Kit used for detecting brain glioma gene mutation, and preparation method

Abstract

The invention belongs to the biotechnology field, and relates to a cerebrospinal fluid liquid state biopsy technology and a kit preparation method thereof, in particular to a detection kit for dynamically monitoring change of ctDNA in the cerebrospinal fluid of a glioma patient through detection of hotspot gene mutation, and a preparation method of the detection kit. The invention mainly takes brain glioma and meningioma as detection objects, on the basis of a high-throughput gene sequencing detection method, the change of the ctDNA in the cerebrospinal fluid of the glioma patient and a meningioma patient, and main mutation genes related to the glioma and the meningioma are screened to establish a liquid state biopsy gene kit. Through dynamic monitoring of the gene change of the glioma patient before / after an operation, radiotherapy and chemotherapy, a gene-oriented glioma molecular diagnosis, including minimal residual disease surgical excision, pseudoprogression or recurrence, is carried out, and reaction for the radiotherapy and the chemotherapy and change of drug tolerance are monitored.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a cerebrospinal fluid liquid biopsy technique and a preparation method thereof, in particular to a detection kit and a preparation method for dynamically monitoring ctDNA changes in the cerebrospinal fluid of glioma patients by detecting hotspot gene mutations. Background technique [0002] The prior art discloses advances in dynamic, low-invasive liquid biopsy detection of circulating DNA, RNA, or circulating tumor cells of tumor origin, e.g. Rhodes et al. (1994) detected a tumor in the cerebrospinal fluid of a patient with glioma Specific P53 gene mutation. Yuxuan Wang et al. (2015) performed targeted sequencing or whole-genome sequencing on 35 cases of primary central nervous system malignancies, and found that all medulloblastoma, ependymoma, and high-grade glioma near the cerebrospinal fluid space could be detected. at least one mutation. Changcun Pan et al. (2018) Chine...

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Problems solved by technology

Clinical research practice shows that spinal cord dissemination mostly occurs in the late stage of the disease. Once spinal cord dissemination occurs, the prognosis of patients is poor. At present, leptomeningospinal metastasis is mainly based on imaging examination. Less likely, there is no clear molecular spectrum and better clinical cytology pathological diagnosis method