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Red blood cell average life detection method based on HbA1c and blood glucose concentration as sensing variables

A technology of blood glucose concentration and average lifespan, applied in biological testing, material inspection products, etc., can solve the problems of complex processing, processing time of more than 1 day, and complicated operation.

Active Publication Date: 2021-02-02
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CO measurement can be achieved by gas chromatography (Ma, Jie; Dou, Yanna; Zhang, Hanjie; et al., Blood Purification, 2017, 43, 200-205) or non-dispersive infrared spectroscopy (CN209529179U). Compared with this method, the convenience of this method has been greatly improved, but this method is complicated to operate, the subject experience is poor, and the sample pretreatment is complicated, and the processing time exceeds 1 day; it is especially vulnerable to smoking, obesity, weight, myocardial infarction, muscle effects of necrosis

Method used

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  • Red blood cell average life detection method based on HbA1c and blood glucose concentration as sensing variables
  • Red blood cell average life detection method based on HbA1c and blood glucose concentration as sensing variables
  • Red blood cell average life detection method based on HbA1c and blood glucose concentration as sensing variables

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Embodiment 1

[0119] an HbA-based lc A method for detecting the mean life span of erythrocytes with blood glucose concentration as the sensing variable, using HPLC technology to measure the patient's A HbA 1c = 8.4%, measured using the CGM blood glucose monitoring system [AG D ]=140mg / dL, the mean life expectancy M of the patient's erythrocytes is calculated by equation (34) or (35) or (36), (37) and (38), respectively RBCl = 176 days, mean current age of red blood cells M RBCa = 88 days and mean residual age of red blood cells M RBCa = 88 days.

Embodiment 2

[0121] an HbA-based 1c A method for detecting the average lifespan of red blood cells with blood glucose concentration sensing, using capillary electrophoresis to measure the patient's BHbA 1c = 4.2%, measured with a blood glucose meter [G FBG ]=163 mg / dL, the mean life span M of the patient’s erythrocytes is calculated by equation (34) or (35) or (36), (37) and (38), respectively RBCl = 68 days, mean current age of red blood cells M RBCa = 34 days and mean residual age of red blood cells M RBCra = 34 days.

Embodiment 3

[0123] an HbA-based lc A method for detecting the average lifespan of red blood cells with blood glucose concentration sensing, using isoelectric focusing technology to measure the patient's CHbA 1c = 6.5%, using a blood glucose meter to measure the fasting blood glucose concentration and the blood glucose concentration measured 0.5, 1, 1.5, and 2 hours after oral administration of 75 g of glucose, and the average blood glucose concentration obtained five times is [G OGTT ]=244mg / dL, the mean life span M of the patient's erythrocytes is calculated by equation (34) or (35) or (36), (37) and (38), respectively RBCl = 96 days, mean current age of red blood cells M RBCa = 48 days and mean residual age of red blood cells M RBCra = 48 days.

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Abstract

The invention relates to a red blood cell average life detection method based on HbA1c and blood glucose concentration as sensing variables, which comprises the following steps: (1) measuring the concentration HbA1c (%) and the blood glucose concentration [G] of HbA1c in blood sample blood, (2) substituting the measured HbA1c (%) and blood glucose concentration [G] into a formula to calculate thered blood cell average life MRBCl,wherein MRBCl is the average life of red blood cells in a blood sample, HbA1c (%) is the concentration of glycosylated hemoglobin in blood, [G] is the concentration of blood glucose, and K is a correction coefficient. Compared with the prior art, the method provided by the invention has the advantages of simple operation, time and labor saving, low cost, low sample demand, avoidance of potential radioactive exposure, infection and allergic harm, avoidance of numerous influence interferences and the like, and has wider adaptability.

Description

technical field [0001] The invention belongs to the technical field of analysis of the average lifespan of human red blood cells, and in particular relates to an HbA-based 1c and blood glucose concentration as a sensing variable for the detection of the mean lifespan of red blood cells. Background technique [0002] The determination of the average life span of red blood cells plays an important role in the diagnosis and differential diagnosis of major diseases such as ischemic anemia, hereditary anemia, drug-induced hemolysis, allergic hemolysis, hereditary hemolysis, internal bleeding and diabetes. Such as hereditary hemolysis and hereditary hemoglobinopathies, the red blood cell life span is significantly shorter than that of normal people (Higgins P J, Bunn HF. J. Biol. Chem., 1981, 256, 5204-5208). Hypersplenism, chronic internal hemorrhage in tissues and organs, increased blood viscosity, mitral valve stenosis, and increased vascular stiffness can all lead to decrease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
CPCG01N33/48
Inventor 曹成喜田佑吏查根晗李红根王宇兴刘伟文张强刘小平
Owner SHANGHAI JIAO TONG UNIV
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