Phenylalanine ammonia lyase gene ThPAL derived from radix tetrastigme and application thereof

A technology of phenylalanine ammonia lyase and gene, which is applied in the field of phenylalanine ammonia lyase gene ThPAL and its application, can solve the problem of relatively little research at the molecular level
CN112359051AActive Publication Date: 2021-02-12杭州鑫旺途科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
杭州鑫旺途科技有限公司
Publication Date
2021-02-12

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Abstract

The invention discloses a phenylalanine ammonia lyase gene ThPAL derived from radix tetrastigme and an application thereof. The nucleotide sequence of the phenylalanine ammonia lyase gene ThPAL is shown as SEQ ID NO.1. According to the invention, radix tetrastigme is used as a biological source to design a primer to amplify the cDNA sequence of radix tetrastigme to obtain the phenylalanine ammonialyase gene ThPAL, and the gene is used as one of key enzymes in a phenylpropane metabolic pathway and can be used for producing resveratrol.
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Description

technical field

[0001] The invention relates to the technical field of genetic engineering, and mainly relates to a phenylalanine ammonia lyase gene ThPAL derived from cloverleaf and application thereof. Background technique

[0002] As the state attaches great importance to traditional Chinese medicine in recent years, the government's strong support has driven the rapid development of the traditional Chinese medicine industry and expanded the market demand for Tetrastigma hemsleyanum Diels et Gilg. The continuous in-depth research on the anti-tumor effect of cloverleaf has led to a rapid increase in the market price of cloverleaf in recent years, and its market demand is also increasing.

[0003] In recent years, the gene regulation of the synthesis of secondary metabolites in medicinal plants has become a very active frontier research field in molecular biology. The amount and composition of metabolites mainly depend on the key enzymes of biosynthesis and their expression...

Claims

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