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Radix scrophulariae iridoid reference extract and preparation method and application thereof

A ginseng iridoid and extract technology, which is applied in the field of scrophulariid iridoid control extract and its preparation, and achieves the effects of less impurities, high transfer rate and good reproducibility

Active Publication Date: 2021-02-12
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Harpagoside with higher purity can be obtained in this patent document, but only involves a kind of purification method of harpagoside, and does not involve the purification methods of harpagoside, angroside C and harpagoside at the same time

Method used

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  • Radix scrophulariae iridoid reference extract and preparation method and application thereof
  • Radix scrophulariae iridoid reference extract and preparation method and application thereof
  • Radix scrophulariae iridoid reference extract and preparation method and application thereof

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preparation example Construction

[0033]The preparation method of the Scrophulariaceae iridoid reference extract of the present invention comprises the following steps: 1) the preparation step of the Scrophulariaceae crude extract; 2) the preparation step of the Scrophulariaceae iridoid reference extract I; 3) the Scrophulariaceae iridoid reference extract I preparation step; Preparation steps of iridoid reference extract II; 4) Preparation steps of reference iridoid extract of Scrophulariaceae iridoids.

[0034]

[0035] (a) Reflux extracting the Scrophulariaceae medicinal material with water as a solvent for 2 to 3 times, each time for 1 to 2 hours, to obtain reflux extracts respectively. Preferably, reflux extraction is performed twice, each time for 1-1.5 hours. The water consumption each time is 9-12 times, preferably 9-11 times, more preferably 10-11 times the weight of the Scrophulariaceae medicinal material. The refluxing extracts are combined and concentrated until the relative density is 1.18-1.22...

preparation example 1

[0080] Preparation Example 1-Preparation of Scrophulariaceae Crude Extract

[0081] Adopt water as solvent, heat reflux extraction method to extract 100g of Radix Scrophulariae medical material, the extraction frequency is 2 times, the water consumption of each extraction is 10 times of the weight of Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Scrophularis medicinal material for each extraction time, and each extraction time is 1 hour, merging the reflux extract, reducing Concentrate under pressure to a relative density of 1.20 to obtain the water extract of Scrophularia scrophulariae.

[0082] Add 95 vol% ethanol to the water extract of Scrophulariaceae until the ethanol concentration is 80 vol%, refrigerate at 4°C for 24 hours, filter with suction, concentrate under reduced pressure, and dry to...

Embodiment 1

[0084] The preparation of embodiment 1-Scrophulariaceae iridoid reference extract I

[0085] The crude extract of Scrophulariaceae in Preparation Example 1 was ultrasonically dissolved with 50 times the mass of methanol, and centrifuged to obtain a mother liquor, which was concentrated to obtain a methanol solution of 0.1 g / mL crude extract of Scrophulariaceae, and the crude extract of Scrophulariaceae was extracted Mix the methanol solution of the substance with the blank silica gel at a mass ratio of 1:2 to obtain the first mixed sample silica gel.

[0086] The first mixed sample silica gel is loaded on the first silica gel column with a diameter-to-height ratio of 1:15 (the crude extract of Scrophulariaceae in the first mixed sample silica gel is 3g, and the mass ratio of blank silica gel used in the first silica gel column is 1:50), and then eluted with the first eluent of 3.0BV to obtain the first silica gel column after elution; wherein, the first eluent is dichloro di...

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Abstract

The invention discloses a radix scrophulariae iridoid reference extract and a preparation method and application thereof. The preparation method comprises the following steps: (1) loading a scrophularia ningpoensis crude extract on a first silica gel column and eluting to obtain an eluted first silica gel column; (2) equally dividing the eluted first silica gel column into N sections along the direction from the column head to the bottom; obtaining a first target section and a second target section; respectively treating to obtain a scrophularia ningpoensis iridoid reference extract I and a scrophularia ningpoensis iridoid reference extract intermediate; (3) treating the scrophularia ningpoensis iridoid reference extract intermediate to obtain a scrophularia ningpoensis iridoid reference extract II; and (4) mixing the scrophularia ningpoensis iridoid reference extract I and the scrophularia ningpoensis iridoid reference extract II to obtain the scrophularia ningpoensis iridoid reference extract. In the radix scrophulariae iridoid reference extract obtained by the invention, the total content of harpagoside, anagloside C and harpagoside is higher than 60wt%.

Description

technical field [0001] The invention relates to a reference extract of Scrophulariaceae iridoids and its preparation method and application. Background technique [0002] Scrophulariaceae was first recorded in "Shen Nong's Materia Medica", and it is one of the "Eight Flavors of Zhejiang". It is a commonly used traditional Chinese medicine with pharmacological activities such as protecting the cardiovascular system, anti-inflammatory, and anti-oxidation. Phenylpropanoid ingredients. [0003] Chinese medicine reference extracts refer to traditional Chinese medicine extracts with clear main chemical components and content, good stability and high purity. The proportion of each chemical component is relatively fixed, and the content of index components has been calibrated, which can realize the qualitative analysis of multiple components at the same time And quantitative analysis, its price is usually lower than the sum of multiple component monomer reference substances. The m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06
CPCG01N30/06Y02A50/30
Inventor 刘斌姜艳艳蒋丽娟
Owner BEIJING UNIV OF CHINESE MEDICINE
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