Unlock instant, AI-driven research and patent intelligence for your innovation.

Antitumor protein extract in Pleurotus ferulae lenzi as well as preparation method and application of antitumor protein extract

A technology of protein extract and ferulae ferulae, applied in the biological field, can solve problems that have not been seen yet, and achieve good safety, good anti-tumor effect, and low cost effect

Active Publication Date: 2021-02-05
NANKAI UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, most of the current researches on the bioactive substances of ferulae ferulae focus on polysaccharides, but less on proteins, Zheng Jie [20] et al. extracted polyphenol oxidase (PPO) from Ferulicum ferulae, and briefly determined its physical and chemical properties, and the lectin protein isolated from Ferulicia japonica with a size of about 17.5KD can significantly promote the growth rate of mice. The proliferation of lymphocytes enhances its immune activity, but there is no relevant report on the extraction of anti-tumor proteins from Ferula aeruginosa

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antitumor protein extract in Pleurotus ferulae lenzi as well as preparation method and application of antitumor protein extract
  • Antitumor protein extract in Pleurotus ferulae lenzi as well as preparation method and application of antitumor protein extract
  • Antitumor protein extract in Pleurotus ferulae lenzi as well as preparation method and application of antitumor protein extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation and detection of anti-tumor protein extract from Pleurotusferulaelenzi.

[0030] (1) Dried the fruiting bodies of Ferulae ferulae at 55°C and ground them into powder, soaked in distilled water 10 times the weight of the powder, leached at 22°C for 12 hours, centrifuged at 4000rpm for 20min, and collected the supernatant;

[0031] (2) Add ammonium sulfate to the supernatant until the saturation is 80%, place it at 4°C for 12 hours, centrifuge at 10,000rpm for 20min, collect the precipitate, and obtain the crude extract of Ferulicum ferulae protein;

[0032] (3) Distilled water dissolves the crude extract of Ferula ferulae protein, then dialyzes in distilled water with 5 times the weight at 2°C for 72 hours, replaces the distilled water every 4 hours, centrifuges at 14000rpm for 15min after dialysis to collect the supernatant and freeze-dries it. That is, ferulae ferulae protein coarse powder;

[0033](4) Dissolve ferulae ferulae protein coarse powder in the ...

Embodiment 2

[0037] Preparation and detection of anti-tumor protein extract from Pleurotusferulaelenzi.

[0038] (1) Dried the fruiting bodies of Ferulae ferulae at 50°C and ground them into powder, soaked in distilled water 10 times the weight of the powder, leached at 23°C for 8 hours, centrifuged at 8000rpm for 10min, and collected the supernatant;

[0039] (2) Add ammonium sulfate to the supernatant until the saturation is 80%, place it at 2°C for 16 hours, centrifuge at 12000rpm for 15min, collect the precipitate, and obtain the crude protein extract of ferulae ferulae;

[0040] (3) Distilled water dissolves the crude extract of Ferula ferulae protein, then dialyzes in distilled water of 10 times the weight at 6°C for 24 hours, replaces the distilled water every 4 hours, centrifuges at 12000rpm for 20min after dialysis to collect the supernatant and freeze-dries it. That is, ferulae ferulae protein coarse powder;

[0041] (4) Dissolve ferulae ferulae protein coarse powder in the Tris...

Embodiment 3

[0045] Preparation and detection of anti-tumor protein extract from Pleurotusferulaelenzi.

[0046] (1) Dried the fruiting bodies of Ferulae ferulae at 70°C and ground them into powder, soaked in distilled water 10 times the weight of the powder, leached at 18°C ​​for 16 hours, centrifuged at 5000rpm for 15min, and collected the supernatant;

[0047] (2) Add ammonium sulfate to the supernatant until the saturation is 80%, place it at 6° C. for 8 hours, centrifuge at 8000 rpm for 25 minutes, collect the precipitate, and obtain the crude protein extract of ferulae ferulae;

[0048] (3) Distilled water dissolves the crude extract of Ferula ferulae protein, then dialyzes in distilled water of 8 times the weight at 4°C for 48 hours, replaces the distilled water every 4 hours, centrifuges at 10,000rpm for 25min after dialysis to collect the supernatant and freeze-dries it. That is, ferulae ferulae protein coarse powder;

[0049] (4) Dissolve ferulae ferulae protein coarse powder in...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses an antitumor protein extract in Pleurotus ferulae lenzi as well as a preparation method and application of the antitumor protein extract. The antitumor protein extract is obtained by drying Pleurotus ferulae lenzi sporocarp, grinding the dried Pleurotus ferulae lenzi sporocarp into powder and carrying out extraction, salting out, dialysis, freeze-drying and ion exchange. The protein extract is named as XD1 and is monomeric protein with the molecular weight of about 17.5 kDa; and the protein extract has an obvious inhibiting effect on the growth of a human non-small celllung adenocarcinoma cell strain A549. The antitumor protein extract in the Pleurotus ferulae lenzi is a natural active product, and has no toxic or side effect on human beings and no pollution to theenvironment; and the preparation process is simple, easy to obtain, lower in cost and suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an anti-tumor protein extract of ferulae ferulae Pleurotusferulaelenzi and a preparation method thereof. Background technique [0002] In recent years, tumor, as one of the most difficult diseases to cure, has seriously affected human health and quality of life, and its incidence is increasing year by year. The methods used in clinical treatment have gone through a long period of exploration. Traditional surgical resection, radiotherapy, and chemotherapy have shortcomings such as limitations, poor selectivity, easy recurrence, and easy metastasis, and it is difficult to completely remove tumor cells. Therefore, the development of It is imminent to develop drugs to prevent tumors. Compared with synthetic chemical drugs, natural anti-tumor drugs have the advantages of low toxicity and high safety. Large edible and medicinal fungi have become an important source of anti-tum...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/18C07K1/30A61K38/02A61P35/00
CPCC07K1/145C07K1/303C07K1/18A61P35/00A61K38/00Y02A50/30
Inventor 刘方刘梦函
Owner NANKAI UNIV