Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Capture chip for neuroblastoma circulating tumor cells and manufacturing method thereof

A technology for neuroblastoma and tumor cells, which is applied in the field of capture chips for circulating tumor cells of neuroblastoma, can solve the problems of immaturity, complicated operation and low specificity of CTCs detection technology, and achieves improved separation efficiency and purity, The effect of improving the viability of isolated cells, improving the separation efficiency and separation purity

Active Publication Date: 2021-02-23
SHENZHEN CHILDRENS HOSPITAL
View PDF11 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to assist the diagnosis of neuroblastoma and overcome the problems of complicated operation, low specificity and immaturity of the current CTCs detection technology, the present invention provides a capture chip for neuroblastoma CTCs and its production method and capture method; the present invention The technical solutions provided are as follows

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Capture chip for neuroblastoma circulating tumor cells and manufacturing method thereof
  • Capture chip for neuroblastoma circulating tumor cells and manufacturing method thereof
  • Capture chip for neuroblastoma circulating tumor cells and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0044] First: Creating a capture chip for neuroblastoma cells

[0045] A method for making a capture chip for neuroblastoma cells, please refer to Figure 4 The flow chart of this manufacturing method comprises the following steps:

[0046] 1) Using standard photolithography and soft lithography techniques, a microfluidic chip template that can trap neuroblastoma cells is fabricated: using a photomask, patterning is made on a silicon substrate, and then poured on the patterned silicon substrate Add polydimethylsiloxane (PDMS) prepolymer (selected from Momentive RTV615, where glue A and glue B are mixed at a ratio of 10:1) and heat and cure at 80°C for 0.5 hours;

[0047] 2) Peel off the PDMS chip cured in step 1) from the silicon substrate, cut it flat, and punch holes to form inlet holes and outlet holes, such as figure 1 shown;

[0048] 3) Take a glass substrate, after cleaning, carry out oxygen plasma surface activation modification on the contact surface of the glass su...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention relates to a capture chip of neuroblastoma circulating tumor cells (CTCs) and a manufacturing method thereof. The capture chip of the neuroblastoma CTCs comprises a glass substrate layerand a polymer layer which is provided with a microcolumn array pattern, and the surface of the glass substrate layer is modified with a CD56 antibody capable of specifically capturing the CTCs. The method has the beneficial effects that 1, the separation flux is improved; 2, the separation efficiency and purity are improved; 3, CTC in-situ capture is achieved; 4, the activity of separated cells is improved; and 5, the cost is reduced, manual labor and consumable use are reduced through integrated operation, and the detection cost is remarkably reduced.

Description

technical field [0001] The invention relates to a capture chip for circulating tumor cells (CTCs), in particular to a capture chip for neuroblastoma circulating tumor cells (CTCs) and a capture method thereof. Background technique [0002] Neuroblastoma (Neuroblastoma, NB) is the most common extracranial tumor in children, and is the most common tumor in infants. Nearly half of neuroblastoma occurs in infants under 2 years old. It is a cancer that develops from immature nerve cells in various parts of the body, usually from the sympathetic nerves in the abdomen or chest, and most often from the adrenal glands. Patients with different disease origins and spreading sites will show different symptoms, such as abdominal distension, abdominal pain, constipation or diarrhea, dyspnea, skin lumps, bone pain, fatigue, etc. The misdiagnosis rate of neuroblastoma is high, and it is easily misdiagnosed as leukemia, growing pains, rheumatoid arthritis, pneumonia and diarrheal diseases. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00B01L3/00C12N5/09G01N33/569
CPCC12M23/16C12M47/04B01L3/502707B01L3/502761C12N5/0693C12N5/0618G01N33/56966B01L2200/0668
Inventor 文飞球陈小文袁秀丽宋建明刘宗彬
Owner SHENZHEN CHILDRENS HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com