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A kind of masson pine bark extract and its preparation method and application
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A kind of pine bark extract, extract technology, applied in the field of extraction of plant active ingredients
Active Publication Date: 2022-07-19
国珍健康科技(北京)有限公司
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At present, most of the research is on the anti-oxidation, anti-tumor, and blood-lipid-lowering effects of the bark extract of Pinus massoniana, and there is no report that its extract has anti-photooxidation effect
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Embodiment 1
[0077] The present embodiment relates to a preparation method of masson pine bark extract, comprising the following steps:
[0078] (1) Selected masson pine bark, washed, dried at 50°C, and smashed through an 80-mesh sieve; 3% cellulase (CAS: 9012-54-8; gray-white amorphous powder) was added to the above-mentioned masson pine bark powder. , add distilled water according to the ratio of material to liquid 1:7, adjust the pH to 5.0 with dilute hydrochloric acid, and incubate at 50°C for 100min;
[0079] (2) adding dehydrated alcohol and distilled water to above-mentioned system, making ethanol concentration be 60%, ultrasonic treatment 50min, ultrasonic power is 300W, carry out suction filtration to mixed material, collect extract;
[0080] (3) Repeat the operation of the residue according to step 2 once; and combine the two extracts; concentrate under reduced pressure at 45°C to 1 / 10 of the original volume to obtain a concentrated solution;
[0081] (4) the concentrated soluti...
Embodiment 2
[0085] The difference between this example and Example 1 is that an extract with stronger anti-photoaging ability is obtained by separating and purifying the component Fr.2.
[0086] Take step component Fr.2, add a small amount of absolute ethanol to dissolve, and add distilled water to make a suspension; use polyamide resin for purification, and the polyamide particle size is 200 mesh; the diameter-height ratio of the polyamidechromatography column is 1 : 6; the elution volume is 2.5BV;
[0087] The ethanol:water solventsystem was eluted according to the volume ratio of 20:80, 60:40, and 100:0. The cell results showed that the eluted fraction of 60:40 had better anti-photoaging effect. Decompose, remove moisture and dry to obtain powder.
Embodiment 3
[0089] The difference between this example and Example 2 is that an extract with stronger anti-photoaging ability is obtained by purifying the components described in Example 2 (the extraction flow chart is as follows: figure 1 ).
[0090] The components obtained in Example 2 were dissolved in a small amount of methanol, prepared into a solution with a concentration of 10 mg / mL, passed through a 0.45 μm filter membrane, and separated and purified by a medium-pressure preparative chromatography device. The sample volume is 2% of the column volume, and methanol solutions of different concentrations are used for elution, wherein the eluent gradient is: 2CV, 20-40%; 3CV, 40-80%; 1CV, 80%; 1CV, 80-100 %; 2CV, 100-100%, the instrument automatically collects the peak components. HPLC detected and combined similar components to obtain a total of 4 components Fr.2.1~Fr.2.4, which were concentrated under reduced pressure at 45°C and lyophilized to obtain solid powder; after verificatio...
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Abstract
The invention belongs to the field of extraction of plant active ingredients, and in particular relates to a method for preparing a masson pinebark extract, comprising the following steps: 1) crushing the masson pinebark and adding cellulase for enzymatic hydrolysis to obtain pretreated materials; 2) Add methanol or ethanol solution to the pretreatment material, and carry out ultrasonic treatment to the mixed system to extract the effective components in the pretreatment material, and collect the extract; 3) sequentially purify the mixture with ethyl acetate and water-saturated n-butanol; The extract is extracted, and the extract of water-saturated n-butanol is collected; 4) the active ingredient in the water-saturated n-butanol extract is separated and purified with a macroporous adsorption resin of weak polarity. The ethanol solution is used as the eluent for elution, and the eluent with a volume fraction of ethanol greater than 30% is collected, concentrated and dried to obtain the masson pinebark extract. The extract obtained by the method of the present invention has the effect of resisting photoaging.
Description
technical field [0001] The invention belongs to the field of extraction of plant effective components, in particular to a masson pine bark extract and its application in anti-photoaging. Background technique [0002] As the body's first line of defense against external aggressions, the skin is susceptible to photoaging caused by ultravioletradiation. Ultraviolet rays will induce skin cells to produce a large number of free radicals. Due to the high activity of free radicals and strong oxidative reaction ability, the expression levels of cytokines, receptors and enzymes will be unregulated, and the NF-kB and MAPK pathways will be excessively activated. DNA It can cause skin wrinkles, pigmentation, relaxation and sunburn and other symptoms, and in more serious cases, it may cause genemutation and induce skin cancer. The severity of photoaging depends primarily on the cumulative dose of UV exposure received and the pigmentation status of the skin. Photoaging of the skin is ...
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