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Optimization method of black carrot pigment extraction process

A technology of black carrot pigment and extraction process, which is applied in the optimization field of black carrot pigment extraction process, and achieves the effect of broad market prospect, less consumption of organic solvent and high extraction efficiency

Inactive Publication Date: 2021-02-26
南京泽朗医药技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cellulase-assisted extraction method has the advantages of high extraction efficiency, fast speed, and simple processing. It has been widely used in the extraction process of natural substances such as alkaloids, flavonoids, and polysaccharides. However, there is still a certain gap in the research on the extraction of pigments.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for optimizing the extraction process of black carotene pigment, the preparation method comprising the following steps:

[0027] Weigh 5 parts of black carrot dry powder, 10g each, add 0.8% cellulase solution at the ratio of 1:5, 1:10, 1:15, 1:20, 1:25 (g / mL) respectively, and adjust the pH of the solution value to 3.5, the obtained 5 parts of the solution were leached in a constant temperature water bath at 75°C for 1 hour, and then centrifuged at a speed of 4000r / min for 40 minutes, filtered, and the filter residue was leached once, then the filtrate was combined and the volume was adjusted to 200mL; Pipette 5mL of the extract into a 10mL volumetric flask, set the volume to the mark, shake well, adjust the pH value to 6.5, let it stand for 60min, and measure the absorbance value at the maximum absorption wavelength of 533nm; absorb with X-5 resin, and then Elute with ethanol solution to obtain black carotene extract.

Embodiment 2

[0029] A method for optimizing the extraction process of black carotene pigment, the preparation method comprising the following steps:

[0030] Weigh 5 parts of black carrot dry powder, 10g each, add 0.8% cellulase solution at a ratio of 1:15 (g / mL), and adjust the pH value of the solution to 3.5, and divide the obtained 5 parts of the solution at 45, 55, 65 , 75, and 85°C constant temperature water bath for 1 hour, then centrifuged at 4000r / min for 40 minutes, filtered, leached the filter residue once, combined the filtrate and made it to 200mL; precisely pipette 5mL of the extract into In a 10mL volumetric flask, set the volume to the mark, shake well, adjust the pH value to 6.5, let it stand for 60min, and measure the absorbance value at the maximum absorption wavelength of 533nm; adsorb with X-5 resin, and then elute with ethanol solution to obtain Black Carrot Pigment Extract.

Embodiment 3

[0032] A method for optimizing the extraction process of black carotene pigment, the preparation method comprising the following steps:

[0033] Weigh 5 parts of black carrot dry powder, 10g each, add 0.2%, 0.4%, 0.6%, 0.8%, 1.0% cellulase solution at a ratio of 1:15 (g / mL), and adjust the pH value of the solution to 3.5, Extract the obtained 5 solutions in a constant temperature water bath at 75°C for 1 hour, then centrifuge for 40 minutes at a speed of 4000r / min, filter, extract the filter residue once, combine the filtrate and dilute to 200mL; Put 5mL of liquid solution in a 10mL volumetric flask, set the volume to the mark, shake well, adjust the pH value to 6.5, let it stand for 60min, measure the absorbance value at the maximum absorption wavelength of 533nm; absorb with X-5 resin, and then wash with ethanol solution Take off to obtain black carotene extract.

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Abstract

The invention provides a black carrot pigment extraction process optimization method, and the method comprises: weighing black carrot dry powder, adding a cellulase solution according to a ratio of 1:15 (g / mL), adjusting the pH value of the solution to 3.5, leaching in a 75 DEG C constant temperature water bath for 1 h, performing centrifuging for 40 min at a rotation speed of 4,000 r / min, performing filtering, leaching the filter residue once, combining the filtrates, fixing the volume to 200 mL, measuring the light absorption value, and employing X-5 resin for absorption; and performing eluting with an ethanol solution to obtain a black carrot pigment extract. According to the invention, the cellulase is used as an auxiliary extraction solution, the reaction conditions are mild, the quality of the black radish pigment is guaranteed to the maximum extent, the consumption of an organic solvent is low, the extraction efficiency is high, environmental protection and energy conservationare achieved, and large-scale industrial production can be carried out.

Description

technical field [0001] The invention relates to the technical field of plant deep processing, in particular to an optimization method of black carrot pigment extraction process. Background technique [0002] The characteristics of black carrots and carrots are basically the same. The outer skin of the fruit is purple-black, and the center column is purple and occasionally orange-yellow. Black carrot pigments extracted from black carrots belong to anthocyanins, and black carrots have attracted widespread attention due to their rich anthocyanins. Black carrot pigment has a strong antioxidant activity, has anti-aging, anti-cardiovascular disease, anti-cancer, immunity-enhancing and other functions, and also has certain pharmacological and nutritional effects, and is widely used in cosmetics, food, and medicine. It has great development potential and can be used as an ideal substitute material for synthetic pigments. [0003] The extraction of black carrot pigment mainly adopt...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 苏刘花
Owner 南京泽朗医药技术有限公司
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