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Application of mitochondrial function intervention material in preparation of health care products or drugs for improving mitochondrial function and screening method thereof

A screening method, mitochondrial technology, applied in the field of health care products or pharmaceuticals, can solve the problems of mixed products, single detection means, incomplete detection, etc., to improve mitochondrial function, high construction success rate, and obvious effect Effect

Pending Publication Date: 2021-03-09
BY HEALTH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are problems such as inconsistent detection indicators, backward detection technology, single detection means and incomplete detection in the functional determination of mitochondria-related nutritional supplements.
These problems have caused the quality of related products on the market to be uneven, and the phenomenon of mixed genuine and fake products

Method used

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  • Application of mitochondrial function intervention material in preparation of health care products or drugs for improving mitochondrial function and screening method thereof
  • Application of mitochondrial function intervention material in preparation of health care products or drugs for improving mitochondrial function and screening method thereof
  • Application of mitochondrial function intervention material in preparation of health care products or drugs for improving mitochondrial function and screening method thereof

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Embodiment Construction

[0054] The present invention will be further described below through specific embodiments. The following examples are specific embodiments of the present invention, but the embodiments of the present invention are not limited by the following examples.

[0055] 1. Experimental method

[0056] 1. Cell culture

[0057] The cell line is C2C12 cells (mouse myoblasts);

[0058] Cell growth medium: DMEM complete medium containing 10% FBS (fetal bovine serum), 1% P / S (double antibody, 100U / mL penicillin and 100 μg / mL streptomycin);

[0059] Cell differentiation medium: DMEM complete medium containing 2% HS (horse serum), 1% P / S;

[0060] Growth conditions: 37°C, 5% CO 2 .

[0061] 2. Cell passage

[0062] Subculture when the cell fusion rate reaches 80-85%:

[0063] (1) Take out the cells in the incubator, observe under the microscope whether they need to be passaged, put them into the ultra-clean bench, then discard the old culture medium in the culture dish, and add 1-2 mL PB...

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Abstract

The invention discloses application of a mitochondrial function intervention material in preparation of health care products or drugs for improving mitochondrial function and a screening method thereof. The method includes: constructing a mitochondrial damage model by palmitic acid; determining acyl carnitine by LC-MS / MS method; detecting respiratory function by a Seahorse extracellular flux analyzer; conducting quantitative PCR detection on mitochondrial DNA content; conducting real-time quantitative PCR detection on mRNA level expression of genes mainly related to mitochondria; detecting mitochondrial related genes and oxidized phosphorylated complex protein in skeletal muscle by Western blot method; detecting MDA content and NAD+ / NADH content by a kit; and observing the ultrastructure of cell mitochondria by a transmission electron microscope and conducting morphological analysis. Compared with the existing evaluation screening system, the evaluation screening system provided by theinvention is more scientific, accurate and efficient.

Description

technical field [0001] The invention belongs to the field of health care products or medicines, and in particular relates to the application and screening method of mitochondrial function intervention materials in the preparation of health care products or medicines for improving mitochondrial function. Background technique [0002] Mitochondria, an essential building block of eukaryotic cells, have long been considered the energy factories within the cell, responsible for providing the cell with the energy it needs to perform its daily functions. The energy required by human cells is mainly provided by mitochondria. It is worth noting that as we age, the function of mitochondria deteriorates. However, more and more studies have found that mitochondria not only function as "cell energy stations", they are involved in the regulation of various cellular functions, and are closely related to many human diseases, including cell signal transduction, metabolism, autophagy, aging a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K38/01A61K31/706A61P3/00C12Q1/02A23L33/13A23L33/18A23L33/105
CPCA61K45/00A61K38/012A61K31/706A61P3/00G01N33/5079G01N33/5038G01N33/5041A23L33/13A23L33/18A23L33/105G01N2500/10A23V2002/00A23V2200/30A23V2250/21A23V2250/21168A23V2250/21164A23V2250/55
Inventor 李言王立聂陈志鹏贺瑞坤张旭光
Owner BY HEALTH CO LTD
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