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A kind of extraction and purification method of monascus orange pigment

An orange pigment and extraction method technology, applied in the field of biochemistry, can solve the problems of large amount of solvent, high pressure on safety and environmental protection, and low extraction rate, and achieve the effects of less solvent amount, easy separation, and improved extraction rate

Active Publication Date: 2022-05-03
STAR LAKE BIOSCI CO INC ZHAOQING GUANGDONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using column chromatography to separate and extract monascus orange pigment, the amount of solvent used is large, the pressure on safety and environmental protection is high, and the extraction rate is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Monascus fermented liquid is centrifuged to obtain wet mycelium of red yeast rice. The wet mycelium of red yeast rice is directly broken through a ball mill, and the degree of wall breaking of mycelium reaches 70%. According to the amount of wet mycelium, the mass ratio is 1:1 Add micropowdered silica gel, then put it into the extraction kettle of supercritical carbon dioxide equipment, heat the temperature of the extraction kettle to 55°C and the temperature of the separation kettle to 45°C, pump carbon dioxide into the extraction kettle and the separation kettle with a high-pressure pump, and adjust the valve to make the extraction kettle , Separating kettle reach 20MPa, 10MPa respectively, start circulation extraction, after the setting parameter is stable, pump into the ethanol 1.5ml that volume fraction is 95% and start timing, the consumption of entrainer in every gram of red yeast rice mycelia is 1.5mL, The extraction time is 120min. After the extraction is comple...

Embodiment 2

[0011] Monascus fermented liquid is centrifuged to obtain wet mycelium of red yeast rice. The wet mycelium of red yeast rice is directly broken through a ball mill, and the degree of wall breaking of mycelium reaches 70%. According to the amount of wet mycelium, the mass ratio is 1:1 Add micropowdered silica gel, then put it into the extraction kettle of supercritical carbon dioxide equipment, heat the temperature of the extraction kettle to 45°C and the temperature of the separation kettle to 40°C, pump carbon dioxide into the extraction kettle and the separation kettle with a high-pressure pump, and adjust the valve to make the extraction kettle , separation kettle reach 22MPa, 10MPa respectively, start circulation extraction, after the setting parameters are stable, pump into 2.0ml of ethanol with a volume fraction of 70% and start timing, the consumption of entrainer in every gram of Monascus mycelium is 2.0mL, The extraction time was 120min. After the extraction was comple...

Embodiment 3

[0013] Monascus fermented liquid is centrifuged to obtain wet mycelium of red yeast rice. The wet mycelium of red yeast rice is directly broken through a ball mill, and the degree of wall breaking of mycelium reaches 70%. According to the amount of wet mycelium, the mass ratio is 1:1 Add micropowdered silica gel, then put it into the extraction kettle of supercritical carbon dioxide equipment, heat the temperature of the extraction kettle to 60°C and the temperature of the separation kettle to 45°C, pump carbon dioxide into the extraction kettle and the separation kettle with a high-pressure pump, and adjust the valve to make the extraction kettle , separation kettle reach 35MPa, 10MPa respectively, start circulation extraction, after the setting parameter is stable, pump into the ethanol 2.0ml that volume fraction is 95% and start timing, the consumption of entrainer in every gram of red yeast rice mycelia is 2.0mL, The extraction time was 120min. After the extraction was comp...

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Abstract

The invention belongs to the technical field of biochemistry, and specifically relates to a method for extracting and purifying monascus orange pigment. The steps are: A. The red yeast rice fermentation liquid is separated by tubular centrifugation to obtain the wet mycelium of the red yeast rice. The centrifugal speed is 10000-14000r / min, and the centrifugation time is 5-15mm; B. The wet mycelium of the red yeast rice obtained in step A is The body is directly broken through the ball mill. When the broken degree of the wet mycelium of the red yeast rice reaches 50-70%, according to the amount of the wet mycelium, add micropowdered silica gel at a mass ratio of 1:1, and then put it into a supercritical carbon dioxide extraction device for extraction. Monascus orange pigment; C, discharge from the separation tank after extraction and collect the monascus orange pigment extract. The invention adopts the supercritical carbon dioxide extraction technology, improves the extraction rate, shortens the extraction time, and has high purity and high extraction rate of the monascus orange pigment.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and specifically relates to a method for extracting and purifying monascus orange pigment. Background technique [0002] Monascus pigment is a metabolite of Monascus, mainly composed of yellow pigment, red pigment and orange pigment. Monascus pigment has three shades: orange, yellow, and red, and monascus pigment with an orange hue is called monascus orange pigment. Monascus orange pigment is bright in color and has good safety. It is a kind of natural coloring agent with development prospect, which can be used in industries such as food, medicine and fine chemical industry. Monascus orange pigment is also a major antibacterial substance produced by Monascus, which can inhibit Gram-positive bacteria and Escherichia coli. The currently reported methods for the separation and purification of monascus orange pigment in the laboratory mainly include thin-layer chromatography, column chromatogr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00C09B67/54C12N1/06C12R1/645
CPCC09B61/00C09B67/0096C12N1/066Y02P20/54
Inventor 李嫦王海波谢畅丰严盟孙艳萍
Owner STAR LAKE BIOSCI CO INC ZHAOQING GUANGDONG
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