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Ilex chinensis sims essential oil/lycium barbarum polysaccharides antibacterial nanofiber as well as preparation method and application thereof

A technology of wintergreen essential oil and Lycium barbarum polysaccharide, which is applied in fiber treatment, nanotechnology, fiber chemical characteristics, etc., can solve unrelated problems and achieve the effect of good uniformity, natural safety and compatibility

Active Publication Date: 2021-02-23
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are some domestic patent applications about Lycium barbarum polysaccharides. China CN110558320A discloses a compound antibacterial agent of bis-histidine / Lycium barbarum polysaccharide and its preparation method and application; Preparation method and application; Chinese patent CN109876154A discloses the preparation of a class of nanoparticles modified by Lycium barbarum polysaccharide and its anti-tumor activity. However, the patent on the preparation of antibacterial nanofibers by electrospinning technology using Lycium barbarum polysaccharide as a substrate has not yet been involved

Method used

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  • Ilex chinensis sims essential oil/lycium barbarum polysaccharides antibacterial nanofiber as well as preparation method and application thereof
  • Ilex chinensis sims essential oil/lycium barbarum polysaccharides antibacterial nanofiber as well as preparation method and application thereof
  • Ilex chinensis sims essential oil/lycium barbarum polysaccharides antibacterial nanofiber as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 Preparation of wintergreen essential oil / lycium barbarum polysaccharide antibacterial nanofiber

[0022] Preparation method of wintergreen essential oil / lycium barbarum polysaccharide antibacterial nanofiber

[0023] ① Dissolve wintergreen essential oil in absolute ethanol at a ratio of 1:10 (w / v), that is, dissolve 1 mg of essential oil in 10 μL of ethanol, and set aside;

[0024] ② Dissolve β-lactoglobulin (8 μmol / L) in phosphate buffered saline solution (PBS, pH=6.0), and stir magnetically for 5 hours.

[0025] ③ Slowly drop the wintergreen essential oil solution into the β-lactoglobulin solution under stirring conditions, the final concentration of wintergreen essential oil in the solution is 3mg / mL, vortex for 2min, and then continue magnetic stirring for 1h.

[0026] ④ According to the total volume, add polyethylene oxide (35 mg / mL) and magnetically stir for 4 hours, and finally add Lycium barbarum polysaccharide (6 mg / mL) and magnetically stir overn...

Embodiment 2

[0028] The characterization of embodiment 2 wintergreen essential oil / lycium barbarum polysaccharide antibacterial nanofiber

[0029] 1 experimental equipment

[0030] Scanning Electron Microscope COXEM EM30PLUS Beijing Tianyao Technology Co., Ltd.

[0031] 2. Experimental method

[0032] The morphology of the nanofibers was examined using a COXEM EM30PLUS scanning electron microscope. The different samples were sputtered with Au / Pd mixtures under vacuum, and all SEM experiments were performed at 15 kV.

[0033] 3. SEM experimental results

[0034] Scanning electron microscope picture as figure 1 As shown, the blank pure polyethylene oxide nanofibers (above) and wintergreen essential oil / Lycium barbarum polysaccharide antibacterial nanofibers (below B) both have good filamentous properties. Compared with pure polyethylene oxide nanofibers, adding The silk-forming property of the fiber added with wintergreen essential oil / lycium barbarum polysaccharide is not as good as th...

Embodiment 3

[0035] Example 3 Determination of anti-Escherichia coli activity of wintergreen essential oil / lycium barbarum polysaccharide antibacterial nanofibers

[0036] 1 Experimental materials

[0037] Antibacterial agent: pure polyethylene oxide fiber;

[0038] Wintergreen essential oil antibacterial nanofibers;

[0039] Wintergreen essential oil / Lycium barbarum polysaccharide antibacterial nanofiber

[0040] Model species: Escherichia coli

[0041] 2 Experimental methods

[0042] 1) Electrospinning fiber membrane treatment

[0043] Nanofibers were prepared according to Example 1. The antibacterial nanofibers of wintergreen essential oil were nanofibers without Lycium barbarum polysaccharide added to the base material, and dried in an oven for 4 hours. The film was cut into a size of 2cm×2cm and sterilized under ultraviolet light for 2h for later use.

[0044] 2) Experimental determination

[0045] Add 15mL phosphate buffer saline to the 50mL centrifuge tube for sterilization, ...

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Abstract

The invention belongs to the field of food fresh-keeping packaging materials, and particularly relates to a preparation method and application of an ilex chinensis sims essential oil / lycium barbarum polysaccharides antibacterial nanofiber. The preparation method comprises the following steps of embedding ilex chinensis sims essential oil by beta-lactoglobulin to prepare nanoparticles, and then preparing the ilex chinensis sims essential oil / lycium barbarum polysaccharides antibacterial nanofiber by taking lycium barbarum polysaccharides as a substrate and using an electrostatic spinning technology. The preparation method provided by the invention is simple to operate, volatile and unstable essential oil is added into a 'polymer', the essential oil can be protected from being influenced byenvironment, processing and food conditions, and the ilex chinensis sims essential oil / lycium barbarum polysaccharides antibacterial nanofiber can be widely applied to the field of food packaging materials.

Description

technical field [0001] The invention belongs to the field of food fresh-keeping packaging materials, and in particular relates to a wintergreen essential oil / lycium barbarum polysaccharide antibacterial nanofiber, a preparation method and application thereof. Background technique [0002] Ilex (Ilex chinensis Sims) is an evergreen tree belonging to the Ilex family, a class of flowering plants widely distributed in Asia, Europe, northern Africa, North America and South America. In China, it is mainly distributed in the provinces and regions south of the Yangtze River Basin. Its essential oil is widely used in perfumery and aromatherapy. Its main ingredients are safflower seed oil, menthol, blue eucalyptus leaf oil, white pearl leaf oil, etc. Wintergreen essential oil has antibacterial, anti-inflammatory, analgesic and other effects, and can promote local blood circulation. [0003] However, wintergreen essential oil is volatile and unstable when exposed to the air. Therefor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F8/16D01F8/18D01F1/10D01D5/00D04H1/728B82Y40/00
CPCD01F8/16D01F8/18D01F1/103D04H1/728D01D5/003D01D5/0084B82Y40/00Y02A50/30
Inventor 崔海英李虹林琳
Owner JIANGSU UNIV
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