An electrochemiluminescent method for detecting nitrogen-doped carbon quantum dots

A carbon quantum dot, electrochemical technology, applied in the field of electrochemical detection, can solve the problem of no relevant reports, and achieve the effects of high sensitivity, low cost, and short detection time

Active Publication Date: 2022-06-21
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There are currently no reports on this method

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  • An electrochemiluminescent method for detecting nitrogen-doped carbon quantum dots
  • An electrochemiluminescent method for detecting nitrogen-doped carbon quantum dots
  • An electrochemiluminescent method for detecting nitrogen-doped carbon quantum dots

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Embodiment 1

[0026] In an electrochemiluminescence cell containing 1 mL of 0.2 M PBS (pH 7.5) solution, 32 μL of 10 mM solution of carboxylated ruthenium terpyridine was added, and 968 μL of deionized water was added, wherein the concentration of carboxylated ruthenium terpyridine was 0.16 mM. The mixed solution was scanned by the electrochemiluminescence signal through the three-electrode system under the parameters of the voltage of 0.2V-1.25V, the scan rate of 100mV / s, and the high voltage of the photomultiplier tube of 700V, to obtain the carboxylated terpyridine ruthenium solution B the luminous signal;

[0027] Different amounts of nitrogen-doped carbon quantum dots were sequentially added to the above mixed solution, fully dissolved, and mixed to obtain mixed solutions with different NCQDs concentrations; wherein, the NCQDs concentrations were 0.01 mg / mL, 0.05 mg / mL and 0.1 mg / mL. mL, and the mixed solution of different concentrations of NCQDs was scanned by the three-electrode syst...

Embodiment 2

[0030] In an electrochemiluminescence cell containing 1 mL of 0.2M PBS (pH 7.5) solution, add 32 μL of 10 mM carboxylated ruthenium terpyridine solution and 200 μL of 100 μM tripropylamine (TPA) solution, add 768 μL of deionized water, and mix to obtain Mixed solution A, wherein the concentration of carboxylated ruthenium terpyridine in mixed solution A is 0.16 mM, and the concentration of tripropylamine is 10 μM. The mixed solution A was scanned for electrochemiluminescence signals through a three-electrode system under the parameters of a voltage of 0.2V to 1.25V, a scan rate of 100mV / s, and a photomultiplier tube high voltage of 700V, to obtain information about carboxylated ruthenium terpyridine and The luminescence signal of tripropylamine mixed solution D;

[0031] Different amounts of nitrogen-doped carbon quantum dots were sequentially added to mixed solution A, fully dissolved and mixed to obtain mixed solution B with different NCQDs concentrations; in mixed solution ...

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Abstract

The invention belongs to the field of electrochemical detection and relates to an electrochemical luminescence method for detecting nitrogen-doped carbon quantum dots. In the present invention, based on tripropylamine (TPA) as carboxylated terpyridine ruthenium (Ru(dcbpy) 3 2+ ), using nitrogen-doped carbon quantum dots (NCQDs) for Ru(dcbpy) 3 2+ The quenching effect of the / TPA system realizes the detection of NCQDs. NCQDs vs. Ru(dcbpy) 3 2+ The quenching behavior of the ECL signal of / TPA is mainly attributed to Ru(dcbpy) 3 2+ / NCQDs interactions, but not TPA / NCQDs. Specifically, when Ru(dcbpy) 3 2+ When coexisting with NCQDs, Ru(dcbpy) 3 2+ The carboxyl groups in the NCQDs fully contact with the oxygen-containing and nitrogen-containing groups on the surface of NCQDs, forming intermolecular hydrogen bonds, absorbing the energy of the system, and weakening the Ru(dcbpy) 3 2+ the ECL signal. On this basis, a quenched ECL sensor for the detection of NCQDs was developed with a linear range of 0.002-0.2 mg / mL ‑1 , the detection limit is 0.001mg / mL, and the high-performance analysis of NCQDs has been successfully realized. The method is fast, simple and sensitive.

Description

technical field [0001] The invention relates to an electrochemiluminescence method for detecting nitrogen-doped carbon quantum dots. The method has the characteristics of rapidity, simplicity and sensitivity, and belongs to the field of electrochemical detection. Background technique [0002] Carbon quantum dots (CQDs) are a class of nanoparticles with the advantages of nano-size effect, high water dispersibility, and high fluorescence stability, which have been widely used in biosensing, bioimaging, drug carriers, and photocatalysis. However, CQDs will inevitably enter the natural environment during the production, use, disposal and recycling process, and the potential ecological impact of their high water dispersibility on the natural environment, especially the water environment, needs to be paid attention to. According to literature reports, high concentrations of CQDs can cause physical damage and oxidative stress to cells and bacteria, cause growth inhibition to algae,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N21/76
CPCG01N27/30G01N21/76
Inventor 由天艳刘晓红李丽波罗莉君毕晓雅
Owner JIANGSU UNIV
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