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Culture medium capable of promoting rapid growth of osmophilic yeast in honey and preparation method thereof

A culture medium and yeast technology, applied in the field of cell culture, can solve the problems of technical inconvenience for detection technicians, long culture cycle, unsuitable detection efficiency, etc., and achieve the effect of shortening the culture time and improving the efficiency of experimental detection

Pending Publication Date: 2021-01-29
GREENTOWN AGRI TESTING TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, when using traditional medium such as DG18 medium to culture osmophilic yeast, the culture period is long, which is not suitable for the improvement of detection efficiency
At the same time, because the color of osmophilic yeast is similar to the color of the medium, it is very inconvenient for detection technicians

Method used

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  • Culture medium capable of promoting rapid growth of osmophilic yeast in honey and preparation method thereof
  • Culture medium capable of promoting rapid growth of osmophilic yeast in honey and preparation method thereof
  • Culture medium capable of promoting rapid growth of osmophilic yeast in honey and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A medium that can promote the rapid growth of osmophilic yeast in honey, the medium includes the following components in terms of mass content: 0.2g of water extract of Feilian, 0.06g of water extract of C. japonica, water extract of Yinjing 0.1g, 0.03g of sugar palm water extract, 0.01g of fenugreek water extract, 1g of potato extract powder, 11g of anhydrous glucose, 0.7g of potassium hydrogen phosphate, 4g of casein peptone, 0.001g of nichloramine, 0.02g of Bengal red , 0.002g of photocatalyst, 15-20g of agar, dissolved in 1000mL of distilled water.

[0029] The preparation method of above-mentioned culture medium comprises:

[0030] (1) Add pure water into the container and heat to boil;

[0031] (2) When the boiling water is cooled to 50°C, take the water extract of Feilian, the water extract of the blue thorn head, the water extract of the silver wattle, the water extract of the sugar palm, the water extract of Dingguicao, the potato extract powder, and the anhyd...

Embodiment 2

[0035] A medium that can promote the rapid growth of osmophilic yeast in honey, the medium includes the following components in terms of mass content: 0.3g of pheasantia water extract, 0.07g of blue thorn water extract, and silver wattle water extract 0.15g, sugar palm water extract 0.04g, Dingguicao water extract 0.03g, potato powder 1.6g, anhydrous glucose 12g, potassium hydrogen phosphate 0.9g, casein peptone 5g, chloramine 0.002g, Bengal red 0.03 g. Photocatalyst 0.003g, agar 15-20g, dilute to 1L with purified water.

[0036] The preparation method of above-mentioned culture medium is the same as embodiment 1.

Embodiment 3

[0038] A medium that can promote the rapid growth of osmophilic yeast in honey, the medium includes the following components in terms of mass content: 0.36g of pheasantia water extract, 0.072g of blue thorn water extract, and silver wattle water extract 0.16g, 0.53g of sugar palm water extract, 0.34g of fenugreek water extract, 1.6g of potato extract powder, 12.3g of anhydrous glucose, 0.9g of potassium hydrogen phosphate, 5.3g of casein peptone, 0.025g of chloramine, Bengal Red 0.034g, photocatalyst 0.003g, agar 15-20g, dilute to 1L with purified water.

[0039] The preparation method of above-mentioned culture medium is the same as embodiment 1.

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Abstract

The invention discloses a culture medium capable of promoting rapid growth of osmophilic yeast in honey and a preparation method thereof, and particularly belongs to the field of cell culture. The culture medium comprises the following components: 0.2-0.4 g of aqueous extract of carduus crispus, 0.06-0.08 g of aqueous extract of echinops latifolius, 0.1-0.2 g of aqueous extract of acacia dealbata,1-2 g of potato extract, 11-13 g ofanhydrous glucose, 0.7-1.4 g ofpotassium hydrogen phosphate, 4-6 g ofcasein peptone, 0.001-0.003 g ofdicloran, 0.02-0.04 g ofrose-bengal, 0.002-0.004 g of a photo-catalyst, and 15-20 g ofagar; and dissolving in 1000 mL of distilled water. The culture medium has the advantage of being capable of promoting rapid growth of osmophilic yeast.

Description

technical field [0001] The invention belongs to the field of cell culture, in particular to a culture medium capable of promoting the rapid growth of osmophilic yeast in honey and a preparation method thereof. Background technique [0002] There are two main types of yeast in honey: one is osmophilic yeast, and the other is high sugar-tolerant yeast. Among them, osmophilic yeast has a greater impact on the quality of honey. Therefore, the new national standard increases the limit requirement of osmophilic yeast count, which is also a need to further improve the safety of honey products. At present, the detection of osmophilic yeast count is mainly carried out according to the method stipulated in Appendix A of GB14963-201l "National Food Safety Standard for Honey". The culture condition for osmophilic yeast in the national standard method is 7 days in a constant temperature incubator at 25 °C ± 1 °C, which is 4 to 5 days longer than the general colony culture period. [00...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 陆雯王珍章虎吴岳琴俞琦娅李秀通彭醒醒田平李文娟
Owner GREENTOWN AGRI TESTING TECH CO LTD
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