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Composition for improving thin endometrium and preparation method and application thereof

An endometrial and composition technology, applied in the field of medicine, can solve the problems of difficult to supervise the differentiation of stem cells, the treatment effect of thin endometrium is not obvious, and the probability of infection in the uterine cavity is increased, so as to improve the receptivity and blood supply. , Helps endometrial growth, less pain for patients

Pending Publication Date: 2021-03-09
陕西中鸿科瑞再生医学研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Drug treatments such as oral estrogen and aspirin have been taken for a long time, and the side effects are large, and the therapeutic effect is not obvious; surgical treatment such as endometrial scraping and electroacupuncture is invasive, which increases the chance of intrauterine infection and is effective for intractable The treatment effect of the thin endometrium is not obvious, and the patient needs to go to the hospital regularly for operation, and the pain is strong
[0004] Studies have confirmed that mesenchymal stem cells can well promote the repair of thin endometrium in vivo, and this effect is achieved through the paracrine effect, that is, cytokines secreted by cells participate in endometrial repair, but this treatment requires stem cell transplantation , has high technical requirements, and after stem cell transplantation, it is difficult to monitor the differentiation of stem cells in vivo, and the safety needs to be studied

Method used

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  • Composition for improving thin endometrium and preparation method and application thereof
  • Composition for improving thin endometrium and preparation method and application thereof
  • Composition for improving thin endometrium and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A composition for improving thin endometrium, which is composed of endometrial mesenchymal stem cell supernatant and vitamin E, and the amount of vitamin E added to the endometrial mesenchymal stem cell culture supernatant is 5 mg / mL ;

[0028] Among them, endometrial mesenchymal stem cell supernatant is low-generation cells, and derived from menstrual blood, using menstrual blood as a raw material can also play a role in effective waste utilization; more preferably, endometrial mesenchymal stem cell supernatant The menstrual blood from patients with thin uterus and the supernatant of endometrial mesenchymal stem cells from the patients themselves have better compatibility and are safer and more effective.

[0029] The primary isolation and culture methods of the above-mentioned endometrial mesenchymal stem cells are as follows:

[0030] (1) Collect women's menstrual period (2-3d) menstrual blood, add 2 times the volume of PBS buffer and mix well, centrifuge at 1000rpm...

Embodiment 2

[0039] A composition for improving thin endometrium, the difference from Example 1 is that the amount of vitamin E added to the culture supernatant of endometrial mesenchymal stem cells is 20 mg / mL.

[0040] The preparation method of the above-mentioned composition for improving thin endometrium comprises the following steps:

[0041] Dry cells at 37°C, 5% CO 2 When the incubator is cultivated to a density of 75%, discard the original culture medium, wash it twice with phosphate buffer, then add serum-free medium to continue culturing overnight, collect the cell culture supernatant, centrifuge at 1500rpm, and collect the serum-free culture supernatant. Then, the serum-free culture supernatant was concentrated through a 50KD ultrafiltration concentrator tube for protein content detection. After passing the test, the stem cell culture supernatant was obtained, then subpackaged and frozen for future use, and vitamin E was added at 20 mg / mL before use.

[0042]The above-mentioned...

Embodiment 3

[0044] A composition for improving thin endometrium, the difference from the above-mentioned embodiments is that the mesenchymal stem cells used are adipose-derived mesenchymal stem cells, and the amount of vitamin E added to the culture supernatant of adipose-derived mesenchymal stem cells is 30mg / mL.

[0045] The preparation method of the above-mentioned composition for improving thin endometrium comprises the following steps:

[0046] Dry cells at 37°C, 5% CO 2 When the incubator is cultivated to a density of 70%-80%, discard the original culture medium, wash it twice with phosphate buffer, then add serum-free medium to continue the culture overnight, collect the cell culture supernatant, centrifuge at 4000rpm, and collect the Serum culture supernatant, and then concentrate the serum-free culture supernatant through a 50KD ultrafiltration concentrator tube for protein content detection, obtain stem cell culture supernatant after passing the test, then subpackage, freeze an...

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Abstract

The invention belongs to the technical field of medicine, and particularly discloses a composition for improving thin endometrium and a preparation method and application thereof. The composition is prepared from stem cell culture supernatant and vitamin E. After the composition for improving thin endometrium is used, expression of VEGF and integrin beta 3 in endometrium can be enhanced, the thickness of endometrium is increased, and uterus receptivity and blood supply are improved.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically discloses a composition for improving thin endometrium, a preparation method and application thereof. Background technique [0002] Thin endometrium refers to the endometrial thickness in the mid-luteal phase of ≤7mm, and the pathological features show slow growth of glandular epithelium, high blood flow resistance of uterine arteries, poor angiogenesis and low expression of vascular endothelial growth factor. Studies have shown that the receptivity of the thin uterus decreases during the window implantation period (20-24 days of menstruation), and the expression of vascular endothelial cell growth factor VEGF and integrin β3 is severely lacking, which affects embryo implantation and implantation, and is the leading cause of clinical infertility. high incidence factors. [0003] At present, the treatment of thin endometrium mainly includes drugs to stimulate endometrial hyperpl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K31/355A61P15/00
CPCA61K35/28A61K31/355A61K9/0034A61P15/00A61K2300/00
Inventor 董玲娟明磊国王清霞
Owner 陕西中鸿科瑞再生医学研究院有限公司
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