uses ultrasonic method and carbohydrase-assisted extraction, promotes the pulverization and liquefaction of prolamin, improves the activity of protease molecules, increases the contact probability of prolamin and protease active sites, and improves protease activity. Utilization, significantly improved extraction efficiency and shortened extraction time

Method used

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  • Corn polypeptide powder and method for extracting corn polypeptide by ultrasonic-assisted enzymatic method
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  • Corn polypeptide powder and method for extracting corn polypeptide by ultrasonic-assisted enzymatic method

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Embodiment approach

[0025] According to a preferred embodiment of the present invention, the corn fermented mash contains 3-4% by weight of crude protein.

[0026] According to the present invention, the purpose of the present invention can be achieved as long as ultrasound is introduced in any one of the first enzymolysis and the second enzymolysis step, but more preferably, both the first enzymolysis and the second enzymolysis are performed under ultrasonic under the conditions.

[0027] According to the present invention, preferably, the first enzymatic hydrolysis is carried out under ultrasonic conditions, and the ultrasonic conditions can be changed in a wide range, but in order to further improve the effect of the present invention, the ultrasonic power is 100 -300W, for example, can be 100W, 110W, 120W, 130W, 140W, 160W, 180W, 200W, 220W, 240W, 260W, 280W, 300W, preferably 110-140W.

[0028] According to the present invention, the conditions of the first enzymatic hydrolysis can be select...

Embodiment 1

[0063] This example is used to illustrate the method for extracting corn polypeptide from corn fermented mash provided by the present invention

[0064] (1) Adjust the pH of 100g corn fermented mash to 4.8-5, add starch glucoamylase, cellulase and hemicellulase, wherein the addition of glucoamylase is 0.1% of solid dry weight, and the addition of cellulase is solid dry weight The weight is 0.6%, and the amount of hemicellulase added is 0.1% of the dry weight of the solid. First, it is sonicated at 120W for 2 hours and then enzymatically hydrolyzed at 250rpm for 22h. The temperature is kept at 50°C during the whole process of enzymatic hydrolysis.

[0065] (2) After enzymatic hydrolysis, solid-liquid separation, the solid is washed with pure water for many times until there is no free residual sugar on the surface, the washing water is combined with the liquid phase obtained in step (1) and solid-liquid separation, and the amount of glucose in the liquid phase is measured , and...

Embodiment 2

[0069] This example is used to illustrate the method for extracting corn polypeptide from corn fermented mash provided by the present invention

[0070] (1) Regulate the pH of 100g corn fermented mash to 4.8-5, add starch glucoamylase, cellulase and hemicellulase, wherein the addition of glucoamylase is 0.08% of solid dry weight, and the addition of cellulase is solid dry weight The weight is 0.4%, and the amount of hemicellulase added is 0.3% of the solid dry weight. First, it is sonicated at 110W for 1.5h and then enzymatically hydrolyzed at 200rpm for 25h. The whole process of enzymatic hydrolysis maintains a temperature of 55°C.

[0071] (2) After enzymatic hydrolysis, solid-liquid separation, the solid is washed with pure water for many times until there is no free residual sugar on the surface, the washing water is combined with the liquid phase obtained in step (1) and solid-liquid separation, and the amount of glucose in the liquid phase is measured , and the results a...

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Abstract

The invention relates to a corn polypeptide extraction process, in particular to corn polypeptide powder and a method for extracting corn polypeptide by an ultrasonic-assisted enzymatic method. The method comprises the following steps: (1) a corn fermented mash is in contact with glucoamylase for first enzymolysis, solid-liquid separation is conducted on a first enzymolysis product to obtain a solid-phase enzymolysis product; (2) the solid-phase enzymolysis product is in contact with protease after being mixed with water for second enzymolysis, and second solid-liquid separation is performed on a second enzymolysis product to obtain a hydrolysate rich in polypeptide; and (3) the hydrolysate is heated for enzyme deactivation, cooling is performed, and then, concentrating and drying are performed to obtain the corn polypeptide powder; and the first enzymolysis and / or the second enzymolysis are / is performed under the ultrasonic condition. According to the method, an ultrasonic method andthe glucuronidase are employed for assisting extraction, smashing and liquefaction of prolamin are promoted, the activity of protease molecules is improved, the contact probability of the prolamin andactive sites of the protease is increased, the utilization rate of the protease is increased, the extraction efficiency is remarkably improved, and the extraction time is shortened.

Description

technical field [0001] The invention relates to a corn polypeptide extraction process, in particular to a corn polypeptide powder and a method for extracting corn polypeptides by an ultrasonic-assisted enzymatic method. Background technique [0002] In recent years, with the increasingly serious environmental problems such as coal and oil energy crises, air pollution, and global warming, the production of fuel ethanol as a renewable bioenergy has increased dramatically. my country's biofuel ethanol process mainly uses corn as raw material for production, through liquefaction, simultaneous saccharification and fermentation, and alcohol distillation, accompanied by the production of a large amount of distiller's grains. Distiller's grains mash contains a large amount of crude protein, rich in amino acids, microorganisms and various trace elements. If it is not handled properly, it will cause a huge waste of resources and serious environmental pollution. If it is handled proper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06Y02E50/10
Inventor 林海龙邓立康刘晓峰石飞虹隋中华刘志国从志会高雷范新龙张志凌刘劲松熊强
Owner GUOTOU BIO TECH INVESTMENT CO LTD