Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Perch immunity-related SNP site and application thereof in breeding

A locus and perch technology, applied in the genetic field of molecular breeding, can solve the economic loss of perch and other problems, and achieve the effect of speeding up the genetic breeding process

Active Publication Date: 2021-01-26
HUZHOU TEACHERS COLLEGE
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, gill rot disease caused by flexibacter columnar, aeromonas hydrophila infection, enteritis disease caused by edward's bacteria, and ulcer disease caused by aeromonas hydrophila infection have caused serious damage to perch farming. Economic losses

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Perch immunity-related SNP site and application thereof in breeding
  • Perch immunity-related SNP site and application thereof in breeding
  • Perch immunity-related SNP site and application thereof in breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Screening of SNP sites and primer design related to disease-resistant immunity of perch

[0039] 10 samples were selected from the families and susceptible families that showed better disease resistance against Aeromonas hydrophila infestation, and the specific steps were as follows:

[0040] 1) Extract the genomic DNA of the sample

[0041] Take 0.5 g of perch fin rays to be tested, add 500 μl SDS lysis buffer (50 μl 10% SDS, 5 μl proteinase K (20 mg / ml)), cut into pieces, and then lyse at 56° C. for 3-5 hours until the lysate is clear. Add the same volume of saturated phenol: chloroform / isoamyl, mix for 30 minutes, and then centrifuge at 12000 rpm for 10 minutes. Take the supernatant and repeat the above steps until there is no protein layer between the aqueous phase and the organic phase. Take the supernatant solution, add the same volume of chloroform / isoamyl alcohol, shake gently for 20min, and centrifuge at 12000rpm for 10min. Take the supernatant, ad...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a perch immunity-related SNP site and application thereof in breeding. By comparing CD63 genes of different families, the SNP site related to disease resistance is obtained, anda screening marker is provided for screening disease-resistant perches. The invention firstly provides the perch immunity-related SNP site, the SNP site is located at the 223 site of the perch CD63 gene with the nucleotide sequence of SEQ ID NO: 1, and the basic group of the SNP site is C or A. The invention further provides a PCR amplification or sequencing primer for detecting the SNP site. The perch immunity-related SNP site is obtained through screening, perch individuals with higher resistance to pathogenic bacteria can be screened out by detecting the SNP site, and therefore the genetic breeding process of perches is accelerated.

Description

technical field [0001] The invention belongs to the field of genetic technology of molecular breeding, and more specifically relates to a squid immune-related SNP site and its application in breeding. Background technique [0002] Perch (Lateolabrax japonicus) is a carnivorous wide-salt fish. As a migratory fish in warm-temperate clusters in coastal waters, it is the main economic fish in my country's coastal waters. Bass is mainly distributed in the western Pacific Ocean, the coast of China and freshwater bodies leading to the sea. There are many in the Yellow Sea and Bohai Sea, and it is one of the common economic fish. Bass meat is white and tender, fragrant, without fishy smell, and the meat is in the shape of garlic cloves. It is most suitable for steaming, braised in soy sauce or stewed soup. It is one of the common economic fishes that I have ever lived, and it is also a species for the development of marine aquaculture. [0003] According to statistics, in 2017, t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/6888C12Q1/686C12Q2600/156C12Q2600/124C12Q2527/107
Inventor 朱俊杰刘标刘春冬陆国富
Owner HUZHOU TEACHERS COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com