Fluorescence Spectrum Detection Method Based on Smartphone

Abstract

A smart phone-based fluorescence spectrum detection method involves spectrum detection and image processing. 1) Fluorescent signal detection: use the camera of the smart phone as a detector to detect the fluorescent signal; 2) Image data processing: process the video data captured by the smart phone; The color channel information of the effective light-emitting area of ​​the sample fluorescence image, select the appropriate color value as the fluorescence intensity parameter of the sample, take the wavelength as the abscissa, and the fluorescence intensity as the ordinate, draw the characteristic fluorescence spectrum curve of the sample; the characteristic fluorescence spectrum curve of the sample After generation, save it in the specified folder with a custom name, and generate a fluorescent substance spectral database. Realize the rapid and accurate scanning of the fluorescence spectrum of the substance in the form of video through the image sensor of the smartphone camera; the operation is simple, the analysis time is short, and the detection sensitivity can be improved by reducing the interference of environmental noise.

Description

technical field [0001] The invention relates to the technical field of spectrum detection and image processing, in particular to a smart phone-based fluorescence spectrum detection method. Background technique [0002] Fluorescence analysis has the advantages of high sensitivity, strong selectivity and various methods, and has been widely used in the fields of chemistry, biology, medicine, health, agriculture and environmental protection. As a characterization technique, fluorescence spectroscopy is used to study the physical and chemical properties and changes of the system, and can be used as a means of qualitative detection and quantitative analysis. The operation of the traditional fluorescence analysis method is relatively complicated, which is not conducive to popularization, and the instrument is large in size and high in manufacturing cost, making it difficult to apply to on-site detection. Therefore, the research on new rapid fluorescence analysis technology and po...

AI Technical Summary

Problems solved by technology

[0004] The photodetectors used in traditional fluorescence analysis instruments are mainly PMTs (photomultiplier tubes), which need to provide a stable negative high voltage device, making it difficult to miniaturize the instrument

At present, the devices used in portable spectral detection methods based on smartphones often require additional optical components, the optical path design is relatively complicated, and the cost is high

At the same time, the existing spectral detection method based on smartphones acquires the spectrum by taking a single sample picture, which has shortcomings such as small detection range, single detection object, complex image processing, and long analysis time.

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