High-performance renewable corneal material, reparation method and application thereof

A regenerative, high-performance technology, applied in the field of medical devices, can solve the problems of graft dissolution and long healing time, shortage of donor cornea, scarcity of cornea sources, etc., to achieve the function of promoting wound healing, good degradation resistance and biological Good compatibility and product reproducibility

Active Publication Date: 2021-02-02
济南金泉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to various factors, the source of corneas in eye banks is extremely scarce at present, and many patients can only passively wait for donations
[0005] Corneal transplantation is currently the most effective method for treating corneal blindness, but clinical problems such as severe shortage of donor corneas and postoperative immune rejection are still faced. Therefore, in recent years, researchers have tried to prepare various corneal material substitutes to solve this problem
However, there are still problems such as low transparency, graft dissolution and long healing time after lamellar transplantation of acellular animal corneas.

Method used

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  • High-performance renewable corneal material, reparation method and application thereof
  • High-performance renewable corneal material, reparation method and application thereof
  • High-performance renewable corneal material, reparation method and application thereof

Examples

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preparation example Construction

[0033] In a first aspect, the present invention provides a method for preparing a high-performance regenerative corneal material, the method comprising: performing a preliminary mild decellularization, washing, and cleaning of fresh human or animal eyeball corneal slices cut under aseptic conditions. Remove the decellularization agent, supplement glycosaminoglycan cross-linking, follow up multiple times of thorough decellularization, wash off the decellularization agent again, seal up the preservation solution, and sterilize.

[0034] Wherein, the method of cutting out the corneal stroma of a fresh animal or human eyeball under aseptic conditions includes first soaking the human or fresh animal eyeball with alcohol or a solution containing tobramycin, and then cutting out the corneal stroma under aseptic conditions tablet; the volume percentage concentration of the alcohol is preferably 70%-80%, the concentration of the tobramycin is preferably 30000-50000U / L; the animal is pre...

Embodiment 1

[0048] Take the eyeballs of pigs that have just been killed in the slaughterhouse, rinse them with 75% alcohol until the surface of the eyeballs is free of blood stains, then soak them in 40000U / L tobramycin solution and bring them back;

[0049] Take out the pig’s eyeball in a sterile room, rinse the entire eyeball with 0.9% normal saline, then cut off the fascia muscles around the eyeball, then rinse the entire eyeball with sterile PBS, and finally rinse the entire eyeball with 0.9% normal saline eyeball;

[0050] The cornea was directly drilled from the eyeball with a 5 mm diameter trephine, and the material was cut under a microscope to obtain a corneal material with a thickness of 200 μm that only contained Bowman’s layer and part of the stroma; Shaking treatment at room temperature for a certain period of time, so that the corneal epithelial cell layer and endothelial cell layer are shed and some stromal cells are lysed and killed; wherein, the decellularization agent so...

Embodiment 2

[0061] Take the eyeballs of cattle that have just been executed in the slaughterhouse, rinse them with 75% alcohol until the surface of the eyeballs is free of blood stains, then soak them in 40000U / L tobramycin solution and bring them back;

[0062] Take out the bull’s eyeball in a sterile room, rinse the whole eyeball with 0.9% normal saline, then cut off the fascia muscles around the eyeball, then wash the whole eyeball with sterile PBS, and finally wash the whole eyeball with 0.9% normal saline eyeball;

[0063] The cornea was directly drilled from the eyeball with an 8 mm diameter trephine, and the material was cut under a microscope to obtain a material with a thickness of 400 μm that only contained Bowman's layer and part of the stroma; Shaking treatment for a certain period of time, so that the corneal epithelial cell layer and endothelial cell layer are shed and some stromal cells are lysed and killed; wherein, the decellularization agent solution is 1.5% w / v sodium d...

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Abstract

The invention belongs to the field of medical devices, and relates to a high-performance renewable corneal material, a preparation method and an application thereof. The preparation method of the corneal material comprises the following steps: harvesting fresh lamellar corneas from animal or human eyeballs under aseptic conditions, and sequentially performing preliminary mild decellularization, elution and removal of decellularization agent, supplementation of glycosaminoglycan for crosslinking, subsequent repeated thorough decellularization, secondary elution and removal of the decellularization agent, preservation with preserving liquid and sterilization. According to the invention, the high-performance renewable corneal material prepared by a repeated decellularization technology and akey technology of synergistic modification with efficient healing-promoting materials has high transparency, good mechanical property, good degradation resistance and biocompatibility and can promotehealing of corneal wounds. The high-performance renewable corneal material is a major breakthrough in corneal material preparation technology. Meanwhile, the process of the present invention is simple, flexible, good in product reproducibility and easy to realize industrial popularization. The renewable corneal material of the present invention can be widely used for treating blinding eye diseasescaused by corneal lesions or corneal injuries.

Description

technical field [0001] The invention relates to the field of medical devices, in particular to a high-performance regenerative corneal material and its preparation method and application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The cornea is a layer of transparent membrane located on the anterior wall of the eyeball, accounting for about 1 / 6 of the fibrous membrane. Blood vessels are composed of connective tissue. The thickness of the cornea varies from part to part, with the central part being the thinnest. [0004] Corneal infection, corneal trauma and other diseases may lead to corneal blindness. Among these patients, most people can regain their sight throug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/20A61L27/50
CPCA61L27/3604A61L27/3687A61L27/50A61L27/20A61L2430/16A61L2300/41
Inventor 曹成波王倩庞鵾鹏张春莲孙永强郭安琦
Owner 济南金泉生物科技有限公司
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