Probiotic agent PAPCH for preventing and treating helicobacter pylori infection and preparation method of probiotic agent PAPCH

A technology of Helicobacter pylori and probiotics, applied in biochemical equipment and methods, methods based on microorganisms, medical preparations with non-active ingredients, etc., can solve the problem of fewer probiotics and their bacterial agents, and improve human immunity High strength, high strain survival rate, and the effect of improving the micro-ecological environment

Active Publication Date: 2021-03-05
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some probiotics have been found to have an inhibitory effect on Helicobacter pylori, and can reduce the eradication rate of antibiotics and reduce side effects, but there are still few probiotics and their agents that are effective in preventing and treating Helicobacter pylori infection

Method used

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  • Probiotic agent PAPCH for preventing and treating helicobacter pylori infection and preparation method of probiotic agent PAPCH
  • Probiotic agent PAPCH for preventing and treating helicobacter pylori infection and preparation method of probiotic agent PAPCH
  • Probiotic agent PAPCH for preventing and treating helicobacter pylori infection and preparation method of probiotic agent PAPCH

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 PAPCH performance detection

[0050] 1. PAPCH acid resistance test

[0051] PAPCH acid resistant such as figure 1 as shown, figure 1 The histograms in each set of data represent the detection results of pH6.5, pH3.5, pH3, pH2.5, and pH2 from left to right; The number of PAPCH viable bacteria at 3.5 and 6.5. When PAPCH was at pH 3.5 and 3.0, the survival amount of PAPCH was almost the same as that of the blank group (pH 6.5). After 2 hours under the condition of pH 2.5, the number of viable bacteria in PAPCH was significantly reduced, and then the number of viable bacteria was stable at 10 5 On the order of magnitude; after 1 hour under the condition of pH2.0, the number of viable bacteria in PAPCH has been significantly reduced, and the number of viable bacteria in PAPCH has stabilized at 10 after 2 hours. 4 order of magnitude.

[0052] Probiotics are defined as live microorganisms that, when ingested in appropriate amounts, confer a benefit on the host. ...

Embodiment 2

[0061] Example 2 Establishment of PAPCH Prevention Model of Hp Infection

[0062] 1. Preparation of bacterial suspension

[0063] (1) Preparation of PAPCH bacterial suspension

[0064] Take the subcultured PAPCH, centrifuge at 6000rpm for 10min in a centrifuge, take it out, discard the supernatant, wash with normal saline, and adjust the concentration of the bacterial suspension to 2×10 9 CFU / mL, prepared before each gavage to ensure the viability of bacteria.

[0065] (2) Preparation of Helicobacter pylori suspension

[0066] Take the subcultured Helicobacter pylori blood plate, add 1mL / dish of normal saline, scrape off the colony with a coating stick, suck it into a centrifuge tube with a pipette gun, and adjust the concentration of the bacterial suspension to 2×10 9 CFU / mL, prepared before each gavage to ensure the viability of bacteria.

[0067] 2. Mouse experiment

[0068] (1) The first period of gavage: establish a prevention model

[0069] After 7 days of adaptive...

Embodiment 3

[0085] Embodiment 3 fast urease reaction

[0086] Put the gastric tissue in the quick urease test paper, and observe the result within 5 minutes. The result is as Figure 5 as shown, Figure 5 NC is the blank group; PAPCH is the PAPCH treatment group; HP is the H.pylori treatment group; pre-P / post-H is the pre-PAPCH / post-H.pylori treatment group.

[0087] Put the gastric tissue of the mice in the blank group and the PAPCH treatment group on the test paper, and the color of the test paper did not change significantly. Put the Helicobacter pylori treatment group and the pre-P / post-H group on the test paper, and the test paper quickly turned red. And the color of the Helicobacter pylori treatment group was more purple than that of the pre-P / post-H group.

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Abstract

The invention discloses a probiotic agent PAPCH for preventing and treating helicobacter pylori infection and a preparation method of the probiotic agent PAPCH. The probiotic agent PAPCH comprises lactobacillus casei lyT-7 and a protective agent, The preservation number of the lactobacillus casei lyT-7 is CCTCC NO: M 2010197, the preservation time is August 9th, 2010, and the preservation addressis China Center for Type Culture Collection (CCTCC). The addition quantity of the protective agent is 50-150wt% of the use quantity of the lactobacillus casei lyT-7. The prepared probiotic agent can effectively inhibit adhesion of the helicobacter pylori on gastric epithelial cells, inflammatory reaction and immune response caused by the helicobacter pylori, and oxidation reaction induced by helicobacter pylori infection, can directly inhibit and even kill the helicobacter pylori, besides, can improve the micro-ecological environment of gastrointestinal tracts, and can reduce adverse effects of antibiotics.

Description

technical field [0001] The invention belongs to the technical field of preparation of probiotics PAPCH (Probiotics Aimed at Prevention and Control of H. pylori, PAPCH), in particular to a probiotics PAPCH for preventing and treating Helicobacter pylori infection and a preparation method thereof. Background technique [0002] Helicobacter pylori (H. pylori, HP) is a curved spiral Gram-negative bacterium, more than half of the world's population is infected with Helicobacter pylori, and 10%-15% of them develop peptic ulcers, about 1-2% develop gastric cancer. Currently, effective methods for treating Helicobacter pylori infection are triple therapy and quadruple therapy, which eliminate Helicobacter pylori through a combination of antibiotics. However, with the abuse of antibiotics and the increase in the resistance of Helicobacter pylori, the eradication rate of antibiotics to treat Helicobacter pylori infection has been declining year by year, and the combination of antibio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61K35/748A61K47/26A61K47/10A61P31/04A61P1/00C12N1/20C12N1/04C12R1/245
CPCA61K35/747A61K35/748A61K47/26A61K47/10A61P31/04A61P1/00C12N1/20C12N1/04A61K2300/00Y02A50/30
Inventor 赵建彭静珊吴道艳于智豪李姝严馨
Owner SICHUAN UNIV
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