Florfenicol rapid detection method
A technology of florfenicol and a detection method, which is applied in the field of rapid detection of florfenicol, can solve the problems of cumbersome processing and measurement operations, long time required for detection, restrictions on popularization and use, etc., and achieves reliable quantitative analysis results and improved performance. Anti-interference ability, the effect of reducing detection cost
Inactive Publication Date: 2021-03-09
苏州慧源安食检测科技有限公司
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Problems solved by technology
Detect whether there are antiviral drug residues in pig hair, so as to achieve the purpose of controlling the use of banned drugs from the source of breeding. Currently, florfenicol generally uses HPLC or LC / MS, but the sample pretreatment and measurement operations of this method are cumbersome and difficult to detect. It takes a long time, the detection cost is high, and the popularization and use are limited. Therefore, research and establishment of a detection method for florfenicol drug residues in pig hair is of great importance for effectively monitoring the use of this type of drug and ensuring the safety of animal food in our country. significance
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[0030] Example: such as Figure 1-4 Shown, the rapid detection method of a kind of Florfenicol of the present invention comprises the following steps:
[0031] Step 1: pretreatment, cleaning the sample and extracting the organic solvent;
[0032] Step 2: Add 5 ml of acetonitrile ethyl acetate to the organic solvent extract, shake fully for about 30 seconds, oscillate through a vortex shaker, then add 10 ml of ethyl acetate, vortex for 2 minutes, and centrifuge at 5000r / min 5 minutes;
[0033] Step 3: Dry the clear liquid with nitrogen at a temperature of 60°C, add 1 ml of water to dissolve the residue, vortex for 30 seconds, and filter through a filter membrane to obtain the test liquid;
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The invention discloses a florfenicol rapid detection method. The method comprises the following steps: 1, carrying out pretreatment, cleaning a sample, and extracting an organic solvent; and 2, adding 5ml of acetonitrile ethyl acetate into the extracting solution of the organic solvent, fully oscillating for about 30 seconds, oscillating by using a vortex oscillator, adding 10ml of ethyl acetate,uniformly mixing for 2 minutes in a vortex manner, and centrifuging for 5 minutes at the speed of 5000r / min. According to the florfenicol rapid detection method, a large amount of fat and other endogenous interferents are removed by washing hydrolysate with ethyl acetate in s sample pretreatment process; and the method has the advantages of substantial improvement of an anti-interference abilityof the detection method on other substances in a pig edible tissue, reduction of the detection cost, reduction of the use amount of an organic solvent, a reliable quantitative analysis result, and suitableness for daily monitoring of florfenicol total residues in the pig edible tissue by basic detection units, and quantification by an internal standard method.
Description
technical field [0001] The invention relates to the technical field of florfenicol detection, in particular to a rapid detection method of florfenicol. Background technique [0002] Florfenicol, also known as florfenicol, is a fluorinated derivative of chloramphenicol. Its molecular structure has no potential aplastic anemia-causing nitro group, and it has a broad antibacterial spectrum and is easy to absorb. Therefore, florfenicol has become the main substitute of chloramphenicol and is widely used in the treatment of gram-positive bacteria, gram-negative bacteria and chloramphenicol-resistant bacteria in animals. However, the reproductive toxicity test results show that florfenicol has hemotoxicity, embryotoxicity and immunosuppressive effects, and adverse reactions such as anorexia and diarrhea will also occur after medication. Eating a large amount of pork with excessive florfenicol will bring harm to human health. Great harm. At present, due to the improper clinical u...
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IPC IPC(8): G01N21/78G01N1/34G01N1/38
CPCG01N21/78G01N1/34G01N1/38G01N2021/7759G01N2001/386
Inventor 卞禹卜
Owner 苏州慧源安食检测科技有限公司