Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Separation preparation method of peonidin-3-O-(6-O-p-coumaroyl) glucoside-5-glucoside

A technology of glucoside and coumaroyl, which is applied in the field of separation and purification of natural products, can solve the problems of no acylated anthocyanins, difficulty in separation and purification of acylated anthocyanins, and difficulty in obtaining target anthocyanins, and achieves good repeatability and processing efficiency. large amount of effect

Active Publication Date: 2021-02-02
ZHEJIANG UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this technical scheme discloses its separation and obtains two kinds of anthocyanins, but from its figure 1 In the HPLC chart of the turbid grape juice, it can be seen that its anthocyanin composition is simple. From the limitation of high-speed countercurrent chromatography, it can be inferred that when the anthocyanin components of the separated sample are complex, it may be difficult to obtain the target anthocyanin by this method
[0008] Due to the difficulty in the separation and purification of acylated anthocyanins, there is currently no commercial standard acylated anthocyanins on the market

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Separation preparation method of peonidin-3-O-(6-O-p-coumaroyl) glucoside-5-glucoside
  • Separation preparation method of peonidin-3-O-(6-O-p-coumaroyl) glucoside-5-glucoside
  • Separation preparation method of peonidin-3-O-(6-O-p-coumaroyl) glucoside-5-glucoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Wash and peel the grapes to obtain 1 kg of grape skins, add 70% ethanol solution containing 0.5% (v / v) hydrochloric acid according to the ratio of solid to liquid 1g: 8mL, mix well, and ultrasonically extract for 60 minutes, (control the temperature below 50°C , protected from light), filtered through gauze, the filtrate was centrifuged at 4000rpm for 10min, and the supernatant was taken. The filter residue was extracted once more in the same way. The filtrates were combined and filtered one more time using a Buchner funnel. The filtrate was evaporated under reduced pressure at 40°C to remove ethanol and concentrated to obtain a crude grape skin anthocyanin extract. The high performance liquid chromatogram of grape skin anthocyanin crude extract is as follows figure 2 shown.

[0048] Put the D101 macroporous resin into the chromatographic column, and use ethanol, 0.5mol / L hydrochloric acid solution, 0.5mol / L sodium hydroxide solution, and water to wash in sequence, ...

Embodiment 2

[0052] Wash and peel the grapes to obtain 5kg of grape skins, add 70% ethanol solution containing 0.5% (v / v) hydrochloric acid according to the ratio of solid to liquid 1g: 6mL and mix thoroughly, ultrasonically extract for 90min, (control the temperature below 50°C , protected from light), filtered through gauze, the filtrate was centrifuged at 4000rpm for 10min, and the supernatant was taken. The filter residue was extracted once more in the same way. The filtrates were combined and filtered one more time using a Buchner funnel. The filtrate was evaporated under reduced pressure at 40°C to remove ethanol and concentrated to obtain a crude grape skin anthocyanin extract.

[0053] Put the AB-8 macroporous resin into the chromatographic column, and use ethanol, 0.5mol / L hydrochloric acid solution, 0.5mol / L sodium hydroxide solution, and water to wash in sequence, and then wash the crude anthocyanin extract at 0.2BV / The flow rate of h is injected into the chromatography colum...

Embodiment 3

[0057] Wash and peel the grapes to obtain 10kg of grape skins, add 60% ethanol solution containing 0.5% (v / v) hydrochloric acid according to the ratio of solid to liquid 1g: 5mL, fully mix, and ultrasonically extract for 60min, (control the temperature below 50°C , protected from light), filtered through gauze, the filtrate was centrifuged at 4000rpm for 10min, and the supernatant was taken. The filter residue was extracted once more in the same way. The filtrates were combined and filtered one more time using a Buchner funnel. The filtrate was evaporated under reduced pressure at 40°C to remove ethanol and concentrated to obtain a crude grape skin anthocyanin extract.

[0058] Put the AB-8 macroporous resin into the chromatographic column, and use ethanol, 0.5mol / L hydrochloric acid solution, 0.5mol / L sodium hydroxide solution, and water to wash in sequence, and then wash the crude anthocyanin extract at 0.2BV / The flow rate of h is injected into the chromatography column. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a separation preparation method of peonidin-3-O-(6-O-p-coumaroyl) glucoside-5-glucoside. By the steps of extraction, macroporous resin purification, extraction, preparation bya liquid chromatogram and a high-speed countercurrent chromatogram and the like, separation and purification are carried out from grapes to obtain a high-purity anthocyanin monomer. By the method, atleast 50mg of peonidin-3-O-(6-O-p-coumaroyl) glucoside-5-glucoside can be obtained from 10kg of grape skin; and the purity can be not lower than 98%. The method has the advantages of simplicity for operation, large treatment capacity, good repeatability and the like, and provides a new idea for development and utilization of grape resources in China.

Description

technical field [0001] The invention relates to the technical field of separation and purification of natural products, in particular to a method for the separation and preparation of paeoniflorin-3-O-(6-O-p-coumaroyl)glucoside-5-glucoside. Background technique [0002] Grapes are the fruit of the plant Vitis vinifera and are cultivated all over the world. Grapes have high nutritional value. Chinese medicine believes that grapes are flat in nature and sweet in taste, can nourish qi and blood, strengthen the heart and diuresis. Modern research shows that grapes not only contain vitamin B1, vitamin B2, vitamin C, vitamin E and other vitamins, but also rich polyphenolic compounds such as resveratrol, proanthocyanidins, anthocyanins, etc. Tumor, antibacterial and other biological activities. [0003] Anthocyanins are one of the important polyphenols in grapes. Studies have shown that grapes contain anthocyanins formed by combining anthocyanins such as delphinidin, mallowin, p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H17/06C07H1/08
CPCC07H1/08C07H17/06
Inventor 陈卫谢佳宏徐阳崔昊昕
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com